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Anti-ADAR1 antibody [EPR7033] (ab126745) is a rabbit monoclonal antibody that is used to detect ADAR1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human samples.



- Specificity confirmed with ADAR1 knockout cell line validation

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Images

Western blot - Anti-ADAR1 antibody [EPR7033] (AB126745), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR1 antibody [EPR7033] (AB126745), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ADAR1 antibody [EPR7033] (AB126745), expandable thumbnail
  • Western blot - Anti-ADAR1 antibody [EPR7033] (AB126745), expandable thumbnail
  • Western blot - Anti-ADAR1 antibody [EPR7033] (AB126745), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing (PubMed:12618436, PubMed:7565688, PubMed:7972084). This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins since the translational machinery read the inosine as a guanosine; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence. Can enhance viral replication of HDV via A-to-I editing at a site designated as amber/W, thereby changing an UAG amber stop codon to an UIG tryptophan (W) codon that permits synthesis of the large delta antigen (L-HDAg) which has a key role in the assembly of viral particles. However, high levels of ADAR1 inhibit HDV replication.

Alternative names

ADAR1, DSRAD, G1P1, IFI4, ADAR, Double-stranded RNA-specific adenosine deaminase, DRADA, 136 kDa double-stranded RNA-binding protein, Interferon-inducible protein 4, K88DSRBP, p136, IFI-4

Recommended products

Anti-ADAR1 antibody [EPR7033] (ab126745) is a rabbit monoclonal antibody that is used to detect ADAR1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human samples.



- Specificity confirmed with ADAR1 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7033
Purification technique
Affinity purification Protein A
Specificity

The immunogen is designed to detect the p150 isoform and not the p110.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-ADAR1 antibody [EPR7033] (ab126745) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), in Human samples.

What is the molecular weight of ADAR1?


Anti-ADAR1 [EPR7033] (ab126745) specifically detects a band for ADAR1 (UniProt: P55265) at a molecular weight of 136kDa.

Recommended positive controls


WB: HEK293T, HeLa, Ramos and SH-SY5Y cell lysates.IHC-P: Human brain tissueFlow Cyt (intra): HeLa cells

Trusted by the scientific community


Anti-ADAR1 [EPR7033] (ab126745) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed


The specificity of Anti-ADAR1 antibody [EPR7033] (ab126745) has been confirmed by Western blot testing in ADAR Knockout HAP1 cells.



Other related products


We have a range of other formats of antibody clone [EPR7033] also available for your convenience:
ab126745, HRP - HRP Anti-ADAR1 antibody [EPR7033] ab206086, Carrier free - Anti-ADAR1 antibody [EPR7033] - BSA and Azide free ab240029, APC - APC Anti-ADAR1 antibody [EPR7033] ab310818, PE - PE Anti-ADAR1 antibody [EPR7033] ab310896, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-ADAR1 antibody [EPR7033] ab310981, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-ADAR1 antibody [EPR7033] ab311103, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-ADAR1 antibody [EPR7033] ab311722, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-ADAR1 antibody [EPR7033] ab312998, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-ADAR1 antibody [EPR7033] ab313203, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-ADAR1 antibody [EPR7033] ab321014



Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ADAR1 also known as RNA-specific adenosine deaminase 1 or ADAR is an enzyme with a mass of approximately 150 kDa. This protein targets double-stranded RNA (dsRNA) and acts mechanistically to convert adenosine to inosine in pre-mRNA sequences a process known as A-to-I RNA editing. ADAR1 is expressed in numerous tissues with high levels in the brain liver and lungs. Its localization within cells can vary often found in both the nucleus and cytoplasm which influences its function.

Biological function summary

ADAR1 plays a role in the regulation of RNA molecules affecting their stability and translation. It is linked to the editosome complex working alongside other proteins to perform RNA editing tasks. By modifying the coding potential of mRNAs ADAR1 contributes to the diversity of proteomes and helps manage the responses to viral RNAs giving the immune system tools to recognize endogenous and exogenous RNA.

Pathways

ADAR1 is significant in the interferon signaling pathway and RNA processing pathways. It operates in coordination with proteins like PKR which is involved in the response to viral infections. ADAR1 ensures that the immune response is not directed against the self highlighting its role in the regulation of the innate immune system. These pathways are critical in maintaining homeostasis and preventing unchecked immune responses.

