Name: Anti-ADAM10 antibody [EPR5622]
SKU: ab124695
Rating: 4 (4 reviews)

Anti-ADAM10 antibody [EPR5622] (ab124695) is a rabbit monoclonal antibody that is used to detect ADAM10 in Western Blot, IP. Suitable for Human, Mouse, Rat samples.

- Specificity confirmed with ADAM10 knockout cell line validation

Images

Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (AB124695), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB
Human	Not recommended	Tested	Tested
Mouse	Not recommended	Expected	Tested
Rat	Not recommended	Expected	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000 - 1/10000	Notes -
Species Rat	Dilution info 1/1000 - 1/10000	Notes -
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Associated Products

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3 products for Alternative Product

Target data

See full target information ADAM10

Function

Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins, including adhesion proteins, growth factor precursors and cytokines being essential for development and tissue homeostasis (PubMed:11786905, PubMed:12475894, PubMed:20592283, PubMed:24990881, PubMed:26686862, PubMed:28600292, PubMed:31792032). Associates with six members of the tetraspanin superfamily TspanC8 which regulate its exit from the endoplasmic reticulum and its substrate selectivity (PubMed:26686862, PubMed:28600292, PubMed:31792032, PubMed:34739841, PubMed:37516108). Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) (PubMed:11786905, PubMed:26686862, PubMed:29224781, PubMed:34739841). Contributes to the normal cleavage of the cellular prion protein (PubMed:11477090). Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (PubMed:12475894). Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity). Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (PubMed:17557115). Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (PubMed:19114711, PubMed:21288900). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity). Mediates the proteolytic cleavage of IL6R and IL11RA, leading to the release of secreted forms of IL6R and IL11RA (PubMed:26876177). Enhances the cleavage of CHL1 by BACE1 (By similarity). Cleaves NRCAM (By similarity). Cleaves TREM2, resulting in shedding of the TREM2 ectodomain (PubMed:24990881). Involved in the development and maturation of glomerular and coronary vasculature (By similarity). During development of the cochlear organ of Corti, promotes pillar cell separation by forming a ternary complex with CADH1 and EPHA4 and cleaving CADH1 at adherens junctions (By similarity). May regulate the EFNA5-EPHA3 signaling (PubMed:16239146). Regulates leukocyte transmigration as a sheddase for the adherens junction protein VE-cadherin/CDH5 in endothelial cells (PubMed:28600292). (Microbial infection) Promotes the cytotoxic activity of S.aureus hly by binding to the toxin at zonula adherens and promoting formation of toxin pores.

Alternative names

CD156c, KUZ, MADM, ADAM10, Disintegrin and metalloproteinase domain-containing protein 10, ADAM 10, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR5622
Purification technique: Affinity purification Protein A
Dissociation constant: 1.8 x 10^-10 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Notes

What is this antibody validated in?

Anti-ADAM10 antibody [EPR5622] (ab124695) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human, Mouse, Rat samples.

What is the molecular weight of ADAM10?

Anti-ADAM10 [EPR5622] (ab124695) specifically detects a band for ADAM10 (UniProt: O14672) at a molecular weight of 84kDa.

Trusted by the scientific community

Anti-ADAM10 [EPR5622] (ab124695) was first used in a scientific publication in 2012 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-ADAM10 antibody [EPR5622] (ab124695) has been confirmed by Western blot testing in ADAM10 Knockout HAP1 cells.

Other related products

We have a range of other formats of antibody clone [EPR5622] also available for your convenience:
ab124695, Carrier free - ab242389

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ADAM10 protein also known as CD156c is a member of the ADAM (a disintegrin and metalloprotease) family. It functions as a metalloproteinase enzyme involved in the ectodomain shedding of various cell surface proteins. ADAM10 has an approximate molecular weight of 85 kDa. This protein has broad expression in various tissues including the brain heart and liver and performs key enzymatic activities that affect cellular communication.

Biological function summary

ADAM10 contributes to development and regulation of synapses in the central nervous system being part of the membrane protein complex. It processes amyloid precursor protein (APP) and other substrates excising their extracellular domains and influencing cell signaling pathways. ADAM10 activity regulates Notch signaling a mechanism essential for cell differentiation and tissue homeostasis.

Pathways

ADAM10 activity intersects with several important biological processes including the Notch and Epidermal Growth Factor Receptor (EGFR) pathways. Notch signaling pathway involves interactions with ligands like Jagged and Delta impacting cell fate decisions. In the EGFR pathway ADAM10 regulates the availability of ligands such as EGF affecting the receptor’s signaling cascades related to cell proliferation and survival.

Associated diseases and disorders

ADAM10 dysregulation associates with Alzheimer’s disease and cancer. ADAM10's role in cleaving APP is linked to Alzheimer's where altered activity may affect amyloid-beta production and plaque formation. In cancer ADAM10 modulates cell adhesion and migration influencing tumor progression and metastasis. ADAM10 interactions with proteins in these contexts such as Β-Catenin in cancer illustrate its impact on pathophysiological processes.

Product promise

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7 product images

Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)
ab124695 (purified) at 1:30 dilution (2ug) immunoprecipitating ADAM-10 in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug.
Lane 1 (input): LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab124695 & LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ASS1 antibody [EPR12398] ab170952 in HeLa LNCaP (Human prostate carcinoma epithelial cell) whole cell lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)
Predicted band size: 84 kDa
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
Lane 1: Wild type HAP1 whole cell lysate (40 μg)
Lane 2: Empty lane
Lane 3: ADAM10 knockout HAP1 whole cell lysate (40 μg)
Lane 4: U20S whole cell lysate (40 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124695 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab124695 was shown to recognize ADAM10 when ADAM10 knockout samples were used, along with additional cross-reactive bands. Wild-type and ADAM10 knockout samples were subjected to SDS-PAGE. ab124695 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
Predicted band size: 84 kDa
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695) at 0.05 µg/mL
Lane 1: RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: Rat brain lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 84 kDa
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695) at 0.09 µg/mL
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Lane 2: MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 21 kDa, 84 kDa
Observed band size: 19 kDa
OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] (ab124695)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
84 kDa band is pro-ADAM10, smaller band at around 65 kDa is mature ADAM10 protein.
All lanes: Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: RAW 264.7 cell lysate at 10 µg
Lane 3: LnCaP cell lysate at 10 µg
Lane 4: MCF-7 cell lysate at 10 µg
Predicted band size: 84 kDa
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)
False colour image of Western blot: Anti-ADAM10 antibody [EPR5622] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124695 was shown to bind specifically to ADAM10. A band was observed at 95/120 kDa in wild-type Jurkat cell lysates with no signal observed at this size in ADAM10 knockout cell line. The band at 120 kDa is likely to be the precursor and the band at 90 kDa is likely to be the active form of ADAM10. To generate this image, wild-type and ADAM10 knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695) at 1/1000 dilution
Lane 1: Wild-type Jurkat cell lysate at 10 µg
Lane 2: ADAM10 knockout Jurkat cell lysate at 10 µg
Lane 3: MCF7 cell lysate at 20 µg
Lane 4: U-2 OS cell lysate at 20 µg
Performed under reducing conditions.
Observed band size: 95-120 kDa

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Anti-ADAM10 antibody [EPR5622]

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