JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB230897

Anti-ADCK3 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal ADCK3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human COQ8A conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ADCK3, CABC1, PP265, COQ8A, Chaperone activity of bc1 complex-like, Coenzyme Q protein 8A, aarF domain-containing protein kinase 3, Chaperone-ABC1-like

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ADCK3 antibody (AB230897)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADCK3 antibody (AB230897)

Formalin-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ADCK3 (red) using ab230897 at 1/100 dilution followed by DyLight 594-congugated secondary antibody in ICC/IF.

The nuclear counter stain is DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCK3 antibody (AB230897)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCK3 antibody (AB230897)

Formalin-fixed, paraffin-embedded human thymus tissue stained for ADCK3 using ab230897 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ADCK3 antibody (AB230897)
  • WB

Supplier Data

Western blot - Anti-ADCK3 antibody (AB230897)

All lanes:

Western blot - Anti-ADCK3 antibody (ab230897) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) whole cell lysate

Predicted band size: 72 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human COQ8A conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8NI60

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADCK3 also known as AarF domain-containing kinase 3 or COQ8A is a protein kinase with an approximate mass of 68 kDa. It is largely expressed in the mitochondria which indicates its role in cellular energy processes. The human ADCK3 gene encodes this protein and it serves a critical function in the biosynthesis of coenzyme Q10 an essential component of the mitochondrial electron transport chain. ADCK3's structure includes a kinase domain hinting at its enzymatic activity in phosphorylation processes.
Biological function summary

ADCK3 participates in the regulation of coenzyme Q10 levels an essential lipid-soluble antioxidant. The protein is a part of the mitochondrial electron transport chain complex contributing to the production of ATP. As a kinase ADCK3 influences the modulation of the complex's components through phosphorylation stabilizing the function and efficiency of energy conversion processes within mitochondria. Its role is critical for maintaining cellular energy balance and reducing oxidative stress.

Pathways

ADCK3 engages in the coenzyme Q10 biosynthesis pathway. It closely interacts with COQ proteins like COQ9 orchestrating the synthesis and function of the electron transport chain. Additionally it takes part in mitochondrial kinase pathways that ensure proper mitochondrial function. The cooperation between ADCK3 and other proteins such as COQ6 emphasizes its importance in maintaining mitochondrial integrity and energy metabolism.

Mutations in the ADCK3 gene have been linked to Coenzyme Q10 deficiency and spinocerebellar ataxia. Individuals with mutations often face progressive cerebellar atrophy a condition that affects motor skills and movement coordination. The association of ADCK3 with COQ6 in these disorders highlights its influence in the pathological development and underlines the potential therapeutic interest in modulating its activity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration (PubMed : 21296186, PubMed : 25498144, PubMed : 25540914, PubMed : 27499294). Its substrate specificity is unclear : does not show any protein kinase activity (PubMed : 25498144, PubMed : 27499294). Probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway, as suggested by its ability to bind coenzyme Q lipid intermediates (PubMed : 25498144, PubMed : 27499294). Shows an unusual selectivity for binding ADP over ATP (PubMed : 25498144).
See full target information COQ8A
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature immunology 26:279-293 PubMed39779871

2025

A multi-kinase inhibitor screen identifies inhibitors preserving stem-cell-like chimeric antigen receptor T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Song,Ourania Tsahouridis,Simone Stucchi,Tara Walhart,Sophie Mendell,P Brian Hardy,Matthew Axtman,Shiva K R Guduru,Thomas S K Gilbert,Lee M Graves,Laura E Herring,Barbara Savoldo,Xingcong Ma,Mark Woodcock,Justin J Milner,Anastasia Ivanova,Kenneth H Pearce,Yang Xu,Gianpietro Dotti

Cell death & disease 13:954 PubMed36371387

2022

ADCK1 is a potential therapeutic target of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-Biao Zhuo,Lun-Qing Zhu,