Rabbit Polyclonal ADAMTS5 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADAMTS5 aa 400-500.
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- Nature 612:546-5542022A plant-derived natural photosynthetic system for improving cell anabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengfei Chen,Xin Liu,Chenhui Gu,Peiyu Zhong,Nan Song,Mobai Li,Zhanqiu Dai,Xiangqian Fang,Zhaoming Liu,Jianfeng Zhang,Ruikang Tang,Shunwu Fan,Xianfeng LinPubMed 36477541
- Genome biology 21:642020Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuri Kazakevych et. al.PubMed 32160911
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ADAMTS5 aa 400-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNA0
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Cow | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
Target data
Function
Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN (PubMed:16133547, PubMed:18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration (By similarity). Participates in development of brown adipose tissue and browning of white adipose tissue (By similarity). Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.
Alternative names
ADAMTS11, ADMP2, ADAMTS5, A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAM-TS 5, ADAM-TS5, ADAMTS-5, A disintegrin and metalloproteinase with thrombospondin motifs 11, ADMP-2, Aggrecanase-2, ADAM-TS 11, ADAMTS-11
Recommended products
Rabbit Polyclonal ADAMTS5 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ADAMTS5 aa 400-500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ADAMTS5 aa 400-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNA0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAMTS5 also known as aggrecanase-2 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Its approximate mass is 120 kDa. This enzyme functions primarily as a metalloprotease cleaving proteoglycans such as aggrecan thereby playing a significant role in the degradation of extracellular matrix components. ADAMTS5 is widely expressed in tissues such as cartilage. It is integral for maintaining tissue homeostasis and flexibility within the joint structures.
- Biological function summary
ADAMTS5 significantly contributes to the dynamic remodeling of the extracellular matrix. It operates without forming complexes with other proteins and functions independently. By cleaving aggrecan it regulates cartilage integrity balancing synthesis and breakdown of matrix constituents. This balance is essential for facilitating normal cartilage function and enables joints to resist load and stress.
- Pathways
Current understanding highlights that ADAMTS5 is critical within pathways like cartilage catabolism and inflammation response. ADAMTS5 activity initiates extracellular matrix remodeling by interacting with proteins such as aggrecan and collagen II. It plays a role in precise regulatory processes that manage cartilage homeostasis and maintains stability in structural integrity through its catabolic actions.
- Associated diseases and disorders
ADAMTS5 has strong links to osteoarthritis and rheumatoid arthritis. Pathological overexpression or dysregulated activity of ADAMTS5 can accelerate cartilage degradation within these conditions. ADAMTS5 interacts with proteins involved in inflammatory pathways and its activity correlates with inflammatory cytokines. Understanding its role is important for designing therapies aimed at inhibiting its excessive action and developing strategies to slow down the disease progression in arthritis patients.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-ADAMTS5 antibody (ab246975)
PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for ADAMST5 (green) using ab246975 at 4 μg/ml in ICC/IF.
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