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AB215524

Anti-ADA2 antibody

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(1 Publication)

Rabbit Polyclonal CECR1 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ADA2.

View Alternative Names

YDR448W, D9461.33, ADA2, Transcriptional adapter 2

1 Images
Western blot - Anti-ADA2 antibody (AB215524)
  • WB

Supplier Data

Western blot - Anti-ADA2 antibody (AB215524)

All lanes:

Western blot - Anti-ADA2 antibody (ab215524) at 1/500 dilution

All lanes:

S. cerevisiae whole cell extract

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ADA2.

Q02336

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Reactivity data

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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.1% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine deaminase 2 (ADA2) also known as ADA002 ADA-DUA or ADA2 is a protein involved in immune regulation and inflammation. ADA2 has a molecular mass of approximately 59 kDa. This enzyme expresses mainly in monocytes macrophages and dendritic cells. Its function involves the conversion of adenosine to inosine a critical step in purine metabolism that impacts cell signaling and immune responses.
Biological function summary

ADA2 plays an important role in modulating immune system activity and inflammation. Unlike its counterpart ADA1 ADA2 does not function as a monomer but as part of a larger protein complex. It participates in the maturation and differentiation of monocytes into macrophages and dendritic cells influencing both innate and adaptive immune responses. ADA2's functions suggest it has an effect on the vascular and immune system health.

Pathways

ADA2 holds a significant place in the adenosine signaling pathway where it regulates the balance between adenosine and inosine levels. This balance affects the extracellular signaling pathways that influence immune cell activity. ADA2 also interacts with other proteins in the inflammatory pathways notably influencing cytokine activity. Through these pathways it shows regulatory connections with proteins like CD39 and CD73 which also engage in purinergic signaling.

ADA2 plays an essential role in pathologies involving inflammation and immune dysregulation. Deficiency in ADA2 links to rare genetic disorders such as deficiency of ADA2 (DADA2) which presents with systemic inflammation vasculopathy and hematological abnormalities. In addition ADA2 shows involvement in conditions like rheumatoid arthritis where inflammation is a central feature. Its interaction with TNF-alpha and other pro-inflammatory cytokines positions ADA2 as a potential therapeutic target in inflammation-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and ADA. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. ADA preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B.
See full target information ADA2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 12:705012 PubMed34335537

2021

Light-Stress Response Mediated by the Transcription Factor Mga2 in the Yeast .

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Camponeschi,Arianna Montanari,Marzia Beccaccioli,Massimo Reverberi,Cristina Mazzoni,Michele M Bianchi
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