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Rabbit Recombinant Monoclonal ADAM22 antibody. Carrier free. Suitable for WB, IP, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (AB314443), expandable thumbnail
  • Immunoprecipitation - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (AB314443), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (AB314443), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (AB314443), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (AB314443), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Expected
Tested
Not recommended
Not recommended
Rat
Tested
Expected
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein (PubMed:19692335). Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.

Alternative names

MDC2, ADAM22, Disintegrin and metalloproteinase domain-containing protein 22, ADAM 22, Metalloproteinase-disintegrin ADAM22-3

Recommended products

Rabbit Recombinant Monoclonal ADAM22 antibody. Carrier free. Suitable for WB, IP, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28037-56
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ADAM22 also known as ADAM 22 or ADAM 2 is a protein that functions mechanically as a transmembrane receptor involved in cell-cell and cell-matrix interactions. This protein does not exhibit protease activity unlike other members of the ADAM family which distinguishes its functional role. ADAM22 has a mass of approximately 100 kDa. It is expressed predominantly in the central nervous system particularly in brain tissues where it plays a significant role in neuronal signaling.

Biological function summary

ADAM22 affects the nervous system's development and function by mediating synaptic formation and maintenance. It belongs to a protein complex with LGI1 which also interacts with other proteins on the synaptic membrane facilitating communication between neurons. The LGI1-ADAM22 complex reportedly regulates ion channels and other synaptic proteins impacting neurotransmission and synaptic plasticity essential for cognitive processes.

Pathways

ADAM22 acts within the neuropharmacological and synaptogenesis pathways. It interacts closely with LGI1 and PSD95 pivotal proteins in developing synaptic signaling platforms. These pathways influence neurotransmitter release and receptor clustering fundamental for effective neural circuit function. The disruption of this pathway or its elements can lead to neurological impairments and highlight ADAM22's role in maintaining neural integrity.

Associated diseases and disorders

ADAM22 connects to neurological conditions such as epilepsy and glioma. Mutations or deficiencies in the LGI1-ADAM22 interaction have been linked to epilepsy affecting ion channel regulatory actions. In glioma altered expression of ADAM22 may play a role in tumor progression influenced by interactions with integrins and growth factors. Therefore ADAM22's presence and regulatory actions contribute to neurological and oncological disease models.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443), expandable thumbnail

    Western blot - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443)

    This data was developed using Anti-ADAM22 antibody [EPR28037-56] ab314442, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: lung, testis
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22608556).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ADAM22 antibody [EPR28037-56] (Anti-ADAM22 antibody [EPR28037-56] ab314442) at 1/1000 dilution

    Lane 1: Human hippocampus tissue lysate at 20 µg

    Lane 2: Human cerebellum tissue lysate at 20 µg

    Lane 3: Human lung tissue lysate at 20 µg

    Lane 4: Human testis tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 75-90 kDa

    Exposure time: 10s

  • Immunoprecipitation - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443), expandable thumbnail

    Immunoprecipitation - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443)

    This data was developed using Anti-ADAM22 antibody [EPR28037-56] ab314442, the same antibody clone in a different buffer formulation.
    ADAM22 was immunoprecipitated from 0.35 mg SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate with Anti-ADAM22 antibody [EPR28037-56] ab314442 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ADAM22 antibody [EPR28037-56] ab314442 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate
    Lane 2: Anti-ADAM22 antibody [EPR28037-56] ab314442 IP in SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ADAM22 antibody [EPR28037-56] ab314442 in SK-N-SH whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-ADAM22 antibody [EPR28037-56] (Anti-ADAM22 antibody [EPR28037-56] ab314442) at 1/30 dilution

    All lanes: SK-N-SH (human neuroblastoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 84s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443)

    This data was developed using Anti-ADAM22 antibody [EPR28037-56] ab314442, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ADAM22 with Anti-ADAM22 antibody [EPR28037-56] ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum.The section was incubated with Anti-ADAM22 antibody [EPR28037-56] ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443)

    This data was developed using Anti-ADAM22 antibody [EPR28037-56] ab314442, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling ADAM22 with Anti-ADAM22 antibody [EPR28037-56] ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse lung.The section was incubated with Anti-ADAM22 antibody [EPR28037-56] ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443)

    This data was developed using Anti-ADAM22 antibody [EPR28037-56] ab314442, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling ADAM22 with Anti-ADAM22 antibody [EPR28037-56] ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human lung.The section was incubated with Anti-ADAM22 antibody [EPR28037-56] ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443), expandable thumbnail

    Western blot - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443)

    This data was developed using Anti-ADAM22 antibody [EPR28037-56] ab314442, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: liver, lung, spleen
    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22608556).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ADAM22 antibody [EPR28037-56] (Anti-ADAM22 antibody [EPR28037-56] ab314442) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse lung tissue lysate at 20 µg

    Lane 4: Mouse spleen tissue lysate at 20 µg

    Lane 5: Rat cerebellum tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 75-90 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443)

    This data was developed using Anti-ADAM22 antibody [EPR28037-56] ab314442, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling ADAM22 with Anti-ADAM22 antibody [EPR28037-56] ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat lung.The section was incubated with Anti-ADAM22 antibody [EPR28037-56] ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443)

    This data was developed using Anti-ADAM22 antibody [EPR28037-56] ab314442, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ADAM22 with Anti-ADAM22 antibody [EPR28037-56] ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum.The section was incubated with Anti-ADAM22 antibody [EPR28037-56] ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM22 antibody [EPR28037-56] - BSA and Azide free (ab314443)

    This data was developed using Anti-ADAM22 antibody [EPR28037-56] ab314442, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ADAM22 with Anti-ADAM22 antibody [EPR28037-56] ab314442 at 1/1000 (0.504 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum.The section was incubated with Anti-ADAM22 antibody [EPR28037-56] ab314442 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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