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AB122662

Anti-ADAT1 antibody

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(1 Publication)

Rabbit Polyclonal ADAT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADAT1 aa 1-100.

View Alternative Names

tRNA-specific adenosine deaminase 1, hADAT1, tRNA-specific adenosine-37 deaminase, ADAT1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT1 antibody (AB122662)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT1 antibody (AB122662)

ab122662 at 1/50 dilution staining ADAT1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-ADAT1 antibody (AB122662)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ADAT1 antibody (AB122662)

Immunocytochemistry/immunofluorescence analysis of human cell line SiHa labelling ADAT1 with ab122662 at 2 μg/mL.

Western blot - Anti-ADAT1 antibody (AB122662)
  • WB

Unknown

Western blot - Anti-ADAT1 antibody (AB122662)

All lanes:

Western blot - Anti-ADAT1 antibody (ab122662) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver tissue lysate

Lane 5:

Human tonsil tissue lysate

Predicted band size: 55 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ADAT1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9BUB4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAT1 also known as ADAT one or Adenosine Deaminase Acting on tRNA 1 is an enzyme that catalyzes the deamination of adenosine to inosine in transfer RNAs. This enzyme is critical for the proper modification of tRNA ensuring accurate protein synthesis. ADAT1 is composed of approximately 55 kDa polypeptides and is expressed in various tissues with notably higher levels in the testes and brain. It modifies tRNA anticodons which are necessary for precise translation of the genetic code.
Biological function summary

The function of tRNA modification by ADAT1 impacts protein translation accuracy and efficiency. It is a part of a larger complex involving other tRNA-modifying enzymes. Proper functioning of ADAT1 is necessary to maintain the integrity of protein synthesis as it impacts the decoding of genetic information during translation by tRNA. This process helps cells to correctly read the mRNA sequences and modify the protein expression accordingly.

Pathways

TRNA modification by ADAT1 plays an important role in the protein synthesis pathway. This modification enhances the fidelity and efficiency of translating genetic information from mRNA into functional proteins. The enzyme works closely with other tRNA modification enzymes such as ADAT2 and ADAT3 which are involved in post-transcriptional modification of tRNA. These interactions ensure that tRNAs have the correct structure and function to support normal cell physiology.

Malfunction of ADAT1 is associated with neurological disorders and some types of cancer. This is due to the enzyme's role in maintaining protein synthesis fidelity which can affect cellular health. Abnormal activity or expression of ADAT1 could disrupt protein balance in cells linking it to disorders like intellectual disability and certain cancers. The reduced function of ADAT1 affects its interaction with other proteins like ADAR which are involved in RNA editing and are important for normal cellular processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Specifically deaminates adenosine-37 to inosine in tRNA-Ala.
See full target information ADAT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 44:1820-1830 PubMed32379372

2020

TMEM98 mRNA promotes proliferation and invasion of gastric cells by directly interacting with NF90 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Xudong Ao,Xinxin Li,Yongxia Chen,Zhichao Zang,Weichun Guo,Junqing Liang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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