Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (AB291100)

This data was developed using ab291073, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

Lysates were prepared from fresh material and used for Western blotting immediately to minimize protein degradation.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-ADAM17 antibody [EPR21129-131] (<a href='/en-us/products/primary-antibodies/adam17-antibody-epr21129-131-ab291073'>ab291073</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell fresh lysate

Lane 2:

Jurkat (human T cell leukemia T lymphocyte) whole cell fresh lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 135 kDa,80 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (AB291100)

This data was developed using ab291073, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

80 kDa mature ADAM17 and 135 kDa proform ADAM17 are observed.

The expression profile is consistent with that described in the literature (PMID : 27731361).

In Western blot, ab291073 was shown to bind specifically to ADAM17. Two bands were observed at 135/80 kDa in wild-type HCT116 cell lysates with no signal observed at this size in ADAM17 knockout cell lysates.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Performed under reducing conditions.

All lanes:

Western blot - Anti-ADAM17 antibody [EPR21129-131] (<a href='/en-us/products/primary-antibodies/adam17-antibody-epr21129-131-ab291073'>ab291073</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT116 (human colorectal carcinoma epithelial cell) whole cell lysate 20 µg

Lane 2:

ADAM17 knockout HCT116 whole cell lysate 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 µg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 135 kDa,80 kDa

true

Exposure time: 3min

• WB

Lab

Western blot - Anti-ADAM17 antibody [EPR21129-131] - BSA and Azide free (AB291100)

This data was developed using ab291073, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

The expression profile is consistent with that described in the literature (PMID : 36720499).,

All lanes:

Western blot - Anti-ADAM17 antibody [EPR21129-131] (<a href='/en-us/products/primary-antibodies/adam17-antibody-epr21129-131-ab291073'>ab291073</a>) at 1/1000 dilution

Lane 1:

HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Lane 5:

Human ovary tissue lysate at 20 µg

Lane 6:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80-135 kDa

false

Exposure time: 40s