Anti-ADAMTS5 antibody (ab41037) is a rabbit polyclonal antibody detecting ADAMTS5 in Western Blot. Suitable for Human, Mouse, Rat.
- Over 190 publications
- Trusted since 2007
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- Frontiers in bioengineering and biotechnology 11:13014302023Liquid platelet-rich fibrin produced via horizontal centrifugation decreases the inflammatory response and promotes chondrocyte regeneration .Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuimin Li,Ting Xia,Hao Zeng,Yun Qiu,Yan Wei,Yihong Cheng,Yulan Wang,Xiaoxin Zhang,Jin Ke,Richard Miron,Qing HePubMed 38144541
- Journal of orthopaedic surgery and research 18:9162023Inhibition of miR-96-5p alleviates intervertebral disc degeneration by regulating the peroxisome proliferator-activated receptor γ/nuclear factor-kappaB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXusheng Li,Qian Hou,Wenqi Yuan,Xuehua Zhan,Haifeng YuanPubMed 38041147
- Arthritis research & therapy 25:2302023Resatorvid alleviates experimental inflammatory TMJOA by restraining chondrocyte pyroptosis and synovial inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Liu,Huimin Li,Yaping Feng,Huilin Guo,Yingjie Li,Jin Ke,Xing LongPubMed 38031141
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e233032023Macrophage migration inhibitory factor (MIF) promotes intervertebral disc degeneration through the NF-κB pathway, and the MIF inhibitor CPSI-1306 alleviates intervertebral disc degeneration in a mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYejin Zhang,Lin Zheng,Jiawei Fang,Kainan Ni,Xingyu Hu,Lin Ye,Hehuan Lai,Tao Yang,Zhenzhong Chen,Dengwei HePubMed 37983963
- Journal of orthopaedic surgery and research 18:7842023PLXNC1 interference alleviates the inflammatory injury, apoptosis and extracellular matrix degradation of IL-1β-exposed chondrocytes via suppressing GRP78 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Meng,Lingwei Mao,Qinyi Jiang,Jishan Yuan,Linjuan Liu,Lei WangPubMed 37853395
- Journal of nanobiotechnology 21:3502023Programmable DNA hydrogel provides suitable microenvironment for enhancing autophagy-based therapies in intervertebral disc degeneration treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Qingxin,Jiang Kai,Zheng Dandan,Jin Linyu,Chen Xiuyuan,Feng Yubo,Wang Kun,Han Yingchao,Chen Hao,Song Jie,Chen Zhi,Shen HongxingPubMed 37759249
- International immunopharmacology 124:1109482023PP2 alleviates the progression of osteoarthritis by inhibiting Wnt/β-catenin and activating TGF-β/Smad signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Zhang,Chuanchuan Zheng,Wei Chen,Xiaoqiang Li,Jinshu Wang,Taikun Wang,Qi Zhao,Hao Huang,Yiting Li,Chengliang Yang,Kegong Xie,Shengcai Pan,Binghao Wang,Chong Wang,Yujin Tang,Kai Li,Jia Liu,Liqiang WangPubMed 37774483
- Clinical and translational medicine 13:e14152023Circular RNA circARPC1B functions as a stabilisation enhancer of Vimentin to prevent high cholesterol-induced articular cartilage degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiarui Li,Xiang Li,Shengji Zhou,Yuxin Wang,Tiantian Ying,Quan Wang,Yizheng Wu,Fengchao ZhaoPubMed 37740460
- Journal of cellular physiology 238:2668-26782023Loss of miR-204 and miR-211 shifts osteochondral balance and causes temporomandibular joint osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Huang,Yumei Lai,Jun Li,Lan ZhaoPubMed 37697972
- Advanced healthcare materials 12:e23013372023Nucleus Pulposus-Targeting Nanocarriers Facilitate Mirna-Based Therapeutics for Intervertebral Disc Degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhonghui Chen,Zhong Liao,Ming Liu,Fengfei Lin,Shunyou Chen,Geng Wang,Zhong Zheng,Boling Liu,Chaoxiong Li,Zheqiang Wang,Tianlai Chen,Hongzhe Huang,Qi Liao,Weiliang CuiPubMed 37625164
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/250 | Notes ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. |
Species Rat | Dilution info 1/250 | Notes ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. |
Species Human | Dilution info 1/250 | Notes ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. |
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Target data
Function
Metalloproteinase that plays an important role in connective tissue organization, development, inflammation and cell migration. Extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including ACAN, VCAN, BCAN and NCAN (PubMed:16133547, PubMed:18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its VCAN remodeling properties. Cleaves VCAN in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration (By similarity). Participates in development of brown adipose tissue and browning of white adipose tissue (By similarity). Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.
