Rabbit Recombinant Monoclonal ADAM15 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] - BSA and Azide free (AB232389), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-bsa-and-azide-free-ab232389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109709.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] - BSA and Azide free (AB232389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] - BSA and Azide free (AB232389), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-bsa-and-azide-free-ab232389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117094.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] - BSA and Azide free (AB232389)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
Alternative names
MDC15, ADAM15, Disintegrin and metalloproteinase domain-containing protein 15, ADAM 15, Metalloprotease RGD disintegrin protein, Metargidin, MDC-15
Recommended products
Rabbit Recombinant Monoclonal ADAM15 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5619
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232389 is the carrier-free version of Anti-ADAM15 antibody [EPR5619] ab124698.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAM15 also known as Metargidin is a member of the ADAM (a disintegrin and metalloproteinase) family of proteins. It has a mass of approximately 92 kDa. This protein is a transmembrane protein with metalloprotease activity which means it can cleave other transmembrane or extracellular matrix proteins. ADAM15 is expressed in various tissues including heart lung liver and kidney and is notably present in cells of the immune system and certain cancerous tissues.
- Biological function summary
ADAM15 influences cell adhesion migration and signaling pathways. It operates as part of both integrin-mediated and growth factor-mediated signaling complexes. This protein modulates interactions between integrins and the extracellular matrix impacting cellular movement and communication. By processing and regulating these interactions ADAM15 plays a role in tissue remodeling and inflammatory responses.
- Pathways
ADAM15 interacts with proteins in the integrin signaling and EGFR (epidermal growth factor receptor) pathways. This involvement makes it a critical component in cell signaling linked to proliferation differentiation and survival. Specifically ADAM15 influences integrin-related proteins such as β1-integrin and is implicated in modulating the signaling activity of EGFR. These interactions illustrate its role in the regulation of cellular dynamics.
- Associated diseases and disorders
ADAM15 has been connected to cancer and arthritis. Its overexpression has been observed in some cancer types including breast and prostate cancer where it affects tumor growth and metastasis potential. Additionally ADAM15 contributes to the pathology of arthritis by regulating inflammation and cartilage degradation. Through these processes ADAM15 works alongside proteins like matrix metalloproteinases in the disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] - BSA and Azide free (ab232389), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-bsa-and-azide-free-ab232389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109709.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] - BSA and Azide free (ab232389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] - BSA and Azide free (ab232389)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling ADAM15 with purified Anti-ADAM15 antibody [EPR5619] ab124698 at 1:800 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ADAM15 antibody [EPR5619] ab124698).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] - BSA and Azide free (ab232389), expandable thumbnail](https://content.abcam.com/products/images/adam15-antibody-epr5619-bsa-and-azide-free-ab232389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117094.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] - BSA and Azide free (ab232389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody [EPR5619] - BSA and Azide free (ab232389)
Anti-ADAM15 antibody [EPR5619] ab124698, at 1/250 dilution, staining ADAM15 in paraffin-embedded Human colon tissue by immunohistochemistry. This image was produced using unpurified antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ADAM15 antibody [EPR5619] ab124698).
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