AB223476

Anti-ADAM12 antibody

Name: Anti-ADAM12 antibody (ab223476) | Abcam
Brand: Abcam Limited
SKU: AB223476
Price: 525 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal ADAM12 antibody. Suitable for WB and reacts with Mouse, Rat, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ADAM12 aa 200-250.

View Alternative Names

MLTN, UNQ346/PRO545, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, ADAM 12, Meltrin-alpha

2 Images

Supplier Data

Western blot - Anti-ADAM12 antibody (AB223476)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ADAM12 antibody (ab223476) at 0.1 µg/mL

All lanes:

Human heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 100 kDa

Observed band size: 100 kDa

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Supplier Data

Western blot - Anti-ADAM12 antibody (AB223476)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-ADAM12 antibody (ab223476) at 0.1 µg/mL

Lane 1:

Mouse heart lysate (in RIPA buffer) at 35 µg

Lane 2:

Rat heart lysate (in RIPA buffer) at 35 µg

Lane 3:

Pig heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 100 kDa

Observed band size: 60 kDa,90 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

applications

Immunogen

Synthetic Peptide within Human ADAM12 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

O43184

Specificity

ab223476 is expected to recognize both reported isoforms (NP_003465.3; NP_067673.2).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab223476 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAM12 also known as meltrin-alpha is a member of the ADAM (a disintegrin and metalloproteinase) family and has a mass of approximately 110 kDa. It functions primarily in the modulation of cell-cell and cell-matrix interactions due to its metalloprotease and disintegrin domains. ADAM12 protein is expressed in various tissues including muscle and placenta. Although found in many cell types it is particularly abundant during embryogenesis and development reflecting its role in these processes.

Biological function summary

The ADAM12 protein holds a role in cell signaling migration and proliferation. It is often part of complexes that interact with other extracellular matrix components influencing cell adhesion and motility. Through its protease activity ADAM12 facilitates growth factor signaling by cleaving membrane-bound precursor proteins to release active growth factors. This activity plays an important role in tissue remodeling and repair.

Pathways

The ADAM12 protein is key in the EGFR signaling pathway where it modulates the availability of epidermal growth factor (EGF) by shedding EGF-like ligands from the cell surface. This action connects ADAM12 with proteins such as ERBB receptors influencing cell division and growth. Another significant pathway is the TGF-beta signaling pathway where ADAM12's interaction with latent TGF-beta complexes can impact the bioavailability of this cytokine therefore relating to proliferation and differentiation of cells.

ADAM12 is linked to cancer progression and metastasis as its activity can positively influence tumor cell invasiveness and growth. Elevated levels of ADAM12 are observed in certain cancer types including breast and prostate cancer. Additionally it associates with metabolic disorders such as obesity where ADAM12 impacts adipogenesis and insulin sensitivity. In cancer ADAM12 interacts with proteins like matrix metalloproteinases (MMPs) to remodel the tumor microenvironment promoting cancer cell invasion.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).

See full target information ADAM12

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Foods (Basel, Switzerland) 14: PubMed40870677

2025

Yellow Pigments Ameliorate Hyperuricemia via Dual Mechanisms: Xanthine Oxidase Inhibition and Uric Acid Transporter Modulation (ABCG2, URAT1, and GLUT9).

Applications

Unspecified application

Species

Unspecified reactive species

Furong Xue,Renqin Zhu,Jiaxing Li,Zheng Liu,Lidan Niu,Wei Chen,Chengtao Wang,Jie Zheng

BMC medicine 20:189 PubMed35610640

2022

Role of EZH2-mediated H3K27me3 in placental ADAM12-S expression: implications for fetoplacental growth.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Nan Zhu,Xiao-Wen Gan,Fan Pan,Xiao-Tian Ni,Leslie Myatt,Wang-Sheng Wang,Kang Sun

European journal of histochemistry : EJH 65: PubMed34587717

2021

miR-29a-3p enhances the radiosensitivity of oral squamous cell carcinoma cells by inhibiting ADAM12.

Applications

Unspecified application

Species

Unspecified reactive species

Cuihong Jiang,Feng Liu,Shuai Xiao,Lili He,Wenqiong Wu,Qi Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADAM12 antibody

Western blot - Anti-ADAM12 antibody (AB223476)

Western blot - Anti-ADAM12 antibody (AB223476)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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