AB175310

Anti-ADA antibody

Name: Anti-ADA antibody (ab175310) | Abcam
Brand: Abcam Limited
SKU: AB175310
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal ADA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ADA.

View Alternative Names

ADA1, ADA, Adenosine deaminase, Adenosine aminohydrolase

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-ADA antibody (AB175310)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ADA antibody (AB175310)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab175310. Blue DAPI for nuclear staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ADA.

P00813

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 Preservative: 0.05% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine deaminase (ADA) sometimes known as deaminase is an enzyme that plays a critical mechanical role in cellular metabolism. This enzyme catalyzes the deamination of adenosine converting it into inosine. ADA is a 41 kDa protein primarily expressed in most tissues including the thymus and spleen. Various ADA assays and ADA protocols exist to study its functionality and quantification often using ADA ELISA kits to measure ADA activity in biological samples. In cell lines such as HeLa ADA expression is also present and significant for studying its regulation and function.

Biological function summary

Adenosine deaminase activity influences purine metabolism playing an important role in the breakdown of adenosine. It is not part of a complex but interacts closely with other components of the purine salvage pathway to maintain nucleotide balance. This enzyme is important for the proper function of immune cells as it prevents toxic accumulations of adenosine which can be detrimental to lymphocyte proliferation and function. ADA's activity ensures the proper management of nucleotides within cells sustaining cellular health and functionality.

Pathways

Adenosine deaminase plays significant roles in the immune and purine catabolism pathways. Within these pathways ADA is closely related to proteins such as purine nucleoside phosphorylase (PNP) which together orchestrate the steps necessary for adenine and guanine nucleotide turnover. These proteins facilitate the conversion processes needed for cellular nucleic acid synthesis and energy regulation. By managing adenosine levels ADA's function ensures the smooth operation of cellular signaling and metabolic balance.

Adenosine deaminase deficiency is linked to severe combined immunodeficiency (SCID) and can also be associated with pulmonary disorders like asthma. A lack of ADA activity leads to the accumulation of toxic metabolites impairing immune function and contributing to the pathogenesis of SCID. In some patients with pulmonary disorders altered ADA activity impacts the inflammatory response. ADA's mechanistic interactions with other proteins such as PNP highlight its importance in maintaining immune competence and metabolic homeostasis emphasizing the enzyme's role in these clinical conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed : 16670267, PubMed : 23193172, PubMed : 26166670, PubMed : 8452534, PubMed : 9361033). Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed : 20959412). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed : 11772392). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (PubMed : 20959412). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed : 23193172). Stimulates plasminogen activation (PubMed : 15016824). Plays a role in male fertility (PubMed : 21919946, PubMed : 26166670). Plays a protective role in early postimplantation embryonic development (By similarity). Also responsible for the deamination of cordycepin (3'-deoxyadenosine), a fungal natural product that shows antitumor, antibacterial, antifungal, antivirus, and immune regulation properties (PubMed : 26038697).

See full target information ADA

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

JACC. Basic to translational science 10:101273 PubMed40866047

2025

Hypoxanthine Promotes Pulmonary Vascular Remodeling and Adenosine Deaminase Is a Therapeutic Target for Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Zhuo Shi,Yong-Jian Zhu,Meng-Jie Zhang,Yi Yan,Lu-Ling Zhao,Hong-Da Zhang,Yan Liu,Wen-Hui Wu,Zhe Cheng,Chun-Guang Qiu,Jie-Jian Kou,Yun-Feng Zhou,Xiao-Bin Pang,Ji-Wang Chen,Xin-Mei Xie,Yang-Yang He,Zhi-Cheng Jing

Nature communications 16:6861 PubMed40715150

2025

Tumour initiated purinergic signalling promotes cardiomyocyte RBFOX1 degradation and cardiotoxicity from DNA damaging anticancer agents.

Applications

Unspecified application

Species

Unspecified reactive species

Saymon Tejay,Maria Areli Lorenzana-Carrillo,Guocheng Huang,Seyed Amirhossein Tabatabaei Dakhili,Yuan -Yuan Zhao,Farah Eaton,Michelle Mendiola Pla,Dawn E Bowles,Adam Kinnaird,D Ian Paterson,Edith Pituskin,John R Ussher,Evangelos D Michelakis,Gopinath Sutendra

Cancer immunology research 11:1068-1084 PubMed37253111

2023

Immunogenetic Metabolomics Reveals Key Enzymes That Modulate CAR T-cell Metabolism and Function.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Renauer,Jonathan J Park,Meizhu Bai,Arianny Acosta,Won-Ho Lee,Guang Han Lin,Yueqi Zhang,Xiaoyun Dai,Guangchuan Wang,Youssef Errami,Terence Wu,Paul Clark,Lupeng Ye,Quanjun Yang,Sidi Chen

Molecular neurobiology 60:4396-4417 PubMed37103687

2023

Aberrant adenosine signaling in patients with focal cortical dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyi Guo,Jing Zhang,Jing Wang,Xiongfei Wang,Qing Gao,Chongyang Tang,Jiahui Deng,Zhonghua Xiong,Xiangru Kong,Yuguang Guan,Jian Zhou,Detlev Boison,Guoming Luan,Tianfu Li

Purinergic signalling : PubMed33025426

2020

Fibroblast growth factor 2 upregulates ecto-5'-nucleotidase and adenosine deaminase via MAPK pathways in cultured rat spinal cord astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ryota Eguchi,Taisuke Kitano,Ken-Ichi Otsuguro

Aging cell 19:e13218 PubMed32790148

2020

Extracellular adenosine signaling reverses the age-driven decline in the ability of neutrophils to kill Streptococcus pneumoniae.

Applications

Unspecified application

Species

Unspecified reactive species

Manmeet Bhalla,Shaunna R Simmons,Alexsandra Abamonte,Sydney E Herring,Sara E Roggensack,Elsa N Bou Ghanem

Journal of cell science 133: PubMed32079656

2020

Increased host ATP efflux and its conversion to extracellular adenosine is crucial for establishing infection.

Applications

Unspecified application

Species

Unspecified reactive species

Moumita Basu,Purnima Gupta,Ananya Dutta,Kuladip Jana,Anindita Ukil

Cellular and molecular gastroenterology and hepato 8:407-426 PubMed31195149

2019

Single-Cell Analysis Reveals Regional Reprogramming During Adaptation to Massive Small Bowel Resection in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen M Seiler,Sarah E Waye,Wenjun Kong,Kenji Kamimoto,Adam Bajinting,William H Goo,Emily J Onufer,Cathleen Courtney,Jun Guo,Brad W Warner,Samantha A Morris

Evidence-based complementary and alternative medic 2016:3632168 PubMed26941824

2016

Repeated Electroacupuncture Persistently Elevates Adenosine and Ameliorates Collagen-Induced Arthritis in Rats.

Applications

IHC

Species

Unspecified reactive species

Tian-Shen Ye,Zhong-Heng Du,Zhi-Hui Li,Wen-Xia Xie,Ka-Te Huang,Yong Chen,Zhou-Yang Chen,Huan Hu,Jun-Lu Wang,Jian-Qiao Fang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ADA antibody

Immunocytochemistry/ Immunofluorescence - Anti-ADA antibody (AB175310)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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