AB226459

Anti-ADAM9 antibody

Name: Anti-ADAM9 antibody (ab226459) | Abcam
Brand: Abcam Limited
SKU: AB226459
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal ADAM9 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ADAM9 aa 400-450.

View Alternative Names

KIAA0021, MCMP, MDC9, MLTNG, ADAM9, Disintegrin and metalloproteinase domain-containing protein 9, ADAM 9, Cellular disintegrin-related protein, Meltrin-gamma, Metalloprotease/disintegrin/cysteine-rich protein 9, Myeloma cell metalloproteinase

2 Images

Supplier Data

Immunoprecipitation - Anti-ADAM9 antibody (AB226459)

ADAM9 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using RIPA buffer; 1 mg for IP, 20% of IP loaded) with ab226459 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226459 at 0.4 μg/ml.

Lane 1 : ab226459 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-ADAM9 antibody (ab226459)

Predicted band size: 91 kDa

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Supplier Data

Western blot - Anti-ADAM9 antibody (AB226459)

All lanes:

Western blot - Anti-ADAM9 antibody (ab226459) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using NETN lysis buffer at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using RIPA lysis buffer at 50 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN lysis buffer at 50 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using NETN lysis buffer at 50 µg

Lane 5:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate prepared using NETN lysis buffer at 50 µg

Predicted band size: 91 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ADAM9 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

Q13443

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226459 was affinity purified using an epitope specific to ADAM9 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAM9 also known as Meltrin gamma is a member of the ADAM (a disintegrin and metalloproteinase) family characterized by both proteolytic and adhesive properties. The ADAM9 protein weighs approximately 84 kDa and is expressed in various tissues including the brain throat and lungs. It plays an important role in cellular processes such as cell-cell and cell-matrix interactions due to its disintegrin domain.

Biological function summary

ADAM9 participates in numerous essential cellular functions particularly in cell surface protein shedding and proteolytic activities. It is part of the protease complex involved in shedding epidermal growth factor receptor (EGFR) ligands. This action impacts cell signaling pathways that contribute to processes such as cell proliferation migration and invasion.

Pathways

Many studies link ADAM9 to pathways involving cell signal modulation and extracellular matrix remodeling. It participates in the EGFR signaling pathway significantly affecting cell behavior. ADAM9 interacts with other proteins such as integrins which facilitate changes in cell dynamics and communication.

Evidence implicates ADAM9 in several cancers including breast cancer and prostate cancer. It plays a role in cancer development by promoting cell invasion and metastasis. ADAM9 is also associated with Alzheimer's disease through interactions with proteins like APP (amyloid precursor protein) suggesting its involvement in neuronal degradation and plaque formation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Metalloprotease that cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as TEK, KDR, EPHB4, CD40, VCAM1 and CDH5. May mediate cell-cell, cell-matrix interactions and regulate the motility of cells via interactions with integrins.. Isoform 2. May act as alpha-secretase for amyloid precursor protein (APP).

See full target information ADAM9

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 19:133 PubMed39709463

2024

MLL1 promotes placental trophoblast ferroptosis and aggravates preeclampsia symptoms through epigenetic regulation of RBM15/TRIM72/ADAM9 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Li,Haining He,Zhenrong Zheng,Xiaolan Zhao

Biomolecules & biomedicine 24:505-519 PubMed37902450

2024

Silencing METTL14 alleviates liver injury in non-alcoholic fatty liver disease by regulating mitochondrial homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Jun Yan,Long Han,Zi-Lin Zou,Ai-Lei Xu

The Journal of international medical research 49:3000605211013548 PubMed34187214

2021

Long non-coding RNA (lncRNA) HOXB-AS3 promotes cell proliferation and inhibits apoptosis by regulating ADAM9 expression through targeting miR-498-5p in endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xing,Xianhua Sun,Feng Li,Xuan Jiang,Afang Jiang,Xiaofan Li,Ruiting Lv,Liwei Shao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADAM9 antibody

Immunoprecipitation - Anti-ADAM9 antibody (AB226459)

Western blot - Anti-ADAM9 antibody (AB226459)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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