AB39194

Anti-ADAMTS1 antibody

Name: Anti-ADAMTS1 antibody (ab39194) | Abcam
Brand: Abcam Limited
SKU: AB39194
Price: 565 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(15 Publications)

Rabbit Polyclonal ADAMTS1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human ADAMTS1.

View Alternative Names

KIAA1346, METH1, ADAMTS1, A disintegrin and metalloproteinase with thrombospondin motifs 1, ADAM-TS 1, ADAM-TS1, ADAMTS-1, METH-1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-ADAMTS1 antibody (AB39194)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ADAMTS1 antibody (AB39194)

ICC/IF image of ab39194 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39194, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human ADAMTS1. The exact immunogen used to generate this antibody is proprietary information.

Q9UHI8

Specificity

Ab39194 recognizes the zymogen of ADAMTS1, and the furin cleaved ADAMTS1, but not the shed form.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS1 short for a disintegrin and metalloproteinase with thrombospondin motifs 1 is an enzyme with a mass of around 110 kDa. Researchers often study this enzyme for its role in protein degradation and modification. ADAMTS1 is found in various tissues including the heart lungs and kidneys. This protein has several aliases sometimes referred to as ADAMTS-1 or ADAM-TS1. Its primary function involves processing extracellular matrix components such as aggrecan and versican which contribute to tissue remodeling.

Biological function summary

The influence of ADAMTS1 extends to various physiological and developmental processes. This enzyme participates in the modulation of angiogenesis inflammation and reproductive functions. It operates within a complex of metalloproteinases coordinating the breakdown of matrix proteins. Through these activities ADAMTS1 maintains tissue integrity and homeostasis. It particularly plays an essential role in the regulation of proteoglycans which are critical in cellular communication and structure.

Pathways

The actions of ADAMTS1 intersect within the extracellular matrix organization and integrin signaling pathways. These pathways are important for cell adhesion and migration contributing to tissue morphogenesis and repair. ADAMTS1 shows interaction with proteins like integrins and other matrix metalloproteinases establishing a network critical for cellular structural changes and signal transduction. These interactions ensure balanced tissue development and response to environmental changes.

The dysfunction of ADAMTS1 has links to conditions like osteoarthritis and cancer. In osteoarthritis inadequate regulation of matrix components by ADAMTS1 can lead to joint degradation. In cancer its dysregulation may influence tumor growth and metastasis. ADAMTS1 interacts with other proteins implicated in these diseases such as aggrecanases in osteoarthritis and integrins in cancer. These connections highlight the potential role of ADAMTS1 as a therapeutic target in managing these complex diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cleaves aggrecan, a cartilage proteoglycan, at the '1938-Glu-|-Leu-1939' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.

See full target information ADAMTS1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Histochemistry and cell biology 163:62 PubMed40461860

2025

ADAMTS-1, a multifunctional proteinase, in the uterus of both estrous cycle rats and ovariectomized rats can be regulated via hormones.

Applications

Unspecified application

Species

Unspecified reactive species

Tuba Parlak Ak,Mine Yaman,Ali Bayrakdar,Ozgur Bulmus

Nature communications 14:870 PubMed36797266

2023

Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2.

Applications

Unspecified application

Species

Unspecified reactive species

Karsten Nalbach,Martina Schifferer,Debjani Bhattacharya,Hung Ho-Xuan,Wei Chou Tseng,Luis A Williams,Alexandra Stolz,Stefan F Lichtenthaler,Zvulun Elazar,Christian Behrends

Molecules (Basel, Switzerland) 27: PubMed36144730

2022

Differential Expression and Localization of ADAMTS Proteinases in Proliferative Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Mohd Imtiaz Nawaz,Eef Allegaert,Mohammad Mairaj Siddiquei,Ajmal Ahmad,Priscilla Gikandi,Gert De Hertogh,Ghislain Opdenakker

Oral diseases 29:1184-1196 PubMed34874590

2021

Berberine regulates bone metabolism in apical periodontitis by remodelling the extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Yujia Cui,Jing Xie,Linyi Cai,Demao Zhang,Jianxun Sun,Xuedong Zhou

Cell & bioscience 11:155 PubMed34348778

2021

ADAMTS-1: a novel target gene of an estrogen-induced transcription factor, EGR1, critical for embryo implantation in the mouse uterus.

Applications

Unspecified application

Species

Unspecified reactive species

Mira Park,So Hee Park,Hyunsun Park,Hye-Ryun Kim,Hyunjung J Lim,Haengseok Song

Journal of histotechnology :1-12 PubMed33480322

2021

The alteration of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) in the knee joints of osteoarthritis mice.

Applications

Unspecified application

Species

Unspecified reactive species

Luling Wang,Caixia Pi,Jianxun Sun,Yujia Cui,Linyi Cai,Yuanchen Lan,Jinning Gu,Linfeng Liu,Geru Zhang,Lianyang Guo,Zhaowei Zhang,Qiang Guo,Liwei Zheng,Jing Xie,Demao Zhang,Xuedong Zhou

Genome biology 21:64 PubMed32160911

2020

Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Juri Kazakevych,Jérémy Denizot,Anke Liebert,Mariana Portovedo,Mia Mosavie,Payal Jain,Claudia Stellato,Claire Fraser,Renan Oliveira Corrêa,Marina Célestine,Raphaël Mattiuz,Hanneke Okkenhaug,J Ross Miller,Marco Aurélio Ramirez Vinolo,Marc Veldhoen,Patrick Varga-Weisz

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 52:1553-1568 PubMed31135123

2019

ADAMTS1 Promotes Adhesion to Extracellular Matrix Proteins and Predicts Prognosis in Early Stage Breast Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Izza A Tan,Kate Frewin,Carmela Ricciardelli,Darryl L Russell

Oncotarget 10:3559-3575 PubMed31191826

2019

Interleukin 1 is a key driver of inflammatory bowel disease-demonstration in a murine IL-1Ra knockout model.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha H Dosh,Nicola Jordan-Mahy,Christopher Sammon,Christine Le Maitre

Scientific reports 9:1812 PubMed30755679

2019

Long-term in vitro 3D hydrogel co-culture model of inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha H Dosh,Nicola Jordan-Mahy,Christopher Sammon,Christine L Le Maitre

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ADAMTS1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ADAMTS1 antibody (AB39194)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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