Anti-ADAM22 antibody [EPR28037-56]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] (AB314442)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling ADAM22 with ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on human lung.The section was incubated with ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] (AB314442)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ADAM22 with ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum.The section was incubated with ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-ADAM22 antibody [EPR28037-56] (AB314442)

ADAM22 was immunoprecipitated from 0.35 mg SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate with ab314442 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314442 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate Lane 2 : ab314442 IP in SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314442 in SK-N-SH whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 84s.

All lanes:

Immunoprecipitation - Anti-ADAM22 antibody [EPR28037-56] (ab314442) at 1/30 dilution

All lanes:

SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 84s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] (AB314442)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ADAM22 with ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum.The section was incubated with ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] (AB314442)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling ADAM22 with ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse lung.The section was incubated with ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] (AB314442)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling ADAM22 with ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on rat lung.The section was incubated with ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] (AB314442)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ADAM22 with ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum.The section was incubated with ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-ADAM22 antibody [EPR28037-56] (AB314442)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : lung, testis The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 22608556). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ADAM22 antibody [EPR28037-56] (ab314442) at 1/1000 dilution

Lane 1:

Human hippocampus tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Lane 3:

Human lung tissue lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75-90 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-ADAM22 antibody [EPR28037-56] (AB314442)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver, lung, spleen The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 22608556). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-ADAM22 antibody [EPR28037-56] (ab314442) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75-90 kDa

false

Exposure time: 180s