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AB233405

Anti-Acyloxyacyl Hydrolase antibody

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(1 Publication)

Rabbit Polyclonal Acyloxyacyl Hydrolase antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AOAH aa 1-300.

View Alternative Names

Acyloxyacyl hydrolase, AOAH

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

Paraffin-embedded human glioma tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

Paraffin-embedded human liver tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

Paraffin-embedded human stomach tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

Paraffin-embedded human breast cancer tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

Paraffin-embedded human appendix tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

Paraffin-embedded human prostate cancer tissue stained for Acyloxyacyl Hydrolase using ab233405 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • WB

Supplier Data

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

All lanes:

Western blot - Anti-Acyloxyacyl Hydrolase antibody (ab233405) at 2 µg/mL

All lanes:

Recombinant human Acyloxyacyl Hydrolase protein

Predicted band size: 65 kDa

false

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • WB

Supplier Data

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

All lanes:

Western blot - Anti-Acyloxyacyl Hydrolase antibody (ab233405) at 2 µg/mL

All lanes:

Pig stomach lysate

Predicted band size: 65 kDa

false

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • WB

Supplier Data

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

All lanes:

Western blot - Anti-Acyloxyacyl Hydrolase antibody (ab233405) at 2 µg/mL

All lanes:

Human serum

Predicted band size: 65 kDa

false

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • WB

Supplier Data

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

All lanes:

Western blot - Anti-Acyloxyacyl Hydrolase antibody (ab233405) at 2 µg/mL

All lanes:

Pig spleen lysate

Predicted band size: 65 kDa

false

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB233405)
  • WB

Supplier Data

Western blot - Anti-Acyloxyacyl Hydrolase antibody (AB233405)

All lanes:

Western blot - Anti-Acyloxyacyl Hydrolase antibody (ab233405) at 2 µg/mL

All lanes:

Pig small intestine lysate

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AOAH aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P28039

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Proteins & Peptides

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YME1L1 antibody (AB234744)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-20 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-20 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab233405 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Acyloxyacyl Hydrolase' also known as AOAH is an enzyme that cleaves secondary acyl chains from the lipid A portion of lipopolysaccharides (LPS) on bacterial membranes decreasing their toxicity. This process is known as hydrolasing activity. AOAH is a hydrolase protein with an approximate mass of 54 kDa and is widely expressed in various cells such as macrophages neutrophils and dendritic cells. Its activity is critical in modulating the immune response to LPS by removing these acyl chains which is essential in preventing excessive inflammation.
Biological function summary

Acyloxyacyl Hydrolase plays a significant role in the detoxification of LPS. As part of the host's defense mechanism AOAH works by reducing the endotoxic effects of LPS which are powerful stimulants of the immune system. Although it is not solely part of a larger protein complex its action significantly impacts the innate immune response. This enzyme ensures that the immune system does not overreact to bacterial invasion which could otherwise lead to harmful inflammatory responses.

Pathways

Acyloxyacyl Hydrolase functions within the Toll-like receptor (TLR) signaling pathway which is instrumental in recognizing LPS and initiating an immune response. AOAH modifies LPS diminishing its ability to trigger TLR4 a receptor that responds to the presence of bacterial LPS. By doing so it regulates signaling pathways involved in innate immunity. Additionally it has a relationship with proteins like myeloid differentiation factor 88 (MyD88) an important adaptor protein in the TLR signaling cascade which mediates inflammatory responses.

Acyloxyacyl Hydrolase has connections to sepsis and chronic inflammatory conditions. In sepsis the clearance of LPS by AOAH helps mitigate excessive inflammation reducing the severity of the condition. Hospitals often face challenges in managing sepsis due to overwhelming immune responses where AOAH’s role in detoxifying LPS is critical. Moreover in chronic inflammatory diseases AOAH's activity serves as a checkpoint maintaining inflammation at manageable levels. Its relationship with TLR4 highlights its role in controlling pathways that drive such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (PubMed : 1883828, PubMed : 29343645, PubMed : 8089145). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (By similarity). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (By similarity).
See full target information AOAH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107891 PubMed37766980

2023

Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mick J M van Eijs,José J M Ter Linde,Matthijs J D Baars,Mojtaba Amini,Miangela M Laclé,Eelco C Brand,Eveline M Delemarre,Julia Drylewicz,Stefan Nierkens,Rik J Verheijden,Bas Oldenburg,Yvonne Vercoulen,Karijn P M Suijkerbuijk,Femke van Wijk
View all publications
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Product promise

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