AB41037

Anti-ADAMTS5 antibody

Name: Anti-ADAMTS5 antibody (ab41037) | Abcam
Brand: Abcam Limited
SKU: AB41037
Price: 410 USD
Availability: InStock
Rating: 4 (9 reviews)

(9 Reviews)

(285 Publications)

Anti-ADAMTS5 antibody (ab41037) is a rabbit polyclonal antibody detecting ADAMTS5 in Western Blot. Suitable for Human, Mouse, Rat.

- Over 190 publications
- Trusted since 2007

View Alternative Names

ADAMTS11, ADMP2, ADAMTS5, A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAM-TS 5, ADAM-TS5, ADAMTS-5, A disintegrin and metalloproteinase with thrombospondin motifs 11, ADMP-2, Aggrecanase-2, ADAM-TS 11, ADAMTS-11

3 Images

Project

Western blot - Anti-ADAMTS5 antibody (AB41037)

ab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.

All lanes:

Western blot - Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 4:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 5:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 6:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 7:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 8:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 9:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Lane 10:

Liver (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 102 kDa

Observed band size: 73 kDa

false

Project

Western blot - Anti-ADAMTS5 antibody (AB41037)

ab41037 appears to detect a 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. The doublet observed in human uterus lysate is possibly due to glycosylation.

All lanes:

Western blot - Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution

Lane 1:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 10 µg

Lane 2:

Western blot - Mouse placenta normal tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-placenta-normal-tissue-lysate-total-protein-ab29139'>ab29139</a>) at 10 µg

Lane 3:

Uterus (Human) Whole Cell Lysate - normal tissue at 10 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 102 kDa

Observed band size: 27 kDa,73 kDa

false

Lab

Western blot - Anti-ADAMTS5 antibody (AB41037)

ab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.

All lanes:

Western blot - Anti-ADAMTS5 antibody (ab41037) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 102 kDa

Observed band size: 55 kDa,73 kDa

true

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250", "WB-species-notes": "ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250", "WB-species-notes": "ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250", "WB-species-notes": "ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase." } } }

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Product details

What is this antibody validated in?
Anti-ADAMTS5 antibody (ab41037) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of ADAMTS5?
Anti-ADAMTS5 (ab41037) specifically detects a band for ADAMTS5 (UniProt: Q9UNA0) at a molecular weight of 102kDa.

Trusted by the scientific community
Anti-ADAMTS5 (ab41037) was first used in a scientific publication in 2007 and has been cited over 190 times in peer-reviewed journals.

Reviewed by scientists
Anti-ADAMTS5 (ab41037) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAMTS5 also known as aggrecanase-2 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Its approximate mass is 120 kDa. This enzyme functions primarily as a metalloprotease cleaving proteoglycans such as aggrecan thereby playing a significant role in the degradation of extracellular matrix components. ADAMTS5 is widely expressed in tissues such as cartilage. It is integral for maintaining tissue homeostasis and flexibility within the joint structures.

Biological function summary

ADAMTS5 significantly contributes to the dynamic remodeling of the extracellular matrix. It operates without forming complexes with other proteins and functions independently. By cleaving aggrecan it regulates cartilage integrity balancing synthesis and breakdown of matrix constituents. This balance is essential for facilitating normal cartilage function and enables joints to resist load and stress.

Pathways

Current understanding highlights that ADAMTS5 is critical within pathways like cartilage catabolism and inflammation response. ADAMTS5 activity initiates extracellular matrix remodeling by interacting with proteins such as aggrecan and collagen II. It plays a role in precise regulatory processes that manage cartilage homeostasis and maintains stability in structural integrity through its catabolic actions.

ADAMTS5 has strong links to osteoarthritis and rheumatoid arthritis. Pathological overexpression or dysregulated activity of ADAMTS5 can accelerate cartilage degradation within these conditions. ADAMTS5 interacts with proteins involved in inflammatory pathways and its activity correlates with inflammatory cytokines. Understanding its role is important for designing therapies aimed at inhibiting its excessive action and developing strategies to slow down the disease progression in arthritis patients.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN (PubMed : 16133547, PubMed : 18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration (By similarity). Participates in development of brown adipose tissue and browning of white adipose tissue (By similarity). Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.

