Rabbit Recombinant Monoclonal ADAM15 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (AB124698), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-ab124698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109709.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (AB124698)")
![Western blot - Anti-ADAM15 antibody [EPR5619] (AB124698), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-ab124698--western-blot-img58949.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ADAM15 antibody [EPR5619] (AB124698)")
![Western blot - Anti-ADAM15 antibody [EPR5619] (AB124698), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-ab124698--western-blot-img82067.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ADAM15 antibody [EPR5619] (AB124698)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (AB124698), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-ab124698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117094.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (AB124698)")
Publications
Filter by
- Frontiers in molecular biosciences 10:11625042023Identification of membrane proteins regulated by ADAM15 by SUSPECS proteomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatteo Calligaris,Chun Y Yang,Simone Bonelli,Donatella Pia Spanò,Stephan A Müller,Stefan F Lichtenthaler,Linda Troeberg,Simone D ScilabraPubMed 37388246
- Membranes 12:2022Quantitative Proteomics Reveals That ADAM15 Can Have Proteolytic-Independent Functions in the Steady State.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Yao Yang,Simone Bonelli,Matteo Calligaris,Anna Paola Carreca,Stephan A Müller,Stefan F Lichtenthaler,Linda Troeberg,Simone D ScilabraPubMed 35736286
- Cells 10:2021A Novel Pro-Inflammatory Mechanosensing Pathway Orchestrated by the Disintegrin Metalloproteinase ADAM15 in Synovial Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomasz Janczi,Florian Meier,Yuliya Fehrl,Raimund W Kinne,Beate Böhm,Harald BurkhardtPubMed 34685689
- Journal of virology 95:2021ADAM15 Participates in Tick-Borne Encephalitis Virus Replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Yang,Rongjuan Pei,Yun Wang,Yuan Zhou,Min Yang,Xinwen Chen,Jizheng ChenPubMed 33208450
- Journal of the American Heart Association 7:e0104272018Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSayantan Jana,Hao Zhang,Gary D Lopaschuk,Darren H Freed,Consolato Sergi,Paul F Kantor,Gavin Y Oudit,Zamaneh KassiriPubMed 30371322
- Nature structural & molecular biology 25:1019-10272018A novel class of microRNA-recognition elements that function only within open reading frames.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Zhang et. al.PubMed 30297778
- Oncology reports 34:2451-602015ADAM15 targets MMP9 activity to promote lung cancer cell invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan-Dan Dong et. al.PubMed 26323669
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/800 | Notes For unpurified use 1/250.00000 - 1/500.00000. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
Alternative names
MDC15, ADAM15, Disintegrin and metalloproteinase domain-containing protein 15, ADAM 15, Metalloprotease RGD disintegrin protein, Metargidin, MDC-15
Recommended products
Rabbit Recombinant Monoclonal ADAM15 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5619
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 5.86 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAM15 also known as Metargidin is a member of the ADAM (a disintegrin and metalloproteinase) family of proteins. It has a mass of approximately 92 kDa. This protein is a transmembrane protein with metalloprotease activity which means it can cleave other transmembrane or extracellular matrix proteins. ADAM15 is expressed in various tissues including heart lung liver and kidney and is notably present in cells of the immune system and certain cancerous tissues.
- Biological function summary
ADAM15 influences cell adhesion migration and signaling pathways. It operates as part of both integrin-mediated and growth factor-mediated signaling complexes. This protein modulates interactions between integrins and the extracellular matrix impacting cellular movement and communication. By processing and regulating these interactions ADAM15 plays a role in tissue remodeling and inflammatory responses.
- Pathways
ADAM15 interacts with proteins in the integrin signaling and EGFR (epidermal growth factor receptor) pathways. This involvement makes it a critical component in cell signaling linked to proliferation differentiation and survival. Specifically ADAM15 influences integrin-related proteins such as β1-integrin and is implicated in modulating the signaling activity of EGFR. These interactions illustrate its role in the regulation of cellular dynamics.
- Associated diseases and disorders
ADAM15 has been connected to cancer and arthritis. Its overexpression has been observed in some cancer types including breast and prostate cancer where it affects tumor growth and metastasis potential. Additionally ADAM15 contributes to the pathology of arthritis by regulating inflammation and cartilage degradation. Through these processes ADAM15 works alongside proteins like matrix metalloproteinases in the disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-ab124698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109709.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling ADAM15 with purified ab124698 at 1:800 dilution (2.34 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
![Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-ab124698--western-blot-img58949.jpg "Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)")
Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)
All lanes: Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution
All lanes: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 93 kDa
Observed band size: 110 kDa, 90 kDa
![Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-ab124698--western-blot-img82067.jpg "Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)")
Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698)
ab124698 also appears to recognize the pro-protein (110kDa). This image was produced using unpurified antibody.
All lanes: Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution
All lanes: SW480 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP
Predicted band size: 93 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-ab124698--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117094.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (ab124698)
ab124698, at 1/250 dilution, staining ADAM15 in paraffin-embedded Human colon tissue by immunohistochemistry. This image was produced using unpurified antibody.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com