Associated diseases and disorders

Mutations or dysregulation of ADAR1 have associations with autoimmune diseases like Aicardi-Goutieres syndrome and certain cancers. The enzyme's role in editing RNA makes it essential in preventing inappropriate immune attacks against the body's own cells highlighting its interaction with MDA5 another protein involved in immune regulation. Understanding ADAR1 and its related pathways may offer potential therapeutic targets for these conditions including exploration into ADAR1 inhibitors as interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-ADAR1 antibody [EPR7033] (ab126745), expandable thumbnail

    Western blot - Anti-ADAR1 antibody [EPR7033] (ab126745)

    Lanes 1-3: Merged signal (red and green). Green - ab126745 observed at 130 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab126745 Anti-ADAR1 antibody [EPR7033] was shown to specifically react with ADAR1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human ADAR (ADAR1) knockout HEK-293T cell line ab266846 (knockout cell lysate Human ADAR (ADAR1) knockout HEK-293T cell lysate ab257131) was used. Wild-type and ADAR1 knockout samples were subjected to SDS-PAGE. ab126745 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ADAR1 antibody [EPR7033] (ab126745) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: ADAR knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human ADAR (ADAR1) knockout HEK-293T cell line (Human ADAR (ADAR1) knockout HEK-293T cell line ab266846)

    Lane 3: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 136 kDa, 19 kDa, 29 kDa, 35 kDa, 36 kDa

    Observed band size: 130 kDa, 20 kDa, 29 kDa, 35 kDa, 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR1 antibody [EPR7033] (ab126745), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAR1 antibody [EPR7033] (ab126745)

    ab126745, at 1/50 dilution, staining ADAR1 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-ADAR1 antibody [EPR7033] (ab126745), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ADAR1 antibody [EPR7033] (ab126745)

    Intracellular flow cytometric analysis of permeabilized Ramos cells, staining ADAR1 (red) with ab126745. 1x106 cells were collected and washed with blocking buffer. Cells were fixed with 2% paraformaldehyde, permeabilized with 1X FACS permeabilizing solution and blocked with blocking buffer for 30 minutes at room temperature. Cells were incubated with primary antibody (1/10) for 30 minutes at room temperature before a Fluorescently-conjugated secondary antibody or 30 min at room temperature. A rabbit IgG was used as a negative control (green).

  • Western blot - Anti-ADAR1 antibody [EPR7033] (ab126745), expandable thumbnail

    Western blot - Anti-ADAR1 antibody [EPR7033] (ab126745)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: ADAR1 knockout HAP1 cell lysate (20 μg)
    Lane 3: HepG2 cell lysate (20 μg)
    Lane 4: HeLa cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab126745 observed at 150 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab126745 was shown to recognize ADAR1 when ADAR1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ADAR1 knockout samples were subjected to SDS-PAGE. ab126745 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-ADAR1 antibody [EPR7033] (ab126745)

    Predicted band size: 136 kDa

    Observed band size: 150 kDa

  • Western blot - Anti-ADAR1 antibody [EPR7033] (ab126745), expandable thumbnail

    Western blot - Anti-ADAR1 antibody [EPR7033] (ab126745)

    All lanes: Western blot - Anti-ADAR1 antibody [EPR7033] (ab126745) at 1/1000 dilution

    Lane 1: HeLa (treated with IFN-alpha) cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: Ramos cell lysate at 10 µg

    Lane 4: SH-SY5Y cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 136 kDa

    Observed band size: 150 kDa

  • Immunoprecipitation - Anti-ADAR1 antibody [EPR7033] (ab126745), expandable thumbnail

    Immunoprecipitation - Anti-ADAR1 antibody [EPR7033] (ab126745)

    ADAR1 was immunoprecipitated from 0.35 mg HeLa whole cell lysate with Anti-ADAR1 antibody [EPR25431-60] ab307585 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-ADAR1 antibody [EPR25431-60] ab307585 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 2: Anti-ADAR1 antibody [EPR25431-60] ab307585 IP in HeLa whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ADAR1 antibody [EPR25431-60] ab307585 in HeLa whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: Lanes 1-3: 5 seconds (left), Lanes 1-3: 3 seconds (right).
    The IP experiment was performed by Anti-ADAR1 antibody [EPR25431-60] ab307585 using HeLa cells. On the left the IP blot was probed with Anti-ADAR1 antibody [EPR25431-60] ab307585 and on the right the blot was probed by another anti-ADAR1 antibody (ab126745)(1:1000 dilution).

    Lane 2: Immunoprecipitation - Anti-ADAR1 antibody [EPR25431-60] (Anti-ADAR1 antibody [EPR25431-60] ab307585) at 1/30 dilution

    Lane 2: Immunoprecipitation - Anti-ADAR1 antibody [EPR7033] (ab126745) at 1/1000 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3s

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Product protocols

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