AB90786

Anti-ADAMTS13 antibody [20A5]

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(1 Publication)

Mouse Monoclonal ADAMTS13 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ADAMTS13.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

20A5

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ELISA, WB, ICC/IF

Immunogen

Recombinant Full Length Protein corresponding to Human ADAMTS13.

Epitope

ab90786 recognizes the central to C terminal TSP-1 repeats 2 to 5 of ADAMTS13 (amino acids 686-894).

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab90786 was filtered through a 0.2 µm filter.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS13 also known as von Willebrand factor-cleaving protease (VWFCP) is a zinc-containing metalloprotease with a molecular mass of approximately 190 kDa. This protein belongs to the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family and exhibits a complex structure with specific domains including a metalloprotease and a disintegrin-like domain. ADAMTS13 is produced mainly in the liver and circulates in the blood plasma. Research shows that it primarily facilitates the cleavage of von Willebrand factor (vWF) a large multimeric protein essential for blood clotting.

Biological function summary

ADAMTS13 plays a role in regulating the size and function of von Willebrand factor (vWF) ensuring proper hemostatic balance. The enzyme prevents the accumulation of ultra-large vWF multimers which can lead to spontaneous platelet aggregation and thrombus formation. Although ADAMTS13 acts independently its function is closely linked to the dynamics of vWF in response to vascular injury. Properly functioning ADAMTS13 aids in maintaining normal blood flow by preventing unnecessary clot formation in the bloodstream.

Pathways

ADAMTS13 is critical in the coagulation and hemostatic pathways. It operates by modulating the activity of vWF which plays an essential role in platelet adhesion and aggregation forming part of the coagulation cascade. A significant relationship exists between ADAMTS13 and vWF in these pathways as the protease controls vWF multimer size directly impacting clot formation. In the context of hemostatic balance ADAMTS13 intersects with factors like thrombin and fibrinogen which further contribute to clotting processes.

ADAMTS13 deficiency or dysfunction is associated with thrombotic thrombocytopenic purpura (TTP) a rare but severe blood disorder. In TTP the reduced activity of ADAMTS13 leads to an over-accumulation of ultra-large vWF multimers resulting in excessive platelet aggregation and microvascular thrombosis. Another related condition is atypical hemolytic uremic syndrome (aHUS) where abnormal ADAMTS13 activity might exacerbate the disease pathology. Both disorders highlight the critical need for balanced ADAMTS13 function in maintaining vascular health and highlight its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.

See full target information ADAMTS13

Alternative Names

C9orf8, UNQ6102/PRO20085, ADAMTS13, A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1394 PubMed39952917

2025

Matrix mechano-sensing at the invasive front induces a cytoskeletal and transcriptional memory supporting metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Maiques,Marta C Sallan,Roman Laddach,Pahini Pandya,Adrian Varela,Eva Crosas-Molist,Jaume Barcelo,Olivia Courbot,Yanbo Liu,Vittoria Graziani,Youssef Arafat,Joanne Sewell,Irene Rodriguez-Hernandez,Bruce Fanshawe,Yaiza Jung-Garcia,Paul Rc Imbert,Eloise M Grasset,Jean Albrengues,Maria Santacana,Anna Macià,Jordi Tarragona,Xavier Matias-Guiu,Rosa M Marti,Sophia Tsoka,Cedric Gaggioli,Jose L Orgaz,Gilbert O Fruhwirth,Fredrik Wallberg,Kai Betteridge,Constantino Carlos Reyes-Aldasoro,Syed Haider,Andrejs Braun,Sophia N Karagiannis,Alberto Elosegui-Artola,Victoria Sanz-Moreno

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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