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AB32565

Anti-ADAMTS9 antibody

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(3 Publications)

Rabbit Polyclonal ADAMTS9 antibody. Suitable for ICC and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ADAMTS9 aa 650-700.

View Alternative Names

KIAA1312, ADAMTS9, A disintegrin and metalloproteinase with thrombospondin motifs 9, ADAM-TS 9, ADAM-TS9, ADAMTS-9

1 Images
Immunocytochemistry - Anti-ADAMTS9 antibody (AB32565)
  • ICC

Supplier Data

Immunocytochemistry - Anti-ADAMTS9 antibody (AB32565)

Immunofluorescence analysis of ADAMTS9 in SH-SY5Y cells using ab32565. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab32565 at 2 μg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2000 dilution for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. (Panel d) represents the merged image showing localization in extracellular matrix. (Panel e) shows the no primary antibody control. The images were captured at 60X magnification.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC

applications

Immunogen

Synthetic Peptide within Human ADAMTS9 aa 650-700. The exact immunogen used to generate this antibody is proprietary information.

Q9P2N4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "<p></p>" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Xenopus laevis": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody is Antigen affinity chromatography purified.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ADAMTS9 protein also known as A Disintegrin and Metalloproteinase with Thrombospondin motifs 9 plays a mechanical role as a metalloproteinase enzyme. It features a molecular weight of approximately 193 kDa. ADAMTS9 is expressed in various tissues including the heart pancreas and kidney among others. This protein undergoes post-translational modifications that impact its mechanical functions offering unique regulation mechanisms for tissue remodeling and extracellular matrix degradation.
Biological function summary

ADAMTS9 influences processes associated with maintaining extracellular matrix integrity and remodeling. It participates in the cleavage of proteoglycans hence modulating cellular environments. This protein does not typically form large complexes but it interacts with substrates that are part of the complex networks found in connective tissues. Its enzymatic activity is subject to intervention by specific inhibitors which can significantly affect tissue homeostasis.

Pathways

ADAMTS9 is involved in the TGF-beta signaling and inflammatory response pathways. The TGF-beta signaling pathway is integral to regulating cellular growth and differentiation. In the inflammatory response ADAMTS9 interacts with proteins like aggrecan playing a part in inflammation-related tissue devastation. These pathways demonstrate the multifunctional nature of ADAMTS9 in cellular and physiological contexts.

ADAMTS9 is notably associated with arthritis and cancer progression. In arthritis the enzymatic activity leads to tissue degradation by breaking down cartilage components linking it to disease progression. In tumors its altered expression may influence cancer cell invasion and metastasis. In these conditions ADAMTS9 interacts with proteins such as versican contributing to pathological changes in tissue architecture and cellular behavior.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaves the large aggregating proteoglycans, aggrecan (at the '1838-Glu-|-Ala-1839' site) and versican (at the '1428-Glu-|-Ala-1429' site). Has a protease-independent function in promoting the transport from the endoplasmic reticulum to the Golgi apparatus of a variety of secretory cargos.
See full target information ADAMTS9
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Life sciences 260:118237 PubMed32781068

2020

Upregulation of TNF-α and IL-6 induces preterm premature rupture of membranes by activation of ADAMTS-9 in embryonic membrane cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Li,Xiaomin Zhao,Shanshan Li,Xu Chen,Hongyan Cui,Ying Chang,Rongxin Zhang

Bioscience reports 40: PubMed32744323

2020

Lnc RNA ZFAS1 regulates the proliferation, apoptosis, inflammatory response and autophagy of fibroblast-like synoviocytes via miR-2682-5p/ADAMTS9 axis in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Yang,Wei Yin,Yan Ding,Fan Liu

Journal of the American Heart Association 7:e010427 PubMed30371322

2018

Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Sayantan Jana,Hao Zhang,Gary D Lopaschuk,Darren H Freed,Consolato Sergi,Paul F Kantor,Gavin Y Oudit,Zamaneh Kassiri
View all publications
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