AB182795

Anti-ADAMTS5 antibody

Name: Anti-ADAMTS5 antibody (ab182795) | Abcam
Brand: Abcam Limited
SKU: AB182795
Price: 535 USD
Availability: InStock

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(16 Publications)

Rabbit Polyclonal ADAMTS5 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human ADAMTS5 aa 500-550.

View Alternative Names

ADAMTS11, ADMP2, ADAMTS5, A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAM-TS 5, ADAM-TS5, ADAMTS-5, A disintegrin and metalloproteinase with thrombospondin motifs 11, ADMP-2, Aggrecanase-2, ADAM-TS 11, ADAMTS-11

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (AB182795)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (AB182795)

Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling ADAMTS5 with ab182795 at 1/10. Image on the right is treated with the synthetic peptide.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (AB182795)

Immunohistochemical analysis of paraffin embedded human lung tissue labeling ADAMTS5 with ab182795 at 1/50 dilution.

The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (AB182795)

Immunohistochemical analysis of paraffin embedded human kidney tissue labeling ADAMTS5 with ab182795 at 1/50 dilution.

Supplier Data

Western blot - Anti-ADAMTS5 antibody (AB182795)

All lanes:

Western blot - Anti-ADAMTS5 antibody (ab182795) at 1/650 dilution

All lanes:

Human fetal brain tissue at 40 µg

Secondary

All lanes:

Goat anti Rabbit IgG at 1/3000 dilution

Predicted band size: 102 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ADAMTS5 aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

Q9UNA0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/50", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS5 also known as aggrecanase-2 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Its approximate mass is 120 kDa. This enzyme functions primarily as a metalloprotease cleaving proteoglycans such as aggrecan thereby playing a significant role in the degradation of extracellular matrix components. ADAMTS5 is widely expressed in tissues such as cartilage. It is integral for maintaining tissue homeostasis and flexibility within the joint structures.

Biological function summary

ADAMTS5 significantly contributes to the dynamic remodeling of the extracellular matrix. It operates without forming complexes with other proteins and functions independently. By cleaving aggrecan it regulates cartilage integrity balancing synthesis and breakdown of matrix constituents. This balance is essential for facilitating normal cartilage function and enables joints to resist load and stress.

Pathways

Current understanding highlights that ADAMTS5 is critical within pathways like cartilage catabolism and inflammation response. ADAMTS5 activity initiates extracellular matrix remodeling by interacting with proteins such as aggrecan and collagen II. It plays a role in precise regulatory processes that manage cartilage homeostasis and maintains stability in structural integrity through its catabolic actions.

ADAMTS5 has strong links to osteoarthritis and rheumatoid arthritis. Pathological overexpression or dysregulated activity of ADAMTS5 can accelerate cartilage degradation within these conditions. ADAMTS5 interacts with proteins involved in inflammatory pathways and its activity correlates with inflammatory cytokines. Understanding its role is important for designing therapies aimed at inhibiting its excessive action and developing strategies to slow down the disease progression in arthritis patients.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN (PubMed : 16133547, PubMed : 18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration (By similarity). Participates in development of brown adipose tissue and browning of white adipose tissue (By similarity). Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.

See full target information ADAMTS5

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Stem cells international 2025:5091529 PubMed40476182

2025

miR-1275 Delivered via Mesenchymal Stem Cell-Derived Extracellular Vesicles Regulates ER-Phagy Through AXIN2 in Nucleus Pulposus Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwu Dong,Hailong Zhang,Wenwei Yang,Keliang Huang,Xin Zhang,Lianxiang Xing,Ying Zhang,Kewen Zhao

Scientific reports 15:18740 PubMed40437041

2025

Dihydrotestosterone and 17β-estradiol modulate TMJ osteoarthritis development and reveal sex-specific differences in pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Takuma Tomura,Takenobu Ishii,Norio Kasahara,Yasushi Nishii

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2501046 PubMed40279639

2025

Mechanical Load-Induced Upregulation of Talin2 through Non-Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhen Huang,Heng Sun,Haojie Chen,Xiangpeng Wang,Junduo Zhao,Yang Jiao,Hongyi Zhou,Haoyu Cai,Jiafeng Dai,Xuan Huang,Weiyun Chen,Jianxiong Shen

Frontiers in immunology 15:1513196 PubMed39902039

2025

Inflammatory biomarkers and therapeutic potential of milk exosome-mediated CCL7 siRNA in murine intestinal ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

WenDong Chen,WenPing Xu,Li Ma,Chun Bi,MeiXu Yang,Wei Yang

Clinical and translational medicine 15:e70118 PubMed39731281

2024

The antiarthritic effect of CBR-470-1 in hypoxic environment is to increase the level of NOD-like receptor family pyrin domain containing 3 ubiquitination by decreasing phosphoglycerate kinase 1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ao Duan,Zemeng Ma,Xiaolong Shao,Zhencheng Xiong,Chaoyi Zhang,Wenzheng Liu,Guanglin Wang,Shouye Hu,Wei Lin

Phytotherapy research : PTR 37:5205-5222 PubMed37527970

2023

Ginkgolide K delays the progression of osteoarthritis by regulating YAP to promote the formation of cartilage extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Ruizhou Liu,Zhendong Liu,Hui Chen,Shiping He,Shihan Wang,Jihang Dai,Xiaolei Li

Scientific reports 12:15968 PubMed36153352

2022

FGF receptor inhibitor BGJ398 partially rescues osteoarthritis-like phenotype in older high molecular weight FGF2 transgenic mice via multiple mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Marja M Hurley,J Douglas Coffin,Thomas Doetschman,Christina Valera,Kai Clarke,Liping Xiao

Stem cell research & therapy 13:322 PubMed35842714

2022

Extracellular vesicles derived from human umbilical cord mesenchymal stem cells alleviate osteoarthritis of the knee in mice model by interacting with METTL3 to reduce m6A of NLRP3 in macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhou,Xun Shen,Chen Yan,Wu Xiong,Zemeng Ma,Zhenggang Tan,Jinwen Wang,Yao Li,Jiuxiang Liu,Ao Duan,Feng Liu

BMC musculoskeletal disorders 23:583 PubMed35710352

2022

Hypoxia-inducible factor expression is related to apoptosis and cartilage degradation in temporomandibular joint osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhang,Yu Hu,Zihan Wang,Xuelian Wu,Chun Yang,Hefeng Yang

Experimental and therapeutic medicine 23:207 PubMed35126710

2022

Col9a2 gene deletion accelerates the degeneration of intervertebral discs.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Xu,Rui Dong,Qinghe Zeng,Liang Fang,Qinwen Ge,Chenjie Xia,Peng Zhang,Shuaijie Lv,Zhen Zou,Pinger Wang,Ju Li,Hongfeng Ruan,Songfeng Hu,Chengliang Wu,Hongting Jin,Peijian Tong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ADAMTS5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (AB182795)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (AB182795)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (AB182795)

Western blot - Anti-ADAMTS5 antibody (AB182795)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise