AB185722

Anti-ADAMTS4 antibody

Name: Anti-ADAMTS4 antibody. Rabbit polyclonal (ab185722) | Abcam
Brand: Abcam Limited
SKU: AB185722
Price: 470 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(88 Publications)

Rabbit Polyclonal ADAMTS4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 88 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADAMTS4 aa 50-350.

View Alternative Names

KIAA0688, UNQ769/PRO1563, ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4, ADAM-TS 4, ADAM-TS4, ADAMTS-4, ADMP-1, Aggrecanase-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-ADAMTS4 antibody (AB185722)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADAMTS4 antibody (AB185722)

Immunofluorescence staining of C6 cells stained for ADAMTS4 with ab185722 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

Supplier Data

Western blot - Anti-ADAMTS4 antibody (AB185722)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-ADAMTS4 antibody (ab185722) at 1/3000 dilution

Lane 1:

SH-SY5Y cell lysate at 25 µg

Lane 2:

A-549 cell lysate at 25 µg

Lane 3:

SKOV3 cell lysate at 25 µg

Lane 4:

293T cell lysate at 25 µg

Lane 5:

Mouse brain tissue lysate at 25 µg

Lane 6:

Rat brain tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 90 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ADAMTS4 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

O75173

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS4 also known as aggrecanase-1 is a protein responsible for extracellular matrix degradation. It weighs approximately 90 kDa. This metalloproteinase is expressed in various tissues including cartilage and synovial tissue. It cleaves aggrecan a core protein of the proteoglycan into fragments impacting the integrity and function of the cartilage matrix. It is secreted as a latent enzyme and undergoes activation in the extracellular space.

Biological function summary

ADAMTS4 influences tissue remodeling through the breakdown of cartilage. It contributes to the disassembly of aggrecan and other matrix components influencing extracellular matrix turnover. As part of a group of similar enzymes it plays a role in proteolytic processes important for normal tissue maintenance and repair. Its actions affect the balance between matrix synthesis and degradation which is important for maintaining tissue homeostasis.

Pathways

ADAMTS4 participates in pathways such as the inflammation-related cytokine signaling and the degradation of the extracellular matrix. This protein interacts closely with ADAMTS5 contributing to matrix degradation and modulation of inflammatory processes. In these pathways the balanced activity of ADAMTS4 is essential for proper cartilage function and the resolution of inflammation in tissues.

ADAMTS4 is notably linked to osteoarthritis and rheumatoid arthritis. These conditions involve excessive cartilage breakdown where ADAMTS4 and ADAMTS5 contribute significantly to disease progression through aggrecan degradation. Understanding the activity and regulation of ADAMTS4 offers insights into potential therapeutic strategies for managing cartilage degeneration and inflammatory joint diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

See full target information ADAMTS4

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Publications (88)

Recent publications for all applications. Explore the full list and refine your search

Journal of pharmaceutical analysis 15:101092 PubMed40496065

2025

Screen of FDA-approved drug library identifies vitamin K as anti-ferroptotic drug for osteoarthritis therapy through Gas6.

Applications

Unspecified application

Species

Unspecified reactive species

Yifeng Shi,Sunlong Li,Shuhao Zhang,Caiyu Yu,Jiansen Miao,Shu Yang,Yan Chen,Yuxuan Zhu,Xiaoxiao Huang,Chencheng Zhou,Hongwei Ouyang,Xiaolei Zhang,Xiangyang Wang

Journal of orthopaedic translation 52:126-137 PubMed40291636

2025

The mitochondrial protectant SS31 optimized decellularized Wharton's jelly scaffold improves allogeneic chondrocyte implantation-mediated articular cartilage repair.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wang,Hao Li,Fakai Li,Yongkang Yang,Ziheng Xu,Tianze Gao,Runmeng Li,Ruiyang Zhang,Yuhao Mu,Zheng Guo,Quanyi Guo,Shuyun Liu

International journal of nanomedicine 20:505-521 PubMed39830158

2025

Microfluidic Synthesis of miR-200c-3p Lipid Nanoparticles: Targeting ZEB2 to Alleviate Chondrocyte Damage in Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zheng,Tong Chen,Kaiyuan Yang,Guangrong Yin,Yang Chen,Jianchao Gui,Chao Xu,Songwei Lv

Journal of translational medicine 22:741 PubMed39107784

2024

Pulsed electromagnetic fields potentiate bone marrow mesenchymal stem cell chondrogenesis by regulating the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kangping Song,Jing Hu,Ming Yang,Yong Xia,Chengqi He,Yonghong Yang,Siyi Zhu

Annals of translational medicine 12:43 PubMed38911554

2024

Removal of the infrapatellar fat pad and associated synovium benefits female guinea pigs in the Dunkin Hartley model of idiopathic osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam F Afzali,Madeline M Sykes,Lindsey H Burton,Kayley M Patton,Koryn R Lee,Cassie Seebart,Nicole Vigon,Ryan Ek,Gerardo E Narez,Angela J Marolf,Katie J Sikes,Tammy L Haut Donahue,Kelly S Santangelo

Molecular therapy : the journal of the American Society of Gene Therapy 32:2549-2562 PubMed38879753

2024

CRISPR-mediated Sox9 activation and RelA inhibition enhance cell therapy for osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Zhao,Yumei Lai,Hongli Jiao,Jun Li,Ke Lu,Jian Huang

Journal of orthopaedic surgery and research 19:352 PubMed38877549

2024

Parathyroid hormone (1-34) retards the lumbar facet joint degeneration and activates Wnt/β-catenin signaling pathway in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Gou,Hetong Li,Xun Sun,Desheng Chen,Faming Tian

Open life sciences 19:20220878 PubMed38840893

2024

Role of Embinin in the reabsorption of nucleus pulposus in lumbar disc herniation: Promotion of nucleus pulposus neovascularization and apoptosis of nucleus pulposus cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Meng,Wei Liu,Haifeng Liu,Chengwei Yu

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 42:1933-1942 PubMed38520666

2024

Cardamonin inhibits osteogenic differentiation by downregulating Wnt/beta-catenin signaling and alleviates subchondral osteosclerosis in osteoarthritic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fanding Meng,Pengchong Zhu,Xiaoli Ren,Limei Wang,Dong Ding,Jiangbo Yan,Ying Zhang,Shang-You Yang,Bin Ning

Acta biochimica et biophysica Sinica 56:776-788 PubMed38495003

2024

Maslinic acid alleviates intervertebral disc degeneration by inhibiting the PI3K/AKT and NF-κB signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yichen Que,Chipiu Wong,Jincheng Qiu,Wenjie Gao,Youxi Lin,Hang Zhou,Bo Gao,Pengfei Li,Zhihuai Deng,Huihong Shi,Wenjun Hu,Song Liu,Yan Peng,Peiqiang Su,Caixia Xu,Anjing Liang,Xianjian Qiu,Dongsheng Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADAMTS4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ADAMTS4 antibody (AB185722)

Western blot - Anti-ADAMTS4 antibody (AB185722)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (88)

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