Anti-ADAM10 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody (AB1997)

Immunofluorescence of ADAM10 in K562 cells using ab1997 at 10 ug/ml.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ADAM10 antibody (AB1997)

ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC

Unknown

Immunocytochemistry - Anti-ADAM10 antibody (AB1997)

ab1997 at 2µg/ml staining K562 cells by ICC/IF.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM10 antibody (AB1997)

Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-ADAM10 antibody (ab1997) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-ADAM10 antibody (AB1997)

1 h incubation at RT in 5% NFDM/TBST

All lanes:

Western blot - Anti-ADAM10 antibody (ab1997)

Lane 1:

WT 293T Cells at 10 µg

Lane 2:

ADAM10 knockout 293T Cells at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 84 kDa

Observed band size: 94 kDa,68 kDa

false

• WB

Supplier Data

Western blot - Anti-ADAM10 antibody (AB1997)

All lanes:

Western blot - Anti-ADAM10 antibody (ab1997) at 1 µg/mL

Lane 1:

Mouse pancreas at 15 µg

Lane 2:

Mouse stomach at 15 µg

Lane 3:

Mouse testis at 15 µg

Lane 4:

K562 cell lysates at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 84 kDa

Observed band size: 94 kDa,68 kDa

false

• WB

AbReview4881****

Western blot - Anti-ADAM10 antibody (AB1997)

All lanes:

Western blot - Anti-ADAM10 antibody (ab1997) at 1/1666 dilution

Lane 2:

ADAM10 siRNA treated human keratinocyte cells

Lane 3:

Non-treated human keratinocyte cells.

Secondary

All lanes:

HRP conjugated Goat anti-rabbit antibody

Predicted band size: 84 kDa

Observed band size: 100 kDa,75 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

• WB

Supplier Data

Western blot - Anti-ADAM10 antibody (AB1997)

All lanes:

Western blot - Anti-ADAM10 antibody (ab1997) at 1 µg/mL

Lane 1:

A549 cell lysates at 15 µg

Lane 2:

Caco2 cell lysates at 15 µg

Lane 3:

Daudi cell lysates at 15 µg

Lane 4:

HeLa cell lysates at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 84 kDa

Observed band size: 94 kDa,68 kDa

false

• WB

Supplier Data

Western blot - Anti-ADAM10 antibody (AB1997)

All lanes:

Western blot - Anti-ADAM10 antibody (ab1997) at 1 µg/mL

Lane 1:

Rat Kidney at 15 µg

Lane 2:

Rat Thymus at 15 µg

Lane 3:

Rat Pancreas at 15 µg

Lane 4:

K562 cell lysates at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 84 kDa

Observed band size: 94 kDa,68 kDa

false

• WB

Supplier Data

Western blot - Anti-ADAM10 antibody (AB1997)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-ADAM10 antibody (ab1997) at 10 µg

Lane 1:

WT Mouse MEF Cells at 10 µg

Lane 2:

ADAM10 knockout Mouse MEF Cells at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 84 kDa

Observed band size: 94 kDa,68 kDa

false

• WB

AbReview44044****

Western blot - Anti-ADAM10 antibody (AB1997)

All lanes:

Western blot - Anti-ADAM10 antibody (ab1997) at 1/1000 dilution

All lanes:

Human frontal cortex tissue lysate - whole at 20 µg

Secondary

All lanes:

IRDye® 800CW-conjugated donkey anti-rabbit IgG polyclonal at 1/18000 dilution

Predicted band size: 84 kDa

Observed band size: 65 kDa,80 kDa

false

Exposure time: 330s

This image is courtesy of an Abreview submitted by Grant Corbett