Rabbit Polyclonal ADAM15 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADAM15 aa 1-300.
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Publications
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- Journal of virology 95:2021ADAM15 Participates in Tick-Borne Encephalitis Virus Replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Yang,Rongjuan Pei,Yun Wang,Yuan Zhou,Min Yang,Xinwen Chen,Jizheng ChenPubMed 33208450
- PloS one 9:e1102862014MicroRNA-147b regulates vascular endothelial barrier function by targeting ADAM15 expression.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ADAM15 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13444
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes or Tris-EDTA buffer (pH8.0) Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Associated Products
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Target data
Function
Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.
Alternative names
MDC15, ADAM15, Disintegrin and metalloproteinase domain-containing protein 15, ADAM 15, Metalloprotease RGD disintegrin protein, Metargidin, MDC-15
Recommended products
Rabbit Polyclonal ADAM15 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADAM15 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ADAM15 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13444
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAM15 also known as Metargidin is a member of the ADAM (a disintegrin and metalloproteinase) family of proteins. It has a mass of approximately 92 kDa. This protein is a transmembrane protein with metalloprotease activity which means it can cleave other transmembrane or extracellular matrix proteins. ADAM15 is expressed in various tissues including heart lung liver and kidney and is notably present in cells of the immune system and certain cancerous tissues.
- Biological function summary
ADAM15 influences cell adhesion migration and signaling pathways. It operates as part of both integrin-mediated and growth factor-mediated signaling complexes. This protein modulates interactions between integrins and the extracellular matrix impacting cellular movement and communication. By processing and regulating these interactions ADAM15 plays a role in tissue remodeling and inflammatory responses.
- Pathways
ADAM15 interacts with proteins in the integrin signaling and EGFR (epidermal growth factor receptor) pathways. This involvement makes it a critical component in cell signaling linked to proliferation differentiation and survival. Specifically ADAM15 influences integrin-related proteins such as β1-integrin and is implicated in modulating the signaling activity of EGFR. These interactions illustrate its role in the regulation of cellular dynamics.
- Associated diseases and disorders
ADAM15 has been connected to cancer and arthritis. Its overexpression has been observed in some cancer types including breast and prostate cancer where it affects tumor growth and metastasis potential. Additionally ADAM15 contributes to the pathology of arthritis by regulating inflammation and cartilage degradation. Through these processes ADAM15 works alongside proteins like matrix metalloproteinases in the disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-ADAM15 antibody - N-terminal (ab137387)
7.5% SDS PAGE
All lanes: Western blot - Anti-ADAM15 antibody - N-terminal (ab137387) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 85 kDa, 92 kDa, 93 kDa, 97 kDa
Western blot - Anti-ADAM15 antibody - N-terminal (ab137387)
7.5% SDS PAGE
All lanes: Western blot - Anti-ADAM15 antibody - N-terminal (ab137387) at 1/1000 dilution
All lanes: NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 103 kDa, 93 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM15 antibody - N-terminal (ab137387)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle, labelling ADAM15 with ab137387 at 10µg/ml.
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