Rabbit Polyclonal ADAT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ADAT1 aa 1-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ADAT1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BUB4
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Specifically deaminates adenosine-37 to inosine in tRNA-Ala.
Alternative names
tRNA-specific adenosine deaminase 1, hADAT1, tRNA-specific adenosine-37 deaminase, ADAT1
Recommended products
Rabbit Polyclonal ADAT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ADAT1 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ADAT1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BUB4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ADAT1 also known as ADAT one or Adenosine Deaminase Acting on tRNA 1 is an enzyme that catalyzes the deamination of adenosine to inosine in transfer RNAs. This enzyme is critical for the proper modification of tRNA ensuring accurate protein synthesis. ADAT1 is composed of approximately 55 kDa polypeptides and is expressed in various tissues with notably higher levels in the testes and brain. It modifies tRNA anticodons which are necessary for precise translation of the genetic code.
- Biological function summary
The function of tRNA modification by ADAT1 impacts protein translation accuracy and efficiency. It is a part of a larger complex involving other tRNA-modifying enzymes. Proper functioning of ADAT1 is necessary to maintain the integrity of protein synthesis as it impacts the decoding of genetic information during translation by tRNA. This process helps cells to correctly read the mRNA sequences and modify the protein expression accordingly.
- Pathways
TRNA modification by ADAT1 plays an important role in the protein synthesis pathway. This modification enhances the fidelity and efficiency of translating genetic information from mRNA into functional proteins. The enzyme works closely with other tRNA modification enzymes such as ADAT2 and ADAT3 which are involved in post-transcriptional modification of tRNA. These interactions ensure that tRNAs have the correct structure and function to support normal cell physiology.
- Associated diseases and disorders
Malfunction of ADAT1 is associated with neurological disorders and some types of cancer. This is due to the enzyme's role in maintaining protein synthesis fidelity which can affect cellular health. Abnormal activity or expression of ADAT1 could disrupt protein balance in cells linking it to disorders like intellectual disability and certain cancers. The reduced function of ADAT1 affects its interaction with other proteins like ADAR which are involved in RNA editing and are important for normal cellular processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-ADAT1 antibody (ab122662)
Immunocytochemistry/immunofluorescence analysis of human cell line SiHa labelling ADAT1 with ab122662 at 2 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT1 antibody (ab122662)
ab122662 at 1/50 dilution staining ADAT1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Western blot - Anti-ADAT1 antibody (ab122662)
All lanes: Western blot - Anti-ADAT1 antibody (ab122662) at 1/250 dilution
Lane 1: RT-4 cell lysate
Lane 2: U-251 MG cell lysate
Lane 3: Human plasma lysate
Lane 4: Human liver tissue lysate
Lane 5: Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 55 kDa
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