Rabbit Monoclonal ADAM17 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (AB291100), expandable thumbnail](https://content.abcam.com/products/images/adam17-antibody-epr21129-131-bsa-and-azide-free-ab291100--western-blot-img400861.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (AB291100)")
![Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (AB291100), expandable thumbnail](https://content.abcam.com/products/images/adam17-antibody-epr21129-131-bsa-and-azide-free-ab291100--western-blot-img400860.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (AB291100)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins including adhesion proteins, growth factor precursors and cytokines important for inflammation and immunity (PubMed:24226769, PubMed:24227843, PubMed:28060820, PubMed:28923481). Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form (PubMed:36078095, PubMed:9034191). Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT) (PubMed:24226769). Plays a role in the proteolytic processing of ACE2 (PubMed:24227843). Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (By similarity). Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (By similarity). Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R (PubMed:26876177, PubMed:28060820). Mediates the proteolytic cleavage and shedding of FCGR3A upon NK cell stimulation, a mechanism that allows for increased NK cell motility and detachment from opsonized target cells. Cleaves TREM2, resulting in shedding of the TREM2 ectodomain (PubMed:28923481).
Alternative names
CD156b, CSVP, TACE, ADAM17, Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme
Recommended products
Rabbit Monoclonal ADAM17 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21129-131
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAM17 also known as TACE (tumor necrosis factor-alpha converting enzyme) is a metalloprotease belonging to the ADAM (a disintegrin and metalloprotease) family. This protein functions by cleaving and shedding extracellular portions of cell surface proteins. It weighs approximately 93 kDa. ADAM17 is expressed in a range of tissues including the heart liver kidney and brain. The protein plays a role in the regulation of various biological processes through the activation and inactivation of membrane-bound precursor proteins.
- Biological function summary
The ADAM17 protein regulates a multitude of cellular responses such as inflammation and growth factor signaling. It is not part of a complex but interacts with various substrates in the cell membrane. ADAM17 cleaves precursors to release soluble cytokines and growth factors like tumor necrosis factor-alpha (TNF-alpha) and epidermal growth factor receptor (EGFR) ligands which modulate cell proliferation migration and apoptosis.
- Pathways
ADAM17 plays an integral role in both the TNF and EGFR signaling pathways. These pathways are important in maintaining normal cellular homeostasis. ADAM17 interacts with proteins like TNF receptors and ligands of the EGFR family integrating signaling that affects immune response and cell growth. The regulatory function of ADAM17 in these pathways makes it an important target for modulating cellular responses triggered by external stimuli.
- Associated diseases and disorders
ADAM17 has significant implications in conditions such as rheumatoid arthritis and cancer. In rheumatoid arthritis the protein's shedding activity influences inflammatory cytokines contributing to the disease’s pathogenesis alongside proteins like TNF-alpha. In cancer ADAM17's ability to release EGFR ligands affects tumor cell proliferation and metastasis with interactions involving EGFR proteins. It is a target of therapeutic interest with research focused on developing inhibitors to manage these diseases effectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100), expandable thumbnail](https://content.abcam.com/products/images/adam17-antibody-epr21129-131-bsa-and-azide-free-ab291100--western-blot-img400861.jpg "Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)")
Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)
This data was developed using Anti-ADAM17 antibody [EPR21129-131] ab291073, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Lysates were prepared from fresh material and used for Western blotting immediately to minimize protein degradation.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-ADAM17 antibody [EPR21129-131] (Anti-ADAM17 antibody [EPR21129-131] ab291073) at 1/1000 dilution
Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell fresh lysate
Lane 2: Jurkat (human T cell leukemia T lymphocyte) whole cell fresh lysate
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 135 kDa, 80 kDa
Exposure time: 3min
![Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100), expandable thumbnail](https://content.abcam.com/products/images/adam17-antibody-epr21129-131-bsa-and-azide-free-ab291100--western-blot-img400860.jpg "Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)")
Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (ab291100)
This data was developed using Anti-ADAM17 antibody [EPR21129-131] ab291073, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST
80 kDa mature ADAM17 and 135 kDa proform ADAM17 are observed.
The expression profile is consistent with that described in the literature (PMID: 27731361).
In Western blot, Anti-ADAM17 antibody [EPR21129-131] ab291073 was shown to bind specifically to ADAM17. Two bands were observed at 135/80 kDa in wild-type HCT116 cell lysates with no signal observed at this size in ADAM17 knockout cell lysates.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Performed under reducing conditions.
All lanes: Western blot - Anti-ADAM17 antibody [EPR21129-131] (Anti-ADAM17 antibody [EPR21129-131] ab291073) at 1/1000 dilution
Lane 1: Wild-type HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate 20 µg
Lane 2: ADAM17 knockout HCT116 whole cell lysate 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 µg
Lane 4: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 135 kDa, 80 kDa
Exposure time: 3min
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