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AB247133

Anti-ADAT3 antibody

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Rabbit Polyclonal ADAT3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ADAT3 aa 250-350.

View Alternative Names

TAD3, ADAT3, tRNA-specific adenosine-34 deaminase regulatory subunit ADAT3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT3 antibody (AB247133)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT3 antibody (AB247133)

Paraffin-embedded human stomach tissue stained for ADAT3 using ab247133 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ADAT3 antibody (AB247133)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ADAT3 antibody (AB247133)

PFA fixed, Triton X-100 permeabilized HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling ADAT3 using ab247133 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT3 antibody (AB247133)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT3 antibody (AB247133)

Paraffin-embedded human cerebral cortex tissue stained for ADAT3 using ab247133 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT3 antibody (AB247133)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT3 antibody (AB247133)

Paraffin-embedded human testis tissue stained for ADAT3 using ab247133 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT3 antibody (AB247133)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT3 antibody (AB247133)

Paraffin-embedded human duodenum tissue stained for ADAT3 using ab247133 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ADAT3 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q96EY9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADAT3 also known as ADAR3 or Adenosine Deaminase Acting on tRNA 3 is an enzyme with a molecular mass of approximately 30 kDa. It belongs to the family of ADAT enzymes which perform adenosine-to-inosine editing in RNA molecules. This protein expresses mainly in the brain and various other tissues indicating its availability across the body. The presence of ADAT3 is essential in regions where RNA editing plays a role in transcriptome diversity.
Biological function summary

The function of adenosine deamination significantly impacts RNA molecules. ADAT3 participates actively in the tRNA editing process. It becomes part of the ADAT complex which also includes ADAT2. This complex catalyzes the deamination of adenosine to inosine at the wobble position of certain tRNAs altering the genetic code potential during translation which contributes to the optimization of protein synthesis.

Pathways

ADAT3 has essential roles in tRNA modification and processing pathways. It impacts the expression and functionality of various genes by adjusting tRNA decoding accuracy. Through its role in RNA editing it relates closely to other RNA-editing ADAR proteins which share some mechanistic similarities. These proteins collaborate influencing broader pathways involving RNA metabolism and gene expression regulation.

Mutations or malfunctions in ADAT3 associate with intellectual disabilities specifically in conditions like neurodevelopmental disorders. The inability to perform adequate RNA editing can lead to defects in neural communication and development. Additionally ADAT3 is connected to specific proteins linked to cognitive function impairments indicating its significance in maintaining normal neurological function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic subunit of the tRNA-specific adenosine-34 deaminase complex, composed of the ADAT2 catalytic subunit and the ADAT3 regulatory subunit, which deaminates adenosine-34 (the first, also called wobble position of the anticodon) to inosine in many tRNAs. Inosine-34 allows the decoding of 3 different nucleotides at the third position of mRNA codons, as inosine is able to pair with U, C, and A. Required for binding of the ADAT2-ADAT3 complex to tRNA through its N-terminus, which rotates with respect to the catalytic domain of the complex, formed by ADAT2 and the ADAT3 C-terminal domain, to position the tRNA anticodon stem-loop correctly in the ADAT2 active site. The ADAT2-ADAT3 complex is required for radial migration of projection neurons in the developing brain cortex, and the catalytic activity of the complex is necessary for this function.
See full target information ADAT3
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Product promise

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