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AB39155

Anti-ADAM12 antibody - Aminoterminal end

4

(3 Reviews)

|

(10 Publications)

Rabbit Polyclonal ADAM12 antibody. Suitable for Flow Cyt, WB, ICC/IF, IHC-Fr and reacts with Mouse, Rat, Pig, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human ADAM12.

View Alternative Names

MLTN, UNQ346/PRO545, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, ADAM 12, Meltrin-alpha

1 Images
Western blot - Anti-ADAM12 antibody - Aminoterminal end (AB39155)
  • WB

Unknown

Western blot - Anti-ADAM12 antibody - Aminoterminal end (AB39155)

All lanes:

Western blot - Anti-ADAM12 antibody - Aminoterminal end (ab39155) at 1/1000 dilution

All lanes:

Human heart tissue lysate - total protein (<a href='/en-us/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

Predicted band size: 100 kDa

Observed band size: 117 kDa,42 kDa,50 kDa,84 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Mouse, Pig

Applications

WB, IHC-Fr, Flow Cyt, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ADAM12. The exact immunogen used to generate this antibody is proprietary information.

O43184

Specificity

This antibody recognizes latent unprocessed and processed forms of ADAM12, but does not react with other ADAMs.

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody has been peptide affinity purified.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 2.9% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAM12 also known as meltrin-alpha is a member of the ADAM (a disintegrin and metalloproteinase) family and has a mass of approximately 110 kDa. It functions primarily in the modulation of cell-cell and cell-matrix interactions due to its metalloprotease and disintegrin domains. ADAM12 protein is expressed in various tissues including muscle and placenta. Although found in many cell types it is particularly abundant during embryogenesis and development reflecting its role in these processes.
Biological function summary

The ADAM12 protein holds a role in cell signaling migration and proliferation. It is often part of complexes that interact with other extracellular matrix components influencing cell adhesion and motility. Through its protease activity ADAM12 facilitates growth factor signaling by cleaving membrane-bound precursor proteins to release active growth factors. This activity plays an important role in tissue remodeling and repair.

Pathways

The ADAM12 protein is key in the EGFR signaling pathway where it modulates the availability of epidermal growth factor (EGF) by shedding EGF-like ligands from the cell surface. This action connects ADAM12 with proteins such as ERBB receptors influencing cell division and growth. Another significant pathway is the TGF-beta signaling pathway where ADAM12's interaction with latent TGF-beta complexes can impact the bioavailability of this cytokine therefore relating to proliferation and differentiation of cells.

ADAM12 is linked to cancer progression and metastasis as its activity can positively influence tumor cell invasiveness and growth. Elevated levels of ADAM12 are observed in certain cancer types including breast and prostate cancer. Additionally it associates with metabolic disorders such as obesity where ADAM12 impacts adipogenesis and insulin sensitivity. In cancer ADAM12 interacts with proteins like matrix metalloproteinases (MMPs) to remodel the tumor microenvironment promoting cancer cell invasion.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
See full target information ADAM12

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Bone research 12:27 PubMed38714649

2024

Multi-omics analysis of human tendon adhesion reveals that ACKR1-regulated macrophage migration is involved in regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xinshu Zhang,Yao Xiao,Bo Hu,Yanhao Li,Shaoyang Zhang,Jian Tian,Shuo Wang,Zaijin Tao,Xinqi Zeng,Ning-Ning Liu,Baojie Li,Shen Liu

Frontiers in oncology 11:643039 PubMed34490077

2021

The Role of LINC00284 in the Development of Thyroid Cancer Its Regulation of the MicroRNA-30d-5p-Mediated ADAM12/Notch Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmei Hu,Zhichen Kang,Lixin Guo,Fuling Qu,Rongfeng Qu

International journal of cancer 146:1281-1292 PubMed31456217

2019

Glioblastoma initiating cells are sensitive to histone demethylase inhibition due to epigenetic deregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jan-Philipp Mallm,Paul Windisch,Alva Biran,Zoltan Gal,Sabrina Schumacher,Rainer Glass,Christel Herold-Mende,Eran Meshorer,Martje Barbus,Karsten Rippe

Oncogene 37:6316-6326 PubMed30042416

2018

Adam12 and lnc015192 act as ceRNAs in breast cancer by regulating miR-34a.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojia Huang,Xinhua Xie,Peng Liu,Lu Yang,Bo Chen,Cailu Song,Hailin Tang,Xiaoming Xie

BioMed research international 2017:9645874 PubMed28357407

2017

Role of MicroRNA-103a Targeting ADAM10 in Abdominal Aortic Aneurysm.

Applications

WB

Species

Mouse

Tong Jiao,Ye Yao,Bo Zhang,Da-Cheng Hao,Qing-Feng Sun,Jing-Bo Li,Chao Yuan,Bao Jing,Yun-Peng Wang,Hai-Yang Wang

F1000Research 5:54 PubMed27335635

2016

Antibody performance in ChIP-sequencing assays: From quality scores of public data sets to quantitative certification.

Applications

Unspecified application

Species

Unspecified reactive species

Marco-Antonio Mendoza-Parra,Vincent Saravaki,Pierre-Etienne Cholley,Matthias Blum,Benjamin Billoré,Hinrich Gronemeyer

Stem cell research & therapy 6:46 PubMed25890097

2015

Sdf-1 (CXCL12) induces CD9 expression in stem cells engaged in muscle regeneration.

Applications

ICC, WB

Species

Unspecified reactive species, Unspecified reactive species

Edyta Brzoska,Kamil Kowalski,Agnieszka Markowska-Zagrajek,Magdalena Kowalewska,Rafał Archacki,Izabela Plaskota,Władysława Stremińska,Katarzyna Jańczyk-Ilach,Maria A Ciemerych

Stem cells and development 23:2455-68 PubMed24940624

2014

Competence of in vitro cultured mouse embryonic stem cells for myogenic differentiation and fusion with myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Karolina Archacka,Agnieszka Denkis,Edyta Brzóska,Barbara Świerczek,Marta Tarczyluk,Katarzyna Jańczyk-Ilach,Maria A Ciemerych,Jerzy Moraczewski

PloS one 8:e61760 PubMed23671573

2013

Adhesion proteins--an impact on skeletal myoblast differentiation.

Applications

WB, ICC/IF, IHC-Fr, IHC-Fr, ICC/IF, WB

Species

Mouse, Mouse, Mouse, Rat, Rat, Rat

Marta Przewoźniak,Iwona Czaplicka,Areta M Czerwińska,Agnieszka Markowska-Zagrajek,Jerzy Moraczewski,Władysława Stremińska,Katarzyna Jańczyk-Ilach,Maria A Ciemerych,Edyta Brzoska

American journal of respiratory cell and molecular 41:449-58 PubMed19213876

2009

Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium.

Applications

Flow Cyt

Species

Human

Cecilia Estrella,Natacha Rocks,Geneviève Paulissen,Florence Quesada-Calvo,Agnès Noël,Eva Vilain,Philippe Lassalle,Isabelle Tillie-Leblond,Didier Cataldo,Philippe Gosset
View all publications

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