AB133635

Anti-ACY-1 antibody [EPR8445]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal ACY-1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Aminoacylase-1, ACY-1, N-acyl-L-amino-acid amidohydrolase, ACY1

2 Images

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Western blot - Anti-ACY-1 antibody [EPR8445] (AB133635)

All lanes:

Western blot - Anti-ACY-1 antibody [EPR8445] (ab133635) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 46 kDa

Observed band size: 42 kDa

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OI-RD Scanning - Anti-ACY-1 antibody [EPR8445] (AB133635)

OI-RD Scanning

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OI-RD Scanning - Anti-ACY-1 antibody [EPR8445] (AB133635)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-ACY-1 antibody [EPR8445] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8445

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Please note that due to the autogeneration of the immunogen section text, the amino acid number range reads as aa 350-450. This is just to cover the range of the immunogen whilst keeping it proprietary. The uniprot # is correct.

(Q03154 is 408 amino acids long)

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ACY-1 also known as aminoacylase 1 or ACY plays a significant role in the deacetylation process of N-acyl amino acids. ACY-1 is a zinc-binding enzyme and has a molecular weight of approximately 45 kDa. This enzyme is expressed in various tissues including the liver and kidney. ACY-1 is essential for amino acid metabolism by catalyzing the hydrolysis of N-acyl-L-amino acids into L-amino acids and free fatty acids.

Biological function summary

The aminoacylase 1 enzyme functions in detoxification and metabolic regulation. It is not typically part of large protein complexes but acts independently to regulate amino acid and acetyl-CoA pathways. The activity facilitates the recycling of acyl groups and amino acids which is important for maintaining cellular homeostasis.

Pathways

Aminoacylase 1 is involved in amino acid catabolism and lipid metabolism pathways. In these processes ACY-1 interacts with proteins such as acetyl-CoA synthetase in the conversion of excess acyl-CoAs into free fatty acids and CoA preventing the build-up of toxic metabolites. It also has an indirect relationship with the urea cycle by ensuring the availability of free amino acids for further metabolism.

Alterations in ACY-1 function relate to neurological disorders and metabolic syndromes. For instance defects in ACY-1 have been associated with Canavan disease a condition affecting the brain. The protein's activity imbalance might also link to metabolic disorders where its interaction with proteins like aspartoacylase comes into play further affecting the acetylation status and metabolic regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of N-acetylated amino acids to acetate and free amino acids.

See full target information Aminoacylase-1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 13:3397-3406 PubMed39145058

2024

MiR-1271-5p promotes the growth and migration of neuroblastoma cells by regulating ACY-1.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Sun,Xiaowen Zhang,Zimin Chen,Xiaoshuo Ye,Chen Zhang

Scientific reports 14:7244 PubMed38538745

2024

White and gray matter integrity evaluated by MRI-DTI can serve as noninvasive and reliable indicators of structural and functional alterations in chronic neurotrauma.

Applications

Unspecified application

Species

Unspecified reactive species

Lan-Wan Wang,Kuan-Hung Cho,Pi-Yu Chao,Li-Wei Kuo,Chia-Wen Chiang,Chien-Ming Chao,Mao-Tsun Lin,Ching-Ping Chang,Hung-Jung Lin,Chung-Ching Chio

JCI insight 6: PubMed33591955

2021

Proteomic profiling reveals biomarkers and pathways in type 2 diabetes risk.

Applications

Unspecified application

Species

Unspecified reactive species

Debby Ngo,Mark D Benson,Jonathan Z Long,Zsu-Zsu Chen,Ruiqi Wang,Anjali K Nath,Michelle J Keyes,Dongxiao Shen,Sumita Sinha,Eric Kuhn,Jordan E Morningstar,Xu Shi,Bennet D Peterson,Christopher Chan,Daniel H Katz,Usman A Tahir,Laurie A Farrell,Olle Melander,Jonathan D Mosley,Steven A Carr,Ramachandran S Vasan,Martin G Larson,J Gustav Smith,Thomas J Wang,Qiong Yang,Robert E Gerszten

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ACY-1 antibody [EPR8445]

Western blot - Anti-ACY-1 antibody [EPR8445] (AB133635)

OI-RD Scanning - Anti-ACY-1 antibody [EPR8445] (AB133635)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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