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Rabbit Recombinant Monoclonal CECR1 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] (AB288296), expandable thumbnail
  • Western blot - Anti-ADA2 antibody [EPR25430-131] (AB288296), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ADA2 antibody [EPR25430-131] (AB288296), expandable thumbnail
  • Western blot - Anti-ADA2 antibody [EPR25430-131] (AB288296), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] (AB288296), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBIHC-PICC/IFIP
Human
Tested
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Adenosine deaminase that may contribute to the degradation of extracellular adenosine, a signaling molecule that controls a variety of cellular responses. Requires elevated adenosine levels for optimal enzyme activity. Binds to cell surfaces via proteoglycans and may play a role in the regulation of cell proliferation and differentiation, independently of its enzyme activity.

Alternative names

ADGF, CECR1, IDGFL, ADA2, Adenosine deaminase 2, Cat eye syndrome critical region protein 1

Recommended products

Rabbit Recombinant Monoclonal CECR1 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25430-131
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Adenosine deaminase 2 (ADA2) also known as ADA002 ADA-DUA or ADA2 is a protein involved in immune regulation and inflammation. ADA2 has a molecular mass of approximately 59 kDa. This enzyme expresses mainly in monocytes macrophages and dendritic cells. Its function involves the conversion of adenosine to inosine a critical step in purine metabolism that impacts cell signaling and immune responses.

Biological function summary

ADA2 plays an important role in modulating immune system activity and inflammation. Unlike its counterpart ADA1 ADA2 does not function as a monomer but as part of a larger protein complex. It participates in the maturation and differentiation of monocytes into macrophages and dendritic cells influencing both innate and adaptive immune responses. ADA2's functions suggest it has an effect on the vascular and immune system health.

Pathways

ADA2 holds a significant place in the adenosine signaling pathway where it regulates the balance between adenosine and inosine levels. This balance affects the extracellular signaling pathways that influence immune cell activity. ADA2 also interacts with other proteins in the inflammatory pathways notably influencing cytokine activity. Through these pathways it shows regulatory connections with proteins like CD39 and CD73 which also engage in purinergic signaling.

Associated diseases and disorders

ADA2 plays an essential role in pathologies involving inflammation and immune dysregulation. Deficiency in ADA2 links to rare genetic disorders such as deficiency of ADA2 (DADA2) which presents with systemic inflammation vasculopathy and hematological abnormalities. In addition ADA2 shows involvement in conditions like rheumatoid arthritis where inflammation is a central feature. Its interaction with TNF-alpha and other pro-inflammatory cytokines positions ADA2 as a potential therapeutic target in inflammation-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] (ab288296), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] (ab288296)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling ADA2 with ab288296 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Cytoplasmic staining on human tonsil. The section was incubated with ab288296 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

    Antigen Retrieval: Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-ADA2 antibody [EPR25430-131] (ab288296), expandable thumbnail

    Western blot - Anti-ADA2 antibody [EPR25430-131] (ab288296)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: HUVEC(PMID: 24552284, 32743071)

    Exposure time: 5.5 seconds

    All lanes: Western blot - Anti-ADA2 antibody [EPR25430-131] (ab288296) at 1/1000 dilution

    Lane 1: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

    Lane 2: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 3: U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Lane 4: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

  • Flow Cytometry (Intracellular) - Anti-ADA2 antibody [EPR25430-131] (ab288296), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ADA2 antibody [EPR25430-131] (ab288296)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HUVEC (human umbilical vein endothelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling ADA2 with ab288296 at 1/50 dilution (1μg)(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

    Low expression: HUVEC (PMID: 24552284, 32743071).

  • Western blot - Anti-ADA2 antibody [EPR25430-131] (ab288296), expandable thumbnail

    Western blot - Anti-ADA2 antibody [EPR25430-131] (ab288296)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1-2: 70 seconds; Lane 3: 7.75 seconds

    All lanes: Western blot - Anti-ADA2 antibody [EPR25430-131] (ab288296) at 1/1000 dilution

    Lane 1: Human plasma at 20 µL

    Lane 2: Human heart tissue lysate at 20 µg

    Lane 3: Human lung tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] (ab288296), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] (ab288296)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling ADA2 with ab288296 (0.259 μg/ml) at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Cytoplasmic staining on human colon. The section was incubated with ab288296 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.


    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

    Antigen Retrieval: Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] (ab288296), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] (ab288296)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling ADA2 with ab288296 (0.259 μg/ml) at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Negative control: No staining on human skeletal muscle. The section was incubated with ab288296 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection) was used.

    Antigen Retrieval: Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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