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AB121116

Anti-Acyl-coenzyme A Thioesterase 4 antibody

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(1 Publication)

Rabbit Polyclonal Acyl-coenzyme A Thioesterase 4 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ACOT4 aa 250 to C-terminus.

View Alternative Names

PTE2B, PTEIB, ACOT4, Peroxisomal succinyl-coenzyme A thioesterase, Acyl-coenzyme A thioesterase 4, PTE-2b, Peroxisomal acyl coenzyme A thioester hydrolase Ib, Peroxisomal long-chain acyl-CoA thioesterase Ib, Acyl-CoA thioesterase 4, PTE-Ib

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyl-coenzyme A Thioesterase 4 antibody (AB121116)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyl-coenzyme A Thioesterase 4 antibody (AB121116)

ab121116 at 1/40 dilution staining Acyl-coenzyme A Thioesterase 4 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.

Western blot - Anti-Acyl-coenzyme A Thioesterase 4 antibody (AB121116)
  • WB

Unknown

Western blot - Anti-Acyl-coenzyme A Thioesterase 4 antibody (AB121116)

All lanes:

Western blot - Anti-Acyl-coenzyme A Thioesterase 4 antibody (ab121116) at 1/250 dilution

Lane 1:

RT 4 cell lysate

Lane 2:

EFO 21 cell lysate

Lane 3:

U 138 MG cell lysate

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ACOT4 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N9L9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Acyl-coenzyme A Thioesterase 4 (ACOT4) also known as Type II acyl-CoA thioesterase is an enzyme involved in the hydrolysis of acyl-CoA thioesters to free fatty acids and CoA. ACOT4 has a molecular mass of approximately 46 kDa. It is expressed primarily in the liver and kidney tissues. This protein plays a role in lipid metabolism by regulating the intracellular levels of acyl-CoA and CoA maintaining a specific balance that is important for cellular function.
Biological function summary

The role of ACOT4 extends to fatty acid metabolism and energy homeostasis. It does not function as part of a multiprotein complex but acts directly by controlling the availability of Coenzyme A derivatives influencing cellular lipid composition. Its activity ensures proper cellular response to metabolic needs for example in situations requiring swift energy adaptations where fatty acids serve as a fuel source. The regulation of acyl-CoA levels by ACOT4 impacts various metabolic pathways by modulating the substrates available for downstream reactions.

Pathways

ACOT4 is significantly involved in the β-oxidation of fatty acids and the peroxisomal lipid metabolism. It operates in conjunction with other enzymes responsible for breaking down long-chain fatty acids particularly in the peroxisomes. ACOT4's actions closely relate to enzymes like acyl-CoA oxidase and 3-ketoacyl-CoA thiolase which are part of the peroxisomal fatty acid β-oxidation pathway. Through this interaction ACOT4 affects energy production and lipid degradation within the cells.

Abnormalities in ACOT4 expression or function have links to metabolic disorders such as obesity and type 2 diabetes. An imbalance in fatty acid metabolism can lead to excessive lipid accumulation or inadequate energy production contributing to these conditions. ACOT4's role intersects with proteins like CPT1A which is involved in the regulation of fatty acid entry into mitochondria. Mutations or dysregulation of ACOT4 may exacerbate symptoms linked to these disorders making it a target of interest in therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels (PubMed : 16940157). Functions as a peroxisomal succinyl-coenzyme A thioesterase that can also hydrolyze glutaryl-CoA and long chain saturated acyl-CoAs (PubMed : 16940157).
See full target information ACOT4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:1017120 PubMed36189307

2022

A novel immune cell signature for predicting osteosarcoma prognosis and guiding therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Runsang Pan,Feng Pan,Zhirui Zeng,Shan Lei,Yan Yang,Yushi Yang,Chujiao Hu,Houping Chen,Xiaobin Tian
View all publications
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Product promise

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