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Rabbit Recombinant Monoclonal ADAM10 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (AB242389), expandable thumbnail
  • Western blot - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (AB242389), expandable thumbnail
  • OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (AB242389), expandable thumbnail
  • Western blot - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (AB242389), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) (PubMed:26686862, PubMed:11786905, PubMed:29224781). Contributes to the normal cleavage of the cellular prion protein (PubMed:11477090). Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (PubMed:12475894). Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity). Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (PubMed:17557115). Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (PubMed:19114711, PubMed:21288900). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity). Mediates the proteolytic cleavage of IL6R and IL11RA, leading to the release of secreted forms of IL6R and IL11RA (PubMed:26876177). Enhances the cleavage of CHL1 by BACE1 (By similarity). Cleaves NRCAM (By similarity). Cleaves TREM2, resulting in shedding of the TREM2 ectodomain (PubMed:24990881). Involved in the development and maturation of glomerular and coronary vasculature (By similarity). During development of the cochlear organ of Corti, promotes pillar cell separation by forming a ternary complex with CADH1 and EPHA4 and cleaving CADH1 at adherens junctions (By similarity). May regulate the EFNA5-EPHA3 signaling (PubMed:16239146).(Microbial infection) Promotes the cytotoxic activity of S.aureus hly by binding to the toxin at zonula adherens and promoting formation of toxin pores.

Alternative names

Disintegrin and metalloproteinase domain-containing protein 10, ADAM 10, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease, ADAM10, KUZ, MADM

Recommended products

Rabbit Recombinant Monoclonal ADAM10 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

Alternative names

Disintegrin and metalloproteinase domain-containing protein 10, ADAM 10, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease, ADAM10, KUZ, MADM

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR5622

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab242389 is the carrier-free version of Anti-ADAM10 antibody [EPR5622] ab124695.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ADAM10 protein also known as CD156c is a member of the ADAM (a disintegrin and metalloprotease) family. It functions as a metalloproteinase enzyme involved in the ectodomain shedding of various cell surface proteins. ADAM10 has an approximate molecular weight of 85 kDa. This protein has broad expression in various tissues including the brain heart and liver and performs key enzymatic activities that affect cellular communication.

Biological function summary

ADAM10 contributes to development and regulation of synapses in the central nervous system being part of the membrane protein complex. It processes amyloid precursor protein (APP) and other substrates excising their extracellular domains and influencing cell signaling pathways. ADAM10 activity regulates Notch signaling a mechanism essential for cell differentiation and tissue homeostasis.

Pathways

ADAM10 activity intersects with several important biological processes including the Notch and Epidermal Growth Factor Receptor (EGFR) pathways. Notch signaling pathway involves interactions with ligands like Jagged and Delta impacting cell fate decisions. In the EGFR pathway ADAM10 regulates the availability of ligands such as EGF affecting the receptor’s signaling cascades related to cell proliferation and survival.

Associated diseases and disorders

ADAM10 dysregulation associates with Alzheimer’s disease and cancer. ADAM10's role in cleaving APP is linked to Alzheimer's where altered activity may affect amyloid-beta production and plaque formation. In cancer ADAM10 modulates cell adhesion and migration influencing tumor progression and metastasis. ADAM10 interactions with proteins in these contexts such as β-Catenin in cancer illustrate its impact on pathophysiological processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389), expandable thumbnail

    Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)

    Anti-ADAM10 antibody [EPR5622] ab124695 (purified) at 1:30 dilution (2ug) immunoprecipitating ADAM-10 in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug.
    Lane 1 (input): LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): Anti-ADAM10 antibody [EPR5622] ab124695 & LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ASS1 antibody [EPR12398] ab170952 in HeLa LNCaP (Human prostate carcinoma epithelial cell) whole cell lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ADAM10 antibody [EPR5622] ab124695).

    All lanes: Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (Anti-ADAM10 antibody [EPR5622] ab124695)

    Predicted band size: 84 kDa

  • Western blot - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389), expandable thumbnail

    Western blot - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)

    Lane 1: Wild type HAP1 whole cell lysate (40 μg)
    Lane 2: Empty lane
    Lane 3: ADAM10 knockout HAP1 whole cell lysate (40 μg)
    Lane 4: U20S whole cell lysate (40 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-ADAM10 antibody [EPR5622] ab124695 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-ADAM10 antibody [EPR5622] ab124695 was shown to recognize ADAM10 when ADAM10 knockout samples were used, along with additional cross-reactive bands. Wild-type and ADAM10 knockout samples were subjected to SDS-PAGE. Anti-ADAM10 antibody [EPR5622] ab124695 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ADAM10 antibody [EPR5622] ab124695).

    All lanes: Western blot - Anti-ADAM10 antibody [EPR5622] (Anti-ADAM10 antibody [EPR5622] ab124695)

    Predicted band size: 84 kDa

  • OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389), expandable thumbnail

    OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389), expandable thumbnail

    Western blot - Anti-ADAM10 antibody [EPR5622] - BSA and Azide free (ab242389)

    False colour image of Western blot: Anti-ADAM10 antibody [EPR5622] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-ADAM10 antibody [EPR5622] ab124695 was shown to bind specifically to ADAM10. A band was observed at 95/120 kDa in wild-type Jurkat cell lysates with no signal observed at this size in ADAM10 knockout cell line. The band at 120 kDa is likely to be the precursor and the band at 90 kDa is likely to be the active form of ADAM10. To generate this image, wild-type and ADAM10 knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-ADAM10 antibody [EPR5622] (Anti-ADAM10 antibody [EPR5622] ab124695) at 1/1000 dilution

    Lane 1: Wild-type Jurkat cell lysate at 10 µg

    Lane 2: ADAM10 knockout Jurkat cell lysate at 10 µg

    Lane 3: MCF7 cell lysate at 20 µg

    Lane 4: U-2 OS cell lysate at 20 µg

    Performed under reducing conditions.

    Observed band size: 95-120 kDa

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