See full target information ADAMTS5

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Publications (285)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology :18995 PubMed41017373

2025

UCHL1 enhances transcription by stabilizing FOXO1 through deubiquitination in knee osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Jiawei Lu,Chonghao Gu,Zikang Xie,Zhongyu Xia,Bingqing Guo,Tao Jiang

Aging 17:2369-2391 PubMed40932696

2025

overexpression induces early onset of intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Takanori Fukunaga,Martha Elena Diaz-Hernandez,John G Heller,Changli Zhang,Hicham Drissi

Molecular medicine reports 32: PubMed40849812

2025

Reg3β promotes chondrocyte proliferation and ECM metabolism during acetabular roof remodeling in a rat model of DDH‑induced residual dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Mengxia Li,Wenxuan Wang,Weiguo Sun,Wen Zhen,Qian Sun,Bo Ning,Guanghao Su,Xiaodong Wang

Journal of cellular and molecular medicine 29:e70734 PubMed40745673

2025

The Mechanosensitive Ion Channel Piezo1 Regulates Chondrocyte Homeostasis Through the PI3K/AKT/mTORC1 Pathway in Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Han,Fangyan Cheng,Xinyan Li,Jiahao Yu,Guimiao Li,Wei Chen,Jiaxue Zhang,Chen Feng,Yingze Zhang,Juan Wang

Materials today. Bio 33:102103 PubMed40704013

2025

Dual-functional ROS-responsive hydrogel alleviates temporomandibular joint osteoarthritis by enhancing cartilage repair and mitigating inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwen Kuang,Bingqiang Hua,Xiaoping Ye,Yujin Zhao,Meng Yu,Xianwen Liu

Journal of orthopaedic translation 52:387-403 PubMed40476065

2025

Vitamins D and K jointly protect against osteoarthritis via regulating OSCAR during osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhao,Qianhua Ou,Hong Huang,Delong Li,Jianmao Chen,Song Xue,Zuoqing Zhou,Guangfeng Ruan,Changhai Ding

BMC musculoskeletal disorders 26:538 PubMed40450237

2025

Effect of intraarticular human umbilical cord mesenchymal stem cells transplantation on cartilage degradation and matrix metalloproteinases in OA rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqun Li,Qipeng Han,Shayan Chen,Wenlong Huang,Yanqing Chen,Zhihuang Wu,Fengming Tan,Muyun Liu,Jiangying Zou

Nature communications 16:4344 PubMed40346121

2025

Streamlined metal-based hydrogel facilitates stem cell differentiation, extracellular matrix homeostasis and cartilage repair in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Li,Zhiyuan Shi,Huaqing Jing,Yunsheng Dou,Xinyi Liu,Mengyao Zhang,Zitong Qiu,Zbynek Heger,Nan Li

Journal of oral & facial pain and headache 39:196-203 PubMed40129438

2025

Loss of SCRG1 in chondrocytes inhibits osteoarthritis by promoting autophagy activity in the temporomandibular joint through inhibition of neurokine receptors.

Applications

Unspecified application

Species

Unspecified reactive species

JiaJun Zhang,LePing Yuan,YanYan Zhang,HaoYang Jin,YeKe Zhao,XiaoKe Zeng,YanHui Zou,KeYu Wang,Xin Nie

Journal of inflammation research 18:4233-4250 PubMed40129871

2025

Systemic Lupus Erythematosus Stimulates Chondrocyte Pyroptosis to Aggravate Arthritis via Suppression of NRF-2/KEAP-1 and NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuchao Shen,Xuliang Fang,Helou Zhang,Tingting Lang,Fangda Fu,Yu Du,Taotao Xu,Hongting Jin,Peijian Tong,Chengliang Wu,Changfeng Hu,Hongfeng Ruan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ADAMTS5 antibody

Western blot - Anti-ADAMTS5 antibody (AB41037)

Western blot - Anti-ADAMTS5 antibody (AB41037)

Western blot - Anti-ADAMTS5 antibody (AB41037)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (285)

Product promise