AB222913

Anti-Acyloxyacyl Hydrolase antibody

Name: Anti-Acyloxyacyl Hydrolase antibody (ab222913) | Abcam
Brand: Abcam Limited
SKU: AB222913

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(1 Publication)

Rabbit Polyclonal Acyloxyacyl Hydrolase antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AOAH aa 150-300.

View Alternative Names

Acyloxyacyl hydrolase, AOAH

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB222913)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB222913)

Paraffin-embedded human pancreatic cancer tissue stained for Acyloxyacyl Hydrolase using ab222913 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB222913)

All lanes:

Western blot - Anti-Acyloxyacyl Hydrolase antibody (ab222913) at 1/500 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 2:

Mouse skeletal muscle tissue lysate

Lane 3:

Rat lung tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 65 kDa

Observed band size: 66 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human AOAH aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

P28039

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Acyloxyacyl Hydrolase' also known as AOAH is an enzyme that cleaves secondary acyl chains from the lipid A portion of lipopolysaccharides (LPS) on bacterial membranes decreasing their toxicity. This process is known as hydrolasing activity. AOAH is a hydrolase protein with an approximate mass of 54 kDa and is widely expressed in various cells such as macrophages neutrophils and dendritic cells. Its activity is critical in modulating the immune response to LPS by removing these acyl chains which is essential in preventing excessive inflammation.

Biological function summary

Acyloxyacyl Hydrolase plays a significant role in the detoxification of LPS. As part of the host's defense mechanism AOAH works by reducing the endotoxic effects of LPS which are powerful stimulants of the immune system. Although it is not solely part of a larger protein complex its action significantly impacts the innate immune response. This enzyme ensures that the immune system does not overreact to bacterial invasion which could otherwise lead to harmful inflammatory responses.

Pathways

Acyloxyacyl Hydrolase functions within the Toll-like receptor (TLR) signaling pathway which is instrumental in recognizing LPS and initiating an immune response. AOAH modifies LPS diminishing its ability to trigger TLR4 a receptor that responds to the presence of bacterial LPS. By doing so it regulates signaling pathways involved in innate immunity. Additionally it has a relationship with proteins like myeloid differentiation factor 88 (MyD88) an important adaptor protein in the TLR signaling cascade which mediates inflammatory responses.

Acyloxyacyl Hydrolase has connections to sepsis and chronic inflammatory conditions. In sepsis the clearance of LPS by AOAH helps mitigate excessive inflammation reducing the severity of the condition. Hospitals often face challenges in managing sepsis due to overwhelming immune responses where AOAH's role in detoxifying LPS is critical. Moreover in chronic inflammatory diseases AOAH's activity serves as a checkpoint maintaining inflammation at manageable levels. Its relationship with TLR4 highlights its role in controlling pathways that drive such disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (PubMed : 1883828, PubMed : 29343645, PubMed : 8089145). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (By similarity). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (By similarity).

See full target information AOAH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:311 PubMed40801925

2025

Quercetin alleviates acute pancreatitis by modulating glycolysis and mitochondrial function via PFKFB3 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Hai Jiang,Jia Liu,Zhipeng Xu,Qi Song,Junjie Tao,Heng Zhu,Qiliang Li,Lei Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Acyloxyacyl Hydrolase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB222913)

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB222913)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise