AB231595

Anti-ADAMTS5 antibody

Name: Anti-ADAMTS5 antibody (ab231595) | Abcam
Brand: Abcam Limited
SKU: AB231595
Price: 535 USD
Availability: InStock

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(11 Publications)

Rabbit Polyclonal ADAMTS5 antibody. Suitable for WB, IHC-P and reacts with Recombinant full length protein - Rat, Human, Rat samples. Cited in 11 publications.

View Alternative Names

ADAMTS11, ADMP2, ADAMTS5, A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAM-TS 5, ADAM-TS5, ADAMTS-5, A disintegrin and metalloproteinase with thrombospondin motifs 11, ADMP-2, Aggrecanase-2, ADAM-TS 11, ADAMTS-11

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (AB231595)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (AB231595)

Formalin-fixed, paraffin-embedded rat ovary tissue stained for ADAMST5 using ab231595 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-ADAMTS5 antibody (AB231595)

All lanes:

Western blot - Anti-ADAMTS5 antibody (ab231595) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 102 kDa

false

Supplier Data

Western blot - Anti-ADAMTS5 antibody (AB231595)

All lanes:

Western blot - Anti-ADAMTS5 antibody (ab231595) at 2 µg/mL

All lanes:

Recombinant rat ADAMTS5 protein

Predicted band size: 102 kDa

false

Supplier Data

Western blot - Anti-ADAMTS5 antibody (AB231595)

All lanes:

Western blot - Anti-ADAMTS5 antibody (ab231595) at 2 µg/mL

All lanes:

A549 (Human lung carcinoma cell line) cell lysate

Predicted band size: 102 kDa,103 kDa,42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Recombinant full length protein - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS5 also known as aggrecanase-2 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Its approximate mass is 120 kDa. This enzyme functions primarily as a metalloprotease cleaving proteoglycans such as aggrecan thereby playing a significant role in the degradation of extracellular matrix components. ADAMTS5 is widely expressed in tissues such as cartilage. It is integral for maintaining tissue homeostasis and flexibility within the joint structures.

Biological function summary

ADAMTS5 significantly contributes to the dynamic remodeling of the extracellular matrix. It operates without forming complexes with other proteins and functions independently. By cleaving aggrecan it regulates cartilage integrity balancing synthesis and breakdown of matrix constituents. This balance is essential for facilitating normal cartilage function and enables joints to resist load and stress.

Pathways

Current understanding highlights that ADAMTS5 is critical within pathways like cartilage catabolism and inflammation response. ADAMTS5 activity initiates extracellular matrix remodeling by interacting with proteins such as aggrecan and collagen II. It plays a role in precise regulatory processes that manage cartilage homeostasis and maintains stability in structural integrity through its catabolic actions.

ADAMTS5 has strong links to osteoarthritis and rheumatoid arthritis. Pathological overexpression or dysregulated activity of ADAMTS5 can accelerate cartilage degradation within these conditions. ADAMTS5 interacts with proteins involved in inflammatory pathways and its activity correlates with inflammatory cytokines. Understanding its role is important for designing therapies aimed at inhibiting its excessive action and developing strategies to slow down the disease progression in arthritis patients.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN (PubMed : 16133547, PubMed : 18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration (By similarity). Participates in development of brown adipose tissue and browning of white adipose tissue (By similarity). Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.

See full target information ADAMTS5

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1577234 PubMed40475787

2025

Thrombospondin-1 mitigates osteoarthritis progression by inhibiting mechanical stress-induced chondrocyte ferroptosis via the integrin/YAP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoyi Wang,Xiaocong Zhou,Fujian Zhang,Haoxin Zhai,Yuanqiang Zhang,Yongyuan Guo

Journal of orthopaedic surgery and research 20:441 PubMed40319261

2025

Obacunone acts as a histone deacetylase 1 inhibitor to limit p38MAPK signaling and alleviate osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Gao,Ke Wang,Chao Shi,Yang Gao,De-Qian Kong

Journal of inflammation research 18:5293-5309 PubMed40264591

2025

Gubi Decoction Ameliorates Porous Cartilage Endplate in an Intervertebral Disc Degeneration Model Mouse Through Inhibition of NF-κB Activity and Pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sai Yao,Yanan Li,Hongfeng Ruan,Lianguo Wu,Hanbing Zeng

Advanced biology 9:e2400685 PubMed40197728

2025

Phytomolecule Epimedin C Mitigates Cartilage Extracellular Matrix Degradation and Osteoarthritis Progression in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyao Yang,Xiangbo Meng,Jiming Li,Huijuan Cao,Ling Li,Cuishan Huang,Yingchao Wang,Wakam Chang,Sibylle Grad,Zhen Li,Ling Qin,Xinluan Wang

Nature communications 15:9170 PubMed39448593

2024

IGF1 drives Wnt-induced joint damage and is a potential therapeutic target for osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Escribano-Núñez,Frederique M F Cornelis,Astrid De Roover,An Sermon,Frédéric Cailotto,Rik J Lories,Silvia Monteagudo

Bioactive materials 40:624-633 PubMed39247402

2024

Promoting chondrogenesis by targeted delivery to the degenerating cartilage in early treatment of osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiang Fei,Xiaojing Li,Zhongyang Lv,Zizheng Liu,Ya Xie,Jiaqi Chen,Weitong Li,Xiyu Liu,Hu Guo,Huan Liu,Zhaofeng Zhang,Xunhao Wang,Jingjing Fan,Chunqing Hu,Xiaoyu Jin,Ruiyang Jiang,Nuo Xu,Jiang Xia,Yang Li,Dongquan Shi

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 72:95-108 PubMed38213081

2024

General Control Non-derepressible 2 Alleviates Cartilage Degeneration and Inhibits NLRP3 Inflammasome Activation in Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Long Fang,Zhengyu Wang,Jisong Liu,Yongjie Lin,Wei Hao

Aging 15:13693-13709 PubMed38019477

2023

The antioxidant Glycitin protects against intervertebral disc degeneration through antagonizing inflammation and oxidative stress in nucleus pulposus cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhao,Yanpei Li,Xiang Cheng,Hui Wei,Peng Li,Lixia Fan,Kaiwen Liu,Shuai Zhang,Hao Wang

Bioengineering (Basel, Switzerland) 10: PubMed37760097

2023

Early Degenerative Changes in a Spontaneous Osteoarthritis Model Assessed by Nanoindentation.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Davis,Jurgita Zekonyte,Aikaterina Karali,Marta Roldo,Gordon Blunn

Pharmaceutical biology 60:427-436 PubMed35175176

2022

Cyanidin attenuates the apoptosis of rat nucleus pulposus cells and the degeneration of intervertebral disc via the JAK2/STAT3 signal pathway and .

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Bai,Meichao Jiang,Jie Wang,Shuai Yang,Zhiwei Liu,Hongxin Zhang,Xiaojuan Zhu

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADAMTS5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (AB231595)

Western blot - Anti-ADAMTS5 antibody (AB231595)

Western blot - Anti-ADAMTS5 antibody (AB231595)

Western blot - Anti-ADAMTS5 antibody (AB231595)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise