Rabbit Polyclonal ADAM23 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ADAM23.
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Publications
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- Molecular cancer 18:992019miR-301a promotes lung tumorigenesis by suppressing Runx3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXun Li et. al.PubMed 31122259
- Nature communications 9:15462018Structural basis of epilepsy-related ligand-receptor complex LGI1-ADAM22.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAtsushi Yamagata et. al.PubMed 29670100
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 2.9% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ADAM23. The exact immunogen used to generate this antibody is proprietary information. Database link O75077
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. |
Species Human | Dilution info - | Notes 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
May play a role in cell-cell and cell-matrix interactions. This is a non-catalytic metalloprotease-like protein.
Alternative names
MDC3, ADAM23, Disintegrin and metalloproteinase domain-containing protein 23, ADAM 23, MDC-3
Recommended products
Rabbit Polyclonal ADAM23 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ADAM23.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 2.9% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ADAM23. The exact immunogen used to generate this antibody is proprietary information. Database link O75077
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAM23 also known as A Disintegrin And Metalloprotease 23 is a member of the ADAM family of proteases. This protein has a mass of approximately 95 kDa. ADAM23 is expressed in several tissues but it has a significant presence in the brain. The protein mechanically functions by engaging in protease activities though it is uniquely categorized due to its role beyond traditional proteolytic functions. It interacts with surrounding cell-matrix elements through disintegrin domains.
- Biological function summary
ADAM23 plays a role in processes such as cell-cell adhesion and signal transduction. It aids in the regulation of synapse formation and maintenance in neuronal cells which is important for proper neural development and function. ADAM23 is not generally considered a part of large protein complexes but collaborates in the cellular environment impacting spatial cellular frameworks and growth signaling cascades.
- Pathways
Multiple mechanisms rely on ADAM23 especially those involved in cell signaling. The protein interacts with integrin pathways influencing cellular adhesion and migration dynamics. Furthermore it works closely with other members of the ADAM family such as ADAM22 in modulating signaling pathways critical for neuronal function and transmission.
- Associated diseases and disorders
ADAM23 has shown connections to neurological conditions notably epilepsy and some types of cancer. Disruptions or mutations in the ADAM23 gene have associated alterations in nervous system activity contributing to epilepsy pathogenesis. During cancer progression ADAM23 may interact with tumor-suppressing pathways in which its family member ADAM22 also participates. Understanding ADAM23's role in these diseases can lead to new insights into therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-ADAM23 antibody - Cytoplasmic domain (ab28302)
ICC/IF image of ab28302 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28302, 1 μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.
Western blot - Anti-ADAM23 antibody - Cytoplasmic domain (ab28302)
The bands observed at 75 kDa could potentially be a cleaved form of ADAM23 due to the presence of a signal peptide and propeptide.
All lanes: Western blot - Anti-ADAM23 antibody - Cytoplasmic domain (ab28302) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg
Lane 2: Rat Cortex Tissue Lysate at 10 µg
Lane 3: Human brain amygdala tissue lysate (alzheimer) - membrane extract (ab30118) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 33 kDa, 68 kDa, 75 kDa
Exposure time: 8min
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