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AB249751

Anti-ADAMTS10 antibody [EPR11860] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ADAMTS10 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 10, ADAM-TS 10, ADAM-TS10, ADAMTS-10, ADAMTS10

1 Images
Western blot - Anti-ADAMTS10 antibody [EPR11860] - BSA and Azide free (AB249751)
  • WB

Supplier Data

Western blot - Anti-ADAMTS10 antibody [EPR11860] - BSA and Azide free (AB249751)

This data was developed using ab173290, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ADAMTS10 antibody [EPR11860] (<a href='/en-us/products/primary-antibodies/adamts10-antibody-epr11860-ab173290'>ab173290</a>) at 1/1000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Predicted band size: 121 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11860

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab249751 is the carrier-free version of ab173290.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS10 also referred to as A Disintegrin and Metalloproteinase with Thrombospondin Motifs 10 is a member of the ADAMTS family. This protein has an approximate molecular mass of 110 kDa. The ADAMTS10 protein functions mainly as a metalloprotease involved in the cleavage of extracellular matrix components affecting tissue remodeling and development processes. It is expressed in various tissues including the eyes and heart suggesting its broad role in different physiological contexts.
Biological function summary

ADAMTS10 plays a significant role in maintaining the structure of fibrillin-rich microfibrils which are essential components of the extracellular matrix. It is part of a larger complex involved in cell signaling and tissue integrity. The interaction with fibrillin suggests its involvement in connective tissue maintenance and elastogenesis which is important for normal cellular functions in eye and cardiac tissue.

Pathways

ADAMTS10 provides functionality within fibrillin and microfibril assembly pathways impacting processes like tissue morphogenesis. It associates closely with other matrix proteins like fibrillin-1 and ADAMTSL proteins in these pathways influencing the structural integrity and biomechanical properties of tissues. Additionally its regulation can affect Notch signaling pathways linking to cellular growth and differentiation processes.

ADAMTS10 mutations are linked to Weill-Marchesani syndrome a connective tissue disorder characterized by eye heart and skeletal abnormalities. The protein's activity also associates with glaucoma a condition involving elevated intraocular pressure leading to optic nerve damage. Researchers have noted a connection with LTBP2 (latent-transforming growth factor beta-binding protein 2) in these conditions suggesting that mutations or dysfunctions in this pathway can disrupt fibrillin functions and lead to disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues.
See full target information ADAMTS10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:8379962 PubMed34630853

2021

circ_0023461 Silencing Protects Cardiomyocytes from Hypoxia-Induced Dysfunction through Targeting miR-370-3p/PDE4D Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ren,Buying Li,Liqing Jiang,Zhiheng Liu,Fan Wu,Yi Zhang,Jincheng Liu,Weixun Duan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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