Anti-ADAM17 antibody (ab39162) is a rabbit polyclonal antibody that is used to detect ADAM17 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Pig, Rat samples.
- Over 40 publications
- Trusted since 2006
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- Medicina (Kaunas, Lithuania) 59:2023Investigation of CD56, ADAM17 and FGF21 Expressions in the Placentas of Preeclampsia Cases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrem Darka Aslan,Gorker Sel,Figen Barut,Rabia Baser Acikgoz,Sibel Balci,Ulku Ozmen,Aykut Barut,Muge Harma,Mehmet Ibrahim HarmaPubMed 37374349
- Cellular and molecular life sciences : CMLS 80:1352023A structural model of the iRhom-ADAM17 sheddase complex reveals functional insights into its trafficking and activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSelcan Kahveci-Türköz,Katharina Bläsius,Justyna Wozniak,Cindy Rinkens,Anke Seifert,Petr Kasparek,Henrike Ohm,Shixin Oltzen,Martin Nieszporek,Nicole Schwarz,Aaron Babendreyer,Christian Preisinger,Radislav Sedlacek,Andreas Ludwig,Stefan DüsterhöftPubMed 37119365
- International journal of molecular sciences 24:2023ADAM17 Confers Temozolomide Resistance in Human Glioblastoma Cells and miR-145 Regulates Its Expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJen-Tsung Yang,I-Neng Lee,Cheng Huang,Hsiu-Chen Huang,Yu-Ping Wu,Zhi-Yong Chong,Jui-Chieh ChenPubMed 37175410
- Life science alliance 6:2023The ADAM17 sheddase complex regulator iTAP/Frmd8 modulates inflammation and tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina Badenes,Emma Burbridge,Ioanna Oikonomidi,Abdulbasit Amin,Érika de Carvalho,Lindsay Kosack,Camila Mariano,Pedro Domingos,Pedro Faísca,Colin AdrainPubMed 36720499
- Frontiers in cell and developmental biology 11:10215952023Cardiomyocyte cohesion is increased after ADAM17 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Shoykhet,Jens Waschke,Sunil YeruvaPubMed 36733457
- Journal of orthopaedic translation 36:132-1442022Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Ji,Yueyang Lu,Zhuoyi Ma,Ke Gan,Yan Liu,Yue Cheng,Junliang Xu,Shijia Liu,Yunke Guo,Shanhang Han,Zengyan Zhao,Hanmei Xu,Weiyan QiPubMed 36185580
- American journal of physiology. Heart and circulatory physiology 323:H688-H7012022ADAM17 cleaves the insulin receptor ectodomain on endothelial cells and causes vascular insulin resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThaysa Ghiarone,Jorge A Castorena-Gonzalez,Christopher A Foote,Francisco I Ramirez-Perez,Larissa Ferreira-Santos,Francisco J Cabral-Amador,Roger de la Torre,Rama R Ganga,Andrew A Wheeler,Camila Manrique-Acevedo,Jaume Padilla,Luis A Martinez-LemusPubMed 36018759
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e222342022The collectrin-like part of the SARS-CoV-1 and -2 receptor ACE2 is shed by the metalloproteinases ADAM10 and ADAM17.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRabea Victoria Niehues,Justyna Wozniak,Florian Wiersch,Eva Lilienthal,Nikola Tacken,Tim Schumertl,Christoph Garbers,Andreas Ludwig,Stefan DüsterhöftPubMed 35199397
- Viruses 14:2022ADAM17 Is an Essential Factor for the Infection of Bovine Cells with Pestiviruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarianne Zaruba,Hann-Wei Chen,Ole Frithjof Pietsch,Kati Szakmary-Braendle,Angelika Auer,Marlene Mötz,Kerstin Seitz,Stefan Düsterhöft,Aspen M Workman,Till Rümenapf,Christiane RiedelPubMed 35215974
- Journal of neuroinflammation 19:462022The role of S100B/RAGE-enhanced ADAM17 activation in endothelial glycocalyx shedding after traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhimin Zou,Li Li,Qin Li,Peng Zhao,Kun Zhang,Chengyong Liu,Daozhang Cai,Marc Maegele,Zhengtao Gu,Qiaobing HuangPubMed 35148784
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ADAM17. The exact immunogen used to generate this antibody is proprietary information. Database link P78536
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected |
| Rat | Predicted | Expected | Expected | Expected |
| Pig | Predicted | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
Species Pig | Dilution info - | Notes 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
