Rabbit Polyclonal Acyl-CoA Thioesterase 9 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACOT9 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ACOT9 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y305
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Target data
Function
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs.
Alternative names
CGI-16, ACOT9, Acyl-CoA thioesterase 9, Acyl-CoA thioester hydrolase 9
Recommended products
Rabbit Polyclonal Acyl-CoA Thioesterase 9 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACOT9 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ACOT9 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y305
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Acyl-CoA Thioesterase 9 (ACOT9) sometimes referred to as anti-CoA functions mechanically by hydrolyzing CoA esters into free fatty acids and CoA-SH playing a role in lipid metabolism. ACOT9 has a molecular mass of approximately 70 kDa. The expression of ACOT9 occurs in various tissues with higher levels observed in the liver and kidney contributing to the regulation of fatty acid and CoA concentrations within the cells.
- Biological function summary
ACOT9 participates in the regulation of lipid metabolism and energy homeostasis. It does not form part of a known complex but performs a stand-alone role in converting activated fatty acids into their free form and CoA. This process contributes to the balance between synthesis and degradation of lipids impacting energy production and storage. ACOT9's action influences cellular metabolic states and can adapt them depending on the energy requirement of the organism.
- Pathways
ACOT9 plays an important role in the peroxisomal and mitochondrial fatty acid oxidation pathways. In these pathways it interacts with enzymes such as Acyl-CoA oxidase and Carnitine palmitoyltransferase I facilitating the breakdown of long-chain fatty acids. ACOT9's hydrolytic activity helps reduce the build-up of acyl-CoA intermediates which is essential for maintaining cellular energy balance and supporting metabolic flexibility during fasting or exercise.
- Associated diseases and disorders
ACOT9 has been linked to metabolic syndromes including obesity and non-alcoholic fatty liver disease (NAFLD). Its modulation can influence the aberrant accumulation of lipids leading to these conditions. ACOT9's activity also connects it with proteins such as AMP-activated protein kinase (AMPK) in the regulation of energy homeostasis which is disrupted in metabolic disorders. Understanding the role of ACOT9 in these diseases may provide insights into therapeutic targets for managing metabolic imbalances.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Acyl-CoA Thioesterase 9 antibody (ab127018)
10% SDS PAGE
All lanes: Western blot - Anti-Acyl-CoA Thioesterase 9 antibody (ab127018) at 1/500 dilution
All lanes: U-87 MG whole cell lysate at 30 µg
Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyl-CoA Thioesterase 9 antibody (ab127018)
ab127018, at 1/250 dilution, staining Acyl-CoA Thioesterase 9 in Paraffin-embedded Human Colon carcinoma tissue by Immunohistochemistry.
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