AB316805

Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (Detector)

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal ADAM10 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CD156c, KUZ, MADM, ADAM10, Disintegrin and metalloproteinase domain-containing protein 10, ADAM 10, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease

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Sandwich ELISA - Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (Detector) (AB316805)

sELISA

Supplier Data

Sandwich ELISA - Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (Detector) (AB316805)

Sandwich ELISA of ab316803 with the capture antibody ab316804 dilution at 2 ug/mL and detector antibody ab316805 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23491-74

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab316805 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ADAM10. The recommended pair for sandwich ELISA is:

Capture: ab316804, Human ADAM10 Capture Antibody (unconjugated)

Detector: ab316805, Human ADAM10 Detector Antibody (unconjugated)

The reference range value is 31.25-4000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ADAM10 protein also known as CD156c is a member of the ADAM (a disintegrin and metalloprotease) family. It functions as a metalloproteinase enzyme involved in the ectodomain shedding of various cell surface proteins. ADAM10 has an approximate molecular weight of 85 kDa. This protein has broad expression in various tissues including the brain heart and liver and performs key enzymatic activities that affect cellular communication.

Biological function summary

ADAM10 contributes to development and regulation of synapses in the central nervous system being part of the membrane protein complex. It processes amyloid precursor protein (APP) and other substrates excising their extracellular domains and influencing cell signaling pathways. ADAM10 activity regulates Notch signaling a mechanism essential for cell differentiation and tissue homeostasis.

Pathways

ADAM10 activity intersects with several important biological processes including the Notch and Epidermal Growth Factor Receptor (EGFR) pathways. Notch signaling pathway involves interactions with ligands like Jagged and Delta impacting cell fate decisions. In the EGFR pathway ADAM10 regulates the availability of ligands such as EGF affecting the receptor's signaling cascades related to cell proliferation and survival.

ADAM10 dysregulation associates with Alzheimer's disease and cancer. ADAM10's role in cleaving APP is linked to Alzheimer's where altered activity may affect amyloid-beta production and plaque formation. In cancer ADAM10 modulates cell adhesion and migration influencing tumor progression and metastasis. ADAM10 interactions with proteins in these contexts such as β-Catenin in cancer illustrate its impact on pathophysiological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins, including adhesion proteins, growth factor precursors and cytokines being essential for development and tissue homeostasis (PubMed : 11786905, PubMed : 12475894, PubMed : 20592283, PubMed : 24990881, PubMed : 26686862, PubMed : 28600292, PubMed : 31792032). Associates with six members of the tetraspanin superfamily TspanC8 which regulate its exit from the endoplasmic reticulum and its substrate selectivity (PubMed : 26686862, PubMed : 28600292, PubMed : 31792032, PubMed : 34739841, PubMed : 37516108). Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed : 20592283). Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) (PubMed : 11786905, PubMed : 26686862, PubMed : 29224781, PubMed : 34739841). Contributes to the normal cleavage of the cellular prion protein (PubMed : 11477090). Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (PubMed : 12475894). Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity). Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (PubMed : 17557115). Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (PubMed : 19114711, PubMed : 21288900). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity). Mediates the proteolytic cleavage of IL6R and IL11RA, leading to the release of secreted forms of IL6R and IL11RA (PubMed : 26876177). Enhances the cleavage of CHL1 by BACE1 (By similarity). Cleaves NRCAM (By similarity). Cleaves TREM2, resulting in shedding of the TREM2 ectodomain (PubMed : 24990881). Involved in the development and maturation of glomerular and coronary vasculature (By similarity). During development of the cochlear organ of Corti, promotes pillar cell separation by forming a ternary complex with CADH1 and EPHA4 and cleaving CADH1 at adherens junctions (By similarity). May regulate the EFNA5-EPHA3 signaling (PubMed : 16239146). Regulates leukocyte transmigration as a sheddase for the adherens junction protein VE-cadherin/CDH5 in endothelial cells (PubMed : 28600292).. (Microbial infection) Promotes the cytotoxic activity of S.aureus hly by binding to the toxin at zonula adherens and promoting formation of toxin pores.

See full target information ADAM10

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Product promise

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Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-ADAM10 antibody [EPR23491-74] - BSA and Azide free (Detector) (AB316805)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise