Rabbit Polyclonal ADAM17 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Rat, Pig samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human ADAM17.
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Publications
- Medicina (Kaunas, Lithuania) 59:2023Investigation of CD56, ADAM17 and FGF21 Expressions in the Placentas of Preeclampsia Cases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrem Darka Aslan,Gorker Sel,Figen Barut,Rabia Baser Acikgoz,Sibel Balci,Ulku Ozmen,Aykut Barut,Muge Harma,Mehmet Ibrahim HarmaPubMed 37374349
- Cellular and molecular life sciences : CMLS 80:1352023A structural model of the iRhom-ADAM17 sheddase complex reveals functional insights into its trafficking and activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSelcan Kahveci-Türköz,Katharina Bläsius,Justyna Wozniak,Cindy Rinkens,Anke Seifert,Petr Kasparek,Henrike Ohm,Shixin Oltzen,Martin Nieszporek,Nicole Schwarz,Aaron Babendreyer,Christian Preisinger,Radislav Sedlacek,Andreas Ludwig,Stefan DüsterhöftPubMed 37119365
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human ADAM17. The exact immunogen used to generate this antibody is proprietary information. Database link P78536
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected |
| Rat | Predicted | Expected | Expected | Expected |
| Pig | Predicted | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
Species Pig | Dilution info - | Notes 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
Species Human | Dilution info - | Notes 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Pig, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form (PubMed:9034191). Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) (PubMed:24226769). Plays a role in the proteolytic processing of ACE2 (PubMed:24227843). Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (By similarity). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity). Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R (PubMed:26876177, PubMed:28060820).
Alternative names
Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, ADAM17, TACE, CSVP
Recommended products
Rabbit Polyclonal ADAM17 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Rat, Pig samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human ADAM17.
Alternative names
Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, ADAM17, TACE, CSVP
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human ADAM17. The exact immunogen used to generate this antibody is proprietary information. Database link P78536
- Purification technique
Affinity purification Immunogen
- Specificity
This antibody recognizes ADAM17, but does not react with other ADAMs.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
ADAM17 also known as TACE (tumor necrosis factor-alpha converting enzyme) is a metalloprotease belonging to the ADAM (a disintegrin and metalloprotease) family. This protein functions by cleaving and shedding extracellular portions of cell surface proteins. It weighs approximately 93 kDa. ADAM17 is expressed in a range of tissues including the heart liver kidney and brain. The protein plays a role in the regulation of various biological processes through the activation and inactivation of membrane-bound precursor proteins.
- Biological function summary
The ADAM17 protein regulates a multitude of cellular responses such as inflammation and growth factor signaling. It is not part of a complex but interacts with various substrates in the cell membrane. ADAM17 cleaves precursors to release soluble cytokines and growth factors like tumor necrosis factor-alpha (TNF-alpha) and epidermal growth factor receptor (EGFR) ligands which modulate cell proliferation migration and apoptosis.
- Pathways
ADAM17 plays an integral role in both the TNF and EGFR signaling pathways. These pathways are important in maintaining normal cellular homeostasis. ADAM17 interacts with proteins like TNF receptors and ligands of the EGFR family integrating signaling that affects immune response and cell growth. The regulatory function of ADAM17 in these pathways makes it an important target for modulating cellular responses triggered by external stimuli.
- Associated diseases and disorders
ADAM17 has significant implications in conditions such as rheumatoid arthritis and cancer. In rheumatoid arthritis the protein's shedding activity influences inflammatory cytokines contributing to the disease’s pathogenesis alongside proteins like TNF-alpha. In cancer ADAM17's ability to release EGFR ligands affects tumor cell proliferation and metastasis with interactions involving EGFR proteins. It is a target of therapeutic interest with research focused on developing inhibitors to manage these diseases effectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM17 antibody (ab39162)
ab39162 staining human normal colon. Staining is localised to membrane.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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