Species Human | Dilution info - | Notes 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins including adhesion proteins, growth factor precursors and cytokines important for inflammation and immunity (PubMed:24226769, PubMed:24227843, PubMed:28060820, PubMed:28923481). Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form (PubMed:36078095, PubMed:9034191). Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) (PubMed:24226769). Plays a role in the proteolytic processing of ACE2 (PubMed:24227843). Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (By similarity). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity). Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R (PubMed:26876177, PubMed:28060820). Mediates the proteolytic cleavage and shedding of FCGR3A upon NK cell stimulation, a mechanism that allows for increased NK cell motility and detachment from opsonized target cells. Cleaves TREM2, resulting in shedding of the TREM2 ectodomain (PubMed:28923481).
Alternative names
CD156b, CSVP, TACE, ADAM17, Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme
Recommended products
Anti-ADAM17 antibody (ab39162) is a rabbit polyclonal antibody that is used to detect ADAM17 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Pig, Rat samples.
- Over 40 publications
- Trusted since 2006
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ADAM17. The exact immunogen used to generate this antibody is proprietary information. Database link P78536
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody recognizes ADAM17, but does not react with other ADAMs.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Anti-ADAM17 antibody (ab39162) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Pig, Rat samples.
What is the molecular weight of ADAM17?
Anti-ADAM17 (ab39162) specifically detects a band for ADAM17 (UniProt: P78536) at a molecular weight of 93kDa.
Trusted by the scientific community
Anti-ADAM17 (ab39162) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-ADAM17 (ab39162) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAM17 also known as TACE (tumor necrosis factor-alpha converting enzyme) is a metalloprotease belonging to the ADAM (a disintegrin and metalloprotease) family. This protein functions by cleaving and shedding extracellular portions of cell surface proteins. It weighs approximately 93 kDa. ADAM17 is expressed in a range of tissues including the heart liver kidney and brain. The protein plays a role in the regulation of various biological processes through the activation and inactivation of membrane-bound precursor proteins.
- Biological function summary
The ADAM17 protein regulates a multitude of cellular responses such as inflammation and growth factor signaling. It is not part of a complex but interacts with various substrates in the cell membrane. ADAM17 cleaves precursors to release soluble cytokines and growth factors like tumor necrosis factor-alpha (TNF-alpha) and epidermal growth factor receptor (EGFR) ligands which modulate cell proliferation migration and apoptosis.
- Pathways
ADAM17 plays an integral role in both the TNF and EGFR signaling pathways. These pathways are important in maintaining normal cellular homeostasis. ADAM17 interacts with proteins like TNF receptors and ligands of the EGFR family integrating signaling that affects immune response and cell growth. The regulatory function of ADAM17 in these pathways makes it an important target for modulating cellular responses triggered by external stimuli.
- Associated diseases and disorders
ADAM17 has significant implications in conditions such as rheumatoid arthritis and cancer. In rheumatoid arthritis the protein's shedding activity influences inflammatory cytokines contributing to the disease’s pathogenesis alongside proteins like TNF-alpha. In cancer ADAM17's ability to release EGFR ligands affects tumor cell proliferation and metastasis with interactions involving EGFR proteins. It is a target of therapeutic interest with research focused on developing inhibitors to manage these diseases effectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM17 antibody (ab39162)
ab39162 staining human normal colon. Staining is localised to membrane.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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