JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB217846

Anti-ADA antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal ADA antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ADA aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ADA1, ADA, Adenosine deaminase, Adenosine aminohydrolase

2 Images
Western blot - Anti-ADA antibody (AB217846)
  • WB

Supplier Data

Western blot - Anti-ADA antibody (AB217846)

All lanes:

Western blot - Anti-ADA antibody (ab217846) at 1/300 dilution

All lanes:

mouse spleen lysate

Secondary

All lanes:

goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/5000 dilution

Predicted band size: 41 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA antibody (AB217846)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA antibody (AB217846)

Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse embryo tissue, labeling ADA using ab217846 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ADA aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P00813

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine deaminase (ADA) sometimes known as deaminase is an enzyme that plays a critical mechanical role in cellular metabolism. This enzyme catalyzes the deamination of adenosine converting it into inosine. ADA is a 41 kDa protein primarily expressed in most tissues including the thymus and spleen. Various ADA assays and ADA protocols exist to study its functionality and quantification often using ADA ELISA kits to measure ADA activity in biological samples. In cell lines such as HeLa ADA expression is also present and significant for studying its regulation and function.
Biological function summary

Adenosine deaminase activity influences purine metabolism playing an important role in the breakdown of adenosine. It is not part of a complex but interacts closely with other components of the purine salvage pathway to maintain nucleotide balance. This enzyme is important for the proper function of immune cells as it prevents toxic accumulations of adenosine which can be detrimental to lymphocyte proliferation and function. ADA's activity ensures the proper management of nucleotides within cells sustaining cellular health and functionality.

Pathways

Adenosine deaminase plays significant roles in the immune and purine catabolism pathways. Within these pathways ADA is closely related to proteins such as purine nucleoside phosphorylase (PNP) which together orchestrate the steps necessary for adenine and guanine nucleotide turnover. These proteins facilitate the conversion processes needed for cellular nucleic acid synthesis and energy regulation. By managing adenosine levels ADA's function ensures the smooth operation of cellular signaling and metabolic balance.

Adenosine deaminase deficiency is linked to severe combined immunodeficiency (SCID) and can also be associated with pulmonary disorders like asthma. A lack of ADA activity leads to the accumulation of toxic metabolites impairing immune function and contributing to the pathogenesis of SCID. In some patients with pulmonary disorders altered ADA activity impacts the inflammatory response. ADA's mechanistic interactions with other proteins such as PNP highlight its importance in maintaining immune competence and metabolic homeostasis emphasizing the enzyme's role in these clinical conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed : 16670267, PubMed : 23193172, PubMed : 26166670, PubMed : 8452534, PubMed : 9361033). Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4 (PubMed : 20959412). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (PubMed : 11772392). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (PubMed : 20959412). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (PubMed : 23193172). Stimulates plasminogen activation (PubMed : 15016824). Plays a role in male fertility (PubMed : 21919946, PubMed : 26166670). Plays a protective role in early postimplantation embryonic development (By similarity). Also responsible for the deamination of cordycepin (3'-deoxyadenosine), a fungal natural product that shows antitumor, antibacterial, antifungal, antivirus, and immune regulation properties (PubMed : 26038697).
See full target information ADA
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22837 PubMed36934389

2023

ZNF32 prevents the activation of cancer-associated fibroblasts through negative regulation of TGFB1 transcription in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Li,Hang Yuan,Gang Zhao,Jie Zhang,Siqi Li,Di Gong,Tianyu Feng,Qiming Kou,Qijing Wang,Guanru Wang,Shan Li,Kai Li,Ping Lin

Cell metabolism 34:106-124.e10 PubMed34986329

2022

A purine metabolic checkpoint that prevents autoimmunity and autoinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Svetlana Saveljeva,Gavin W Sewell,Katharina Ramshorn,M Zaeem Cader,James A West,Simon Clare,Lea-Maxie Haag,Rodrigo Pereira de Almeida Rodrigues,Lukas W Unger,Ana Belén Iglesias-Romero,Lorraine M Holland,Christophe Bourges,Muhammad N Md-Ibrahim,James O Jones,Richard S Blumberg,James C Lee,Nicole C Kaneider,Trevor D Lawley,Allan Bradley,Gordon Dougan,Arthur Kaser
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB183631

Anti-ADA antibody

Western blot - Anti-ADA antibody (AB183631)

  • 0
  • 0
Primary Antibodies

AB175310

Anti-ADA antibody

Immunocytochemistry/ Immunofluorescence - Anti-ADA antibody (AB175310)

  • 4
  • 1
Primary Antibodies

AB108352

Anti-ADA antibody [EPR4438]

RabMAb|Recombinant

Western blot - Anti-ADA antibody [EPR4438] (AB108352)

  • 0
  • 0
Primary Antibodies

AB226395

Anti-ADA antibody

Immunoprecipitation - Anti-ADA antibody (AB226395)

  • 0
  • 0

Complementary Products

Assay Kits

AB211093

Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric)

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Colorimetric) (AB211093)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB204695

Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric)

Functional Studies - Adenosine Deaminase (ADA) Activity Assay Kit (Fluorometric) (AB204695)

  • 4
  • 1
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com