Goat Polyclonal ADAM12 antibody. Suitable for WB and reacts with Mouse, Rat, Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ADAM12 aa 200-250.
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Publications
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- BMC medicine 20:1892022Role of EZH2-mediated H3K27me3 in placental ADAM12-S expression: implications for fetoplacental growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYa-Nan Zhu,Xiao-Wen Gan,Fan Pan,Xiao-Tian Ni,Leslie Myatt,Wang-Sheng Wang,Kang SunPubMed 35610640
- European journal of histochemistry : EJH 65:2021miR-29a-3p enhances the radiosensitivity of oral squamous cell carcinoma cells by inhibiting ADAM12.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCuihong Jiang,Feng Liu,Shuai Xiao,Lili He,Wenqiong Wu,Qi ZhaoPubMed 34587717
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ADAM12 aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link O43184
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Cow | Predicted |
| Pig | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Rat | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Pig | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Human | Dilution info 0.10000-0.30000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
Alternative names
MLTN, UNQ346/PRO545, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, ADAM 12, Meltrin-alpha
Recommended products
Goat Polyclonal ADAM12 antibody. Suitable for WB and reacts with Mouse, Rat, Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ADAM12 aa 200-250.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ADAM12 aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link O43184
- Purification technique
- Affinity purification Immunogen
- Specificity
ab223476 is expected to recognize both reported isoforms (NP_003465.3; NP_067673.2).
- Concentration
- Purification notes
ab223476 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAM12 also known as meltrin-alpha is a member of the ADAM (a disintegrin and metalloproteinase) family and has a mass of approximately 110 kDa. It functions primarily in the modulation of cell-cell and cell-matrix interactions due to its metalloprotease and disintegrin domains. ADAM12 protein is expressed in various tissues including muscle and placenta. Although found in many cell types it is particularly abundant during embryogenesis and development reflecting its role in these processes.
- Biological function summary
The ADAM12 protein holds a role in cell signaling migration and proliferation. It is often part of complexes that interact with other extracellular matrix components influencing cell adhesion and motility. Through its protease activity ADAM12 facilitates growth factor signaling by cleaving membrane-bound precursor proteins to release active growth factors. This activity plays an important role in tissue remodeling and repair.
- Pathways
The ADAM12 protein is key in the EGFR signaling pathway where it modulates the availability of epidermal growth factor (EGF) by shedding EGF-like ligands from the cell surface. This action connects ADAM12 with proteins such as ERBB receptors influencing cell division and growth. Another significant pathway is the TGF-beta signaling pathway where ADAM12's interaction with latent TGF-beta complexes can impact the bioavailability of this cytokine therefore relating to proliferation and differentiation of cells.
- Associated diseases and disorders
ADAM12 is linked to cancer progression and metastasis as its activity can positively influence tumor cell invasiveness and growth. Elevated levels of ADAM12 are observed in certain cancer types including breast and prostate cancer. Additionally it associates with metabolic disorders such as obesity where ADAM12 impacts adipogenesis and insulin sensitivity. In cancer ADAM12 interacts with proteins like matrix metalloproteinases (MMPs) to remodel the tumor microenvironment promoting cancer cell invasion.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ADAM12 antibody (ab223476)
Primary incubation was 1 hour.
All lanes: Western blot - Anti-ADAM12 antibody (ab223476) at 0.1 µg/mL
All lanes: Human heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 100 kDa
Observed band size: 100 kDa
Western blot - Anti-ADAM12 antibody (ab223476)
Primary incubation was 1 hour.
All lanes: Western blot - Anti-ADAM12 antibody (ab223476) at 0.1 µg/mL
Lane 1: Mouse heart lysate (in RIPA buffer) at 35 µg
Lane 2: Rat heart lysate (in RIPA buffer) at 35 µg
Lane 3: Pig heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 100 kDa
Observed band size: 60 kDa, 90 kDa
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