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Rabbit Recombinant Monoclonal CECR1 antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P, WB and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305), expandable thumbnail
  • Western blot - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305), expandable thumbnail
  • Western blot - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (AB288305), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-PWBICC/IFIP
Human
Tested
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Target data

Function

Adenosine deaminase that may contribute to the degradation of extracellular adenosine, a signaling molecule that controls a variety of cellular responses. Requires elevated adenosine levels for optimal enzyme activity. Binds to cell surfaces via proteoglycans and may play a role in the regulation of cell proliferation and differentiation, independently of its enzyme activity.

Alternative names

Adenosine deaminase 2, Cat eye syndrome critical region protein 1, ADA2, IDGFL, CECR1, ADGF

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Rabbit Recombinant Monoclonal CECR1 antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P, WB and reacts with Human samples.

Alternative names

Adenosine deaminase 2, Cat eye syndrome critical region protein 1, ADA2, IDGFL, CECR1, ADGF

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR25430-131

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Activity summary

Adenosine deaminase 2 (ADA2) also known as ADA002 ADA-DUA or ADA2 is a protein involved in immune regulation and inflammation. ADA2 has a molecular mass of approximately 59 kDa. This enzyme expresses mainly in monocytes macrophages and dendritic cells. Its function involves the conversion of adenosine to inosine a critical step in purine metabolism that impacts cell signaling and immune responses.

Biological function summary

ADA2 plays an important role in modulating immune system activity and inflammation. Unlike its counterpart ADA1 ADA2 does not function as a monomer but as part of a larger protein complex. It participates in the maturation and differentiation of monocytes into macrophages and dendritic cells influencing both innate and adaptive immune responses. ADA2's functions suggest it has an effect on the vascular and immune system health.

Pathways

ADA2 holds a significant place in the adenosine signaling pathway where it regulates the balance between adenosine and inosine levels. This balance affects the extracellular signaling pathways that influence immune cell activity. ADA2 also interacts with other proteins in the inflammatory pathways notably influencing cytokine activity. Through these pathways it shows regulatory connections with proteins like CD39 and CD73 which also engage in purinergic signaling.

Associated diseases and disorders

ADA2 plays an essential role in pathologies involving inflammation and immune dysregulation. Deficiency in ADA2 links to rare genetic disorders such as deficiency of ADA2 (DADA2) which presents with systemic inflammation vasculopathy and hematological abnormalities. In addition ADA2 shows involvement in conditions like rheumatoid arthritis where inflammation is a central feature. Its interaction with TNF-alpha and other pro-inflammatory cytokines positions ADA2 as a potential therapeutic target in inflammation-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305)

    This data was developed using 288296, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling ADA2 with Anti-ADA2 antibody [EPR25430-131] ab288296 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Cytoplasmic staining on human tonsil. The section was incubated with Anti-ADA2 antibody [EPR25430-131] ab288296 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

    Antigen Retrieval: Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305), expandable thumbnail

    Western blot - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305)

    This data was developed using 288296, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: HUVEC(PMID: 24552284, 32743071)

    Exposure time: 5.5 seconds

    All lanes: Western blot - Anti-ADA2 antibody [EPR25430-131] (Anti-ADA2 antibody [EPR25430-131] ab288296) at 1/1000 dilution

    Lane 1: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

    Lane 2: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 3: U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Lane 4: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

  • Flow Cytometry (Intracellular) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305)

    This data was developed using 288296, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HUVEC (human umbilical vein endothelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling ADA2 with Anti-ADA2 antibody [EPR25430-131] ab288296 at 1/50 dilution (1μg)(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

    Low expression: HUVEC (PMID: 24552284, 32743071).

  • Western blot - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305), expandable thumbnail

    Western blot - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305)

    This data was developed using 288296, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: Lane 1-2: 70 seconds; Lane 3: 7.75 seconds

    All lanes: Western blot - Anti-ADA2 antibody [EPR25430-131] (Anti-ADA2 antibody [EPR25430-131] ab288296) at 1/1000 dilution

    Lane 1: Human plasma at 20 µL

    Lane 2: Human heart tissue lysate at 20 µg

    Lane 3: Human lung tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 58 kDa

    Observed band size: 58 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305)

    This data was developed using 288296, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling ADA2 with Anti-ADA2 antibody [EPR25430-131] ab288296 (0.259 μg/ml) at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Cytoplasmic staining on human colon. The section was incubated with Anti-ADA2 antibody [EPR25430-131] ab288296 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection).

    Antigen Retrieval: Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADA2 antibody [EPR25430-131] - BSA and Azide free (ab288305)

    This data was developed using 288296, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling ADA2 with Anti-ADA2 antibody [EPR25430-131] ab288296 (0.259 μg/ml) at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection). Negative control: No staining on human skeletal muscle. The section was incubated with Anti-ADA2 antibody [EPR25430-131] ab288296 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond®, Polymer Refine Detection) was used.

    Antigen Retrieval: Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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