Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305)

This data was developed using 288296, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling ADA2 with ab288296 (0.259 μg/ml) at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Negative control : No staining on human skeletal muscle. The section was incubated with ab288296 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection) was used.

Antigen Retrieval : Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305)

This data was developed using 288296, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling ADA2 with ab288296 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Cytoplasmic staining on human tonsil. The section was incubated with ab288296 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

Antigen Retrieval : Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305)

This data was developed using 288296, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling ADA2 with ab288296 (0.259 μg/ml) at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond^®, Polymer Refine Detection). Cytoplasmic staining on human colon. The section was incubated with ab288296 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

Antigen Retrieval : Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305)

This data was developed using 288296, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HUVEC (human umbilical vein endothelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling ADA2 with ab288296 at 1/50 dilution (1μg)(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Low expression : HUVEC (PMID : 24552284, 32743071).

• WB

Supplier Data

Western blot - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305)

This data was developed using 288296, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane 1-2 : 70 seconds; Lane 3 : 7.75 seconds

All lanes:

Western blot - Anti-ADA2 antibody [EPR25430-131] (<a href='/en-us/products/primary-antibodies/ada2-antibody-epr25430-131-ab288296'>ab288296</a>) at 1/1000 dilution

Lane 1:

Human plasma at 20 µL

Lane 2:

Human heart tissue lysate at 20 µg

Lane 3:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false

• WB

Supplier Data

Western blot - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305)

This data was developed using 288296, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Low expression : HUVEC(PMID : 24552284, 32743071)

Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-ADA2 antibody [EPR25430-131] (<a href='/en-us/products/primary-antibodies/ada2-antibody-epr25430-131-ab288296'>ab288296</a>) at 1/1000 dilution

Lane 1:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 3:

U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 4:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 58 kDa

Observed band size: 58 kDa

false