JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB252978

Anti-Acyl-CoA Thioesterase 9 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal Acyl-CoA Thioesterase 9 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ACOT9 aa 200-300.

View Alternative Names

CGI-16, ACOT9, Acyl-CoA thioesterase 9, Acyl-CoA thioester hydrolase 9

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Acyl-CoA Thioesterase 9 antibody (AB252978)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Acyl-CoA Thioesterase 9 antibody (AB252978)

PFA-fixed, Triton X-100 permeabilized U-251MG (Human brain glioma cell line) cells stained for Acyl-CoA Thioesterase 9 (green) using ab252978 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyl-CoA Thioesterase 9 antibody (AB252978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyl-CoA Thioesterase 9 antibody (AB252978)

Paraffin-embedded human bronchus tissue stained for Acyl-CoA Thioesterase 9 using ab252978 at 1/1000 dilution in immunohistochemical analysis.

Western blot - Anti-Acyl-CoA Thioesterase 9 antibody (AB252978)
  • WB

Supplier Data

Western blot - Anti-Acyl-CoA Thioesterase 9 antibody (AB252978)

All lanes:

Western blot - Anti-Acyl-CoA Thioesterase 9 antibody (ab252978) at 0.4 µg/mL

All lanes:

RH-30 cell lysate

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ACOT9 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q9Y305

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB203188

Anti-PKC nu/PRKD3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC nu/PRKD3 antibody (AB203188)

  • 0
  • 0
Primary Antibodies

AB254715

Anti-ZNF503 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF503 antibody (AB254715)

  • 0
  • 0
Primary Antibodies

AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB7291)

  • 5
  • 92
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acyl-CoA Thioesterase 9 (ACOT9) sometimes referred to as anti-CoA functions mechanically by hydrolyzing CoA esters into free fatty acids and CoA-SH playing a role in lipid metabolism. ACOT9 has a molecular mass of approximately 70 kDa. The expression of ACOT9 occurs in various tissues with higher levels observed in the liver and kidney contributing to the regulation of fatty acid and CoA concentrations within the cells.
Biological function summary

ACOT9 participates in the regulation of lipid metabolism and energy homeostasis. It does not form part of a known complex but performs a stand-alone role in converting activated fatty acids into their free form and CoA. This process contributes to the balance between synthesis and degradation of lipids impacting energy production and storage. ACOT9's action influences cellular metabolic states and can adapt them depending on the energy requirement of the organism.

Pathways

ACOT9 plays an important role in the peroxisomal and mitochondrial fatty acid oxidation pathways. In these pathways it interacts with enzymes such as Acyl-CoA oxidase and Carnitine palmitoyltransferase I facilitating the breakdown of long-chain fatty acids. ACOT9's hydrolytic activity helps reduce the build-up of acyl-CoA intermediates which is essential for maintaining cellular energy balance and supporting metabolic flexibility during fasting or exercise.

ACOT9 has been linked to metabolic syndromes including obesity and non-alcoholic fatty liver disease (NAFLD). Its modulation can influence the aberrant accumulation of lipids leading to these conditions. ACOT9's activity also connects it with proteins such as AMP-activated protein kinase (AMPK) in the regulation of energy homeostasis which is disrupted in metabolic disorders. Understanding the role of ACOT9 in these diseases may provide insights into therapeutic targets for managing metabolic imbalances.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mitochondrial acyl-CoA thioesterase. Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoA), regulating their respective intracellular levels. Regulates both mitochondrial lipid and amino acid metabolism.
See full target information ACOT9
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 10:1379 PubMed32850442

2020

Cytochrome P450 2U1 Is a Novel Independent Prognostic Biomarker in Breast Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Luo,Chuang Chen,Xiaoyan Wu,Dandan Yan,Fangfang Chen,Xinxin Yu,Jingping Yuan
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com