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AB124698

Anti-ADAM15 antibody [EPR5619]

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(9 Publications)

Rabbit Recombinant Monoclonal ADAM15 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 9 publications.

View Alternative Names

MDC15, ADAM15, Disintegrin and metalloproteinase domain-containing protein 15, ADAM 15, Metalloprotease RGD disintegrin protein, Metargidin, MDC-15

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (AB124698)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (AB124698)

ab124698, at 1/250 dilution, staining ADAM15 in paraffin-embedded Human colon tissue by immunohistochemistry. This image was produced using unpurified antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (AB124698)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] (AB124698)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling ADAM15 with purified ab124698 at 1 : 800 dilution (2.34 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

Western blot - Anti-ADAM15 antibody [EPR5619] (AB124698)
  • WB

Unknown

Western blot - Anti-ADAM15 antibody [EPR5619] (AB124698)

ab124698 also appears to recognize the pro-protein (110kDa). This image was produced using unpurified antibody.

All lanes:

Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution

All lanes:

SW480 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP

Predicted band size: 93 kDa

false

Western blot - Anti-ADAM15 antibody [EPR5619] (AB124698)
  • WB

Unknown

Western blot - Anti-ADAM15 antibody [EPR5619] (AB124698)

All lanes:

Western blot - Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution

All lanes:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 93 kDa

Observed band size: 110 kDa,90 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5619

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": "<p><strong>For unpurified use 1/250 - 1/500.</strong></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAM15 also known as Metargidin is a member of the ADAM (a disintegrin and metalloproteinase) family of proteins. It has a mass of approximately 92 kDa. This protein is a transmembrane protein with metalloprotease activity which means it can cleave other transmembrane or extracellular matrix proteins. ADAM15 is expressed in various tissues including heart lung liver and kidney and is notably present in cells of the immune system and certain cancerous tissues.
Biological function summary

ADAM15 influences cell adhesion migration and signaling pathways. It operates as part of both integrin-mediated and growth factor-mediated signaling complexes. This protein modulates interactions between integrins and the extracellular matrix impacting cellular movement and communication. By processing and regulating these interactions ADAM15 plays a role in tissue remodeling and inflammatory responses.

Pathways

ADAM15 interacts with proteins in the integrin signaling and EGFR (epidermal growth factor receptor) pathways. This involvement makes it a critical component in cell signaling linked to proliferation differentiation and survival. Specifically ADAM15 influences integrin-related proteins such as β1-integrin and is implicated in modulating the signaling activity of EGFR. These interactions illustrate its role in the regulation of cellular dynamics.

ADAM15 has been connected to cancer and arthritis. Its overexpression has been observed in some cancer types including breast and prostate cancer where it affects tumor growth and metastasis potential. Additionally ADAM15 contributes to the pathology of arthritis by regulating inflammation and cartilage degradation. Through these processes ADAM15 works alongside proteins like matrix metalloproteinases in the disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
See full target information ADAM15
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 22:34 PubMed40260066

2025

A disintegrin-like and metalloproteinase 15 facilitates glioblastoma proliferation and metastasis through activation of the protease-activated receptor 1.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Ren,Zuowei Li,Qiong Fang

PloS one 19:e0298802 PubMed38394161

2024

Digital spatial profiling of segmental outflow regions in trabecular meshwork reveals a role for ADAM15.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer A Faralli,Mark S Filla,Yong-Feng Yang,Ying Ying Sun,Kassidy Johns,Kate E Keller,Donna M Peters

Frontiers in molecular biosciences 10:1162504 PubMed37388246

2023

Identification of membrane proteins regulated by ADAM15 by SUSPECS proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Calligaris,Chun Y Yang,Simone Bonelli,Donatella Pia Spanò,Stephan A Müller,Stefan F Lichtenthaler,Linda Troeberg,Simone D Scilabra

Membranes 12: PubMed35736286

2022

Quantitative Proteomics Reveals That ADAM15 Can Have Proteolytic-Independent Functions in the Steady State.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yao Yang,Simone Bonelli,Matteo Calligaris,Anna Paola Carreca,Stephan A Müller,Stefan F Lichtenthaler,Linda Troeberg,Simone D Scilabra

Cells 10: PubMed34685689

2021

A Novel Pro-Inflammatory Mechanosensing Pathway Orchestrated by the Disintegrin Metalloproteinase ADAM15 in Synovial Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Tomasz Janczi,Florian Meier,Yuliya Fehrl,Raimund W Kinne,Beate Böhm,Harald Burkhardt

Journal of virology 95: PubMed33208450

2021

ADAM15 Participates in Tick-Borne Encephalitis Virus Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Yang,Rongjuan Pei,Yun Wang,Yuan Zhou,Min Yang,Xinwen Chen,Jizheng Chen

Journal of the American Heart Association 7:e010427 PubMed30371322

2018

Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Sayantan Jana,Hao Zhang,Gary D Lopaschuk,Darren H Freed,Consolato Sergi,Paul F Kantor,Gavin Y Oudit,Zamaneh Kassiri

Nature structural & molecular biology 25:1019-1027 PubMed30297778

2018

A novel class of microRNA-recognition elements that function only within open reading frames.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Xiaorong Zhang,Zhiqiang Cai,Jie Zhou,Ran Cao,Ya Zhao,Zonggui Chen,Dehe Wang,Wen Ruan,Qian Zhao,Guangqiao Liu,Yuanchao Xue,Yan Qin,Bing Zhou,Ligang Wu,Timothy Nilsen,Yu Zhou,Xiang-Dong Fu

Oncology reports 34:2451-60 PubMed26323669

2015

ADAM15 targets MMP9 activity to promote lung cancer cell invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Dong,Hui Zhou,Gao Li
View all publications
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Product promise

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