JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235341

Anti-ADAT1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal ADAT1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADAT1 aa 1 to C-terminus.

View Alternative Names

tRNA-specific adenosine deaminase 1, hADAT1, tRNA-specific adenosine-37 deaminase, ADAT1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT1 antibody (AB235341)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT1 antibody (AB235341)

Paraffin-embedded human skin tissue stained for ADAT1 with ab235341 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT1 antibody (AB235341)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT1 antibody (AB235341)

Paraffin-embedded human tonsil tissue stained for ADAT1 with ab235341 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ADAT1 antibody (AB235341)
  • WB

Supplier Data

Western blot - Anti-ADAT1 antibody (AB235341)

All lanes:

Western blot - Anti-ADAT1 antibody (ab235341) at 1/500 dilution

Lane 1:

Mouse kidney tissue lysate

Lane 2:

THP-1 (Human monocytic leukemia cell line) whole cell lysate

Lane 3:

Mouse liver tissue lysate

Lane 4:

MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate

Lane 5:

HL-60 (Human promyelocytic leukemia cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ADAT1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BUB4

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAT1 also known as ADAT one or Adenosine Deaminase Acting on tRNA 1 is an enzyme that catalyzes the deamination of adenosine to inosine in transfer RNAs. This enzyme is critical for the proper modification of tRNA ensuring accurate protein synthesis. ADAT1 is composed of approximately 55 kDa polypeptides and is expressed in various tissues with notably higher levels in the testes and brain. It modifies tRNA anticodons which are necessary for precise translation of the genetic code.
Biological function summary

The function of tRNA modification by ADAT1 impacts protein translation accuracy and efficiency. It is a part of a larger complex involving other tRNA-modifying enzymes. Proper functioning of ADAT1 is necessary to maintain the integrity of protein synthesis as it impacts the decoding of genetic information during translation by tRNA. This process helps cells to correctly read the mRNA sequences and modify the protein expression accordingly.

Pathways

TRNA modification by ADAT1 plays an important role in the protein synthesis pathway. This modification enhances the fidelity and efficiency of translating genetic information from mRNA into functional proteins. The enzyme works closely with other tRNA modification enzymes such as ADAT2 and ADAT3 which are involved in post-transcriptional modification of tRNA. These interactions ensure that tRNAs have the correct structure and function to support normal cell physiology.

Malfunction of ADAT1 is associated with neurological disorders and some types of cancer. This is due to the enzyme's role in maintaining protein synthesis fidelity which can affect cellular health. Abnormal activity or expression of ADAT1 could disrupt protein balance in cells linking it to disorders like intellectual disability and certain cancers. The reduced function of ADAT1 affects its interaction with other proteins like ADAR which are involved in RNA editing and are important for normal cellular processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Specifically deaminates adenosine-37 to inosine in tRNA-Ala.
See full target information ADAT1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 40:e106336 PubMed33595114

2021

NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.

Applications

Unspecified application

Species

Unspecified reactive species

Maya Raghunandan,Dan Geelen,Eva Majerova,Anabelle Decottignies
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB122662

Anti-ADAT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT1 antibody (AB122662)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB176689

Anti-ZNF384 antibody

Immunoprecipitation - Anti-ZNF384 antibody (AB176689)

  • 5
  • 1
Primary Antibodies

AB237050

Anti-CBWD2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CBWD2 antibody (AB237050)

  • 0
  • 0
Primary Antibodies

AB119779

Anti-Tristetraprolin/TTP antibody [OTI1A2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI1A2] (AB119779)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com