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AB45041

Anti-ADAMTS12 antibody

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(2 Publications)

Rabbit Polyclonal ADAMTS12 antibody. Suitable for WB and reacts with Mouse, African green monkey samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ADAMTS12.

View Alternative Names

UNQ1918/PRO4389, ADAMTS12, A disintegrin and metalloproteinase with thrombospondin motifs 12, ADAM-TS 12, ADAM-TS12, ADAMTS-12

1 Images
Western blot - Anti-ADAMTS12 antibody (AB45041)
  • WB

Unknown

Western blot - Anti-ADAMTS12 antibody (AB45041)

Full-length ADAMTS12 has been detected at 178 kDa by WB. However, study looking at ADAM-TS 12 expression in COS-7 cells (Gal et al. (2001) J. Biol. Chem. 276 : 17932), has detected an 83 kD COOH-terminal cleavage product.It is possible that the post-translation processing in COS-7 cells is different than that of other cells, that the epitope tag is cleaved during processing, or that the levels of the 83 kD product in other cells were below detection.

All lanes:

Western blot - Anti-ADAMTS12 antibody (ab45041) at 1/1000 dilution

Lane 1:

Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with TPA)

Lane 2:

Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with IL1-alpha)

Lane 3:

Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with TNF-alpha)

Predicted band size: 178 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, African green monkey

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ADAMTS12. The exact immunogen used to generate this antibody is proprietary information.

P58397

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS12 also recognized as A Disintegrin and Metalloproteinase with Thrombospondin Motifs 12 is a member of the ADAMTS family. It is a secreted enzyme with a mass of approximately 204 kDa. This protease is involved in extracellular matrix remodelling. The expression of ADAMTS12 occurs mainly in placental tissue and at lower levels in various other tissues including the lung and heart. It plays a role in processing protein substrates in the extracellular environment.
Biological function summary

The interactions with other matrix components allow ADAMTS12 to mediate matrix organization and degradation. It does not form part of a larger protein complex on its own but interacts with extracellular matrix proteins like aggrecan and versican. These interactions facilitate the breakdown of large proteoglycans aiding tissue morphogenesis and repair.

Pathways

ADAMTS12 participates in the extracellular matrix organization and degradation pathways. These pathways involve proteins such as aggrecanase and versicanase which ADAMTS12 closely relates to through its proteolytic activity. It plays a significant role in matrix remodelling which is essential for cellular migration and differentiation processes.

Alterations in ADAMTS12 expression link to osteoarthritis and cancer. In osteoarthritis increased ADAMTS12 activity contributes to cartilage degradation. In cancer its expression modifies cellular environments influencing tumor progression. It also interacts with matrix proteins like aggrecan in osteoarthritis and demonstrates associations with cancer-related proteins contributing to disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Metalloprotease that may play a role in the degradation of COMP. Also cleaves alpha-2 macroglobulin and aggregan. Has anti-tumorigenic properties.
See full target information ADAMTS12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108155 PubMed39761856

2025

Unveiling ADAMTS12: A key driver of bladder cancer progression via COL3A1-Mediated activation of the FAK/PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Hua Xiao,Li-Zhe Xu,Jin-Zhuo Ning,Fan Cheng

Comparative medicine 60:118-22 PubMed20412686

2010

The t(14,15) in mouse strain CBA/CaH-T(14;15)6Ca/J causes a break in the ADAMTS12 gene.

Applications

WB

Species

Mouse

Bengi Acar-Perk,Karen Bräutigam,Regina Grunewald,Andreas Schmutzler,Christian Schem,Norbert K Arnold,Walter Jonat,Jörg Weimer
View all publications
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Product promise

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