Alternative names
ADAMTS11, ADMP2, ADAMTS5, A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAM-TS 5, ADAM-TS5, ADAMTS-5, A disintegrin and metalloproteinase with thrombospondin motifs 11, ADMP-2, Aggrecanase-2, ADAM-TS 11, ADAMTS-11
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Anti-ADAMTS5 antibody (ab41037) is a rabbit polyclonal antibody detecting ADAMTS5 in Western Blot. Suitable for Human, Mouse, Rat.
- Over 190 publications
- Trusted since 2007
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-ADAMTS5 antibody (ab41037) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of ADAMTS5?
Anti-ADAMTS5 (ab41037) specifically detects a band for ADAMTS5 (UniProt: Q9UNA0) at a molecular weight of 102kDa.
Trusted by the scientific community
Anti-ADAMTS5 (ab41037) was first used in a scientific publication in 2007 and has been cited over 190 times in peer-reviewed journals.
Reviewed by scientists
Anti-ADAMTS5 (ab41037) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAMTS5 also known as aggrecanase-2 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Its approximate mass is 120 kDa. This enzyme functions primarily as a metalloprotease cleaving proteoglycans such as aggrecan thereby playing a significant role in the degradation of extracellular matrix components. ADAMTS5 is widely expressed in tissues such as cartilage. It is integral for maintaining tissue homeostasis and flexibility within the joint structures.
- Biological function summary
ADAMTS5 significantly contributes to the dynamic remodeling of the extracellular matrix. It operates without forming complexes with other proteins and functions independently. By cleaving aggrecan it regulates cartilage integrity balancing synthesis and breakdown of matrix constituents. This balance is essential for facilitating normal cartilage function and enables joints to resist load and stress.
- Pathways
Current understanding highlights that ADAMTS5 is critical within pathways like cartilage catabolism and inflammation response. ADAMTS5 activity initiates extracellular matrix remodeling by interacting with proteins such as aggrecan and collagen II. It plays a role in precise regulatory processes that manage cartilage homeostasis and maintains stability in structural integrity through its catabolic actions.
- Associated diseases and disorders
ADAMTS5 has strong links to osteoarthritis and rheumatoid arthritis. Pathological overexpression or dysregulated activity of ADAMTS5 can accelerate cartilage degradation within these conditions. ADAMTS5 interacts with proteins involved in inflammatory pathways and its activity correlates with inflammatory cytokines. Understanding its role is important for designing therapies aimed at inhibiting its excessive action and developing strategies to slow down the disease progression in arthritis patients.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-ADAMTS5 antibody (ab41037)
ab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.
All lanes: Western blot - Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate at 10 µg
Lane 4: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 5: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 6: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 7: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 8: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 9: Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Lane 10: Liver (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 73 kDa
Western blot - Anti-ADAMTS5 antibody (ab41037)
ab41037 appears to detect a 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. The doublet observed in human uterus lysate is possibly due to glycosylation.
All lanes: Western blot - Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution
Lane 1: Human placenta tissue lysate - total protein (ab29745) at 10 µg
Lane 2: Western blot - Mouse placenta normal tissue lysate - total protein (Mouse placenta normal tissue lysate - total protein ab29139) at 10 µg
Lane 3: Uterus (Human) Whole Cell Lysate - normal tissue at 10 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 27 kDa, 73 kDa
Western blot - Anti-ADAMTS5 antibody (ab41037)
ab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.
All lanes: Western blot - Anti-ADAMTS5 antibody (ab41037) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 55 kDa, 73 kDa
Exposure time: 12min
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