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AB28747

Anti-ADAM12 antibody

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(4 Publications)

Goat Polyclonal ADAM12 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADAM12 aa 1-50.

View Alternative Names

MLTN, UNQ346/PRO545, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, ADAM 12, Meltrin-alpha

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ADAM12 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O43184

Specificity

This antibody is expected to recognise both the longer membrane bound form of human ADAM12 and the shorter soluble ADAM12 splice isoform. This antibody does not cross react with other ADAMS.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAM12 also known as meltrin-alpha is a member of the ADAM (a disintegrin and metalloproteinase) family and has a mass of approximately 110 kDa. It functions primarily in the modulation of cell-cell and cell-matrix interactions due to its metalloprotease and disintegrin domains. ADAM12 protein is expressed in various tissues including muscle and placenta. Although found in many cell types it is particularly abundant during embryogenesis and development reflecting its role in these processes.
Biological function summary

The ADAM12 protein holds a role in cell signaling migration and proliferation. It is often part of complexes that interact with other extracellular matrix components influencing cell adhesion and motility. Through its protease activity ADAM12 facilitates growth factor signaling by cleaving membrane-bound precursor proteins to release active growth factors. This activity plays an important role in tissue remodeling and repair.

Pathways

The ADAM12 protein is key in the EGFR signaling pathway where it modulates the availability of epidermal growth factor (EGF) by shedding EGF-like ligands from the cell surface. This action connects ADAM12 with proteins such as ERBB receptors influencing cell division and growth. Another significant pathway is the TGF-beta signaling pathway where ADAM12's interaction with latent TGF-beta complexes can impact the bioavailability of this cytokine therefore relating to proliferation and differentiation of cells.

ADAM12 is linked to cancer progression and metastasis as its activity can positively influence tumor cell invasiveness and growth. Elevated levels of ADAM12 are observed in certain cancer types including breast and prostate cancer. Additionally it associates with metabolic disorders such as obesity where ADAM12 impacts adipogenesis and insulin sensitivity. In cancer ADAM12 interacts with proteins like matrix metalloproteinases (MMPs) to remodel the tumor microenvironment promoting cancer cell invasion.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
See full target information ADAM12
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 62: PubMed36866761

2023

A disintegrin and metalloprotease 12 contributes to colorectal cancer metastasis by regulating epithelial‑mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Hyung-Hoon Oh,Young-Lan Park,Sun-Young Park,Young-Eun Joo

In vivo (Athens, Greece) 36:2194-2204 PubMed36099146

2022

Expression of in Gastric Cancer and its Relation to Tumor Cell Behavior and Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Woo Chung,Young-Lan Park,Sun-Young Park,Young-Eun Joo

Journal of experimental & clinical cancer research 34:35 PubMed25927670

2015

MicroRNA-144 inhibits the metastasis of gastric cancer by targeting MET expression.

Applications

WB

Species

Unspecified reactive species

Jun Liu,Hui Xue,Jianjun Zhang,Tao Suo,Yijin Xiang,Wen Zhang,Jun Ma,Dingfang Cai,Xixi Gu

American journal of respiratory cell and molecular 41:449-58 PubMed19213876

2009

Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium.

Applications

IHC-P

Species

Human

Cecilia Estrella,Natacha Rocks,Geneviève Paulissen,Florence Quesada-Calvo,Agnès Noël,Eva Vilain,Philippe Lassalle,Isabelle Tillie-Leblond,Didier Cataldo,Philippe Gosset
View all publications
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Product promise

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