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AB254958

Anti-acyl-CoA Thioesterase 2 antibody

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Rabbit Polyclonal acyl-CoA Thioesterase 2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACOT2 aa 1-100.

View Alternative Names

PTE2, PTE2A, ACOT2, Acyl-CoA thioesterase 2, Acyl-coenzyme A thioester hydrolase 2a, CTE-Ia, Long-chain acyl-CoA thioesterase 2, ZAP128

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-acyl-CoA Thioesterase 2 antibody (AB254958)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-acyl-CoA Thioesterase 2 antibody (AB254958)

Paraffin-embedded human kidney tissue stained for acyl-CoA Thioesterase 2 using ab254958 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-acyl-CoA Thioesterase 2 antibody (AB254958)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-acyl-CoA Thioesterase 2 antibody (AB254958)

Paraffin-embedded human skin tissue stained for acyl-CoA Thioesterase 2 using ab254958 at 1/500 dilution in immunohistochemical analysis. Low expression as expected.

Western blot - Anti-acyl-CoA Thioesterase 2 antibody (AB254958)
  • WB

Supplier Data

Western blot - Anti-acyl-CoA Thioesterase 2 antibody (AB254958)

Primary antibody used at 0.4μg/ml.

All lanes:

Western blot - Anti-acyl-CoA Thioesterase 2 antibody (ab254958)

Lane 1:

Human cell line RT-4

Lane 2:

Human cell line U-251 MG

Lane 3:

Human plasma

Lane 4:

Human liver tissue

Lane 5:

Western blot

Lane 5:

Human tonsil tissue

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ACOT2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P49753

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acyl-CoA Thioesterase 2 (ACOT2) also known as long-chain acyl-CoA thioesterase is an enzyme that breaks down fatty acyl-CoA molecules into free fatty acids and CoA. It operates mainly in the mitochondria. ACOT2 has a molecular mass of approximately 48 kDa. Expression of ACOT2 occurs prominently in tissues with high fatty acid oxidation rates like the liver heart and skeletal muscle suggesting a significant role in energy metabolism.
Biological function summary

ACOT2 regulates the intracellular levels of acyl-CoA and free CoA influencing lipid metabolism and energy homeostasis. It is not part of any large protein complex but interacts functionally with other enzymes involved in fatty acid metabolism. ACOT2 prevents the accumulation of toxic acyl-CoA levels that can affect cellular function. Its enzymatic action ensures a balance between lipid synthesis and degradation.

Pathways

ACOT2 plays an integral role in fatty acid β-oxidation and lipid metabolism pathways. These pathways are important for energy production especially during prolonged fasting or increased energy demand. ACOT2 interacts with other enzymes like carnitine palmitoyltransferase 1 (CPT1) and acyl-CoA dehydrogenase facilitating the transport and breakdown of fatty acids. Such interactions highlight its central role in maintaining metabolic homeostasis.

ACOT2 has links to metabolic conditions such as obesity and type 2 diabetes. Altered activity of ACOT2 may contribute to dysfunctional lipid metabolism observed in these disorders. The enzyme relates closely with peroxisome proliferator-activated receptor alpha (PPARα) a protein that regulates genes involved in fatty acid metabolism. Modulation of ACOT2 activity or expression could emerge as therapeutic targets for these metabolic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed : 10944470, PubMed : 16940157). Displays higher activity toward long chain acyl CoAs (C14-C20) (PubMed : 10944470, PubMed : 16940157). The enzyme is involved in enhancing the hepatic fatty acid oxidation in mitochondria (By similarity).
See full target information ACOT2
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