Anti-ADAM23 antibody - Cytoplasmic domain

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ADAM23 antibody - Cytoplasmic domain (AB28302)

ICC/IF image of ab28302 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28302, 1 μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.

• WB

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Western blot - Anti-ADAM23 antibody - Cytoplasmic domain (AB28302)

The bands observed at 75 kDa could potentially be a cleaved form of ADAM23 due to the presence of a signal peptide and propeptide.

All lanes:

Western blot - Anti-ADAM23 antibody - Cytoplasmic domain (ab28302) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Rat Cortex Tissue Lysate at 10 µg

Lane 3:

Human brain amygdala tissue lysate (alzheimer) - membrane extract (<a href='/en-us/products/unavailable/human-brain-amygdala-tissue-lysate-alzheimer-membrane-extract-ab30118'>ab30118</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 92 kDa

Observed band size: 33 kDa,68 kDa,75 kDa

true

Exposure time: 8min