AB327100

Anti-ADAR Antibody [CL0176]

Name: Anti-ADAR Antibody [CL0176] (ab327100) | Abcam
Brand: Abcam Limited
SKU: AB327100
Price: 580 USD
Availability: InStock

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Mouse Monoclonal ADAR1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ADAR.

View Alternative Names

ADAR1, DSRAD, G1P1, IFI4, ADAR, Double-stranded RNA-specific adenosine deaminase, DRADA, 136 kDa double-stranded RNA-binding protein, Interferon-inducible protein 4, K88DSRBP, p136, IFI-4

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-ADAR Antibody [CL0176] (AB327100)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADAR Antibody [CL0176] (AB327100)

Immunofluorescence staining of A549 cells using ab327100 at 2–10 µg/ml, showing specific staining in the nucleoplasm and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR Antibody [CL0176] (AB327100)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR Antibody [CL0176] (AB327100)

Immunohistochemical staining of human testis using ab327100 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in cells in seminiferous ducts.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR Antibody [CL0176] (AB327100)

Immunohistochemical staining of human cerebral cortex using ab327100 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in neurons.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR Antibody [CL0176] (AB327100)

Immunohistochemical staining of human kidney using ab327100 at 1 : 500–1 : 1000 dilution, showing strong nuclear positivity in cells in tubules.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR Antibody [CL0176] (AB327100)

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using ab327100 at 1 : 500–1 : 1000 dilution. Corresponding ADAR RNA-seq data are presented for the same tissues.

Supplier Data

Western blot - Anti-ADAR Antibody [CL0176] (AB327100)

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using ab327100 at 1 µg/ml. Remaining relative intensity is presented. Loading control : Anti-GAPDH.

All lanes:

Western blot - Anti-ADAR Antibody [CL0176] (ab327100)

Lane 2:

U-251MG cells transfected with control siRNA

Lane 3:

U-251MG cells transfected with target specific siRNA probe #1

Lane 4:

U-251MG cells transfected with target specific siRNA probe #2

Predicted band size: 136.07 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR Antibody [CL0176] (AB327100)

Immunohistochemical staining of human skeletal muscle using ab327100 at 1 : 500–1 : 1000 dilution, showing no positivity in myocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL0176

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ADAR.

P55265

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing (PubMed : 12618436, PubMed : 7565688, PubMed : 7972084). This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins since the translational machinery read the inosine as a guanosine; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include : bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include : hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence. Can enhance viral replication of HDV via A-to-I editing at a site designated as amber/W, thereby changing an UAG amber stop codon to an UIG tryptophan (W) codon that permits synthesis of the large delta antigen (L-HDAg) which has a key role in the assembly of viral particles. However, high levels of ADAR1 inhibit HDV replication.

See full target information ADAR

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Product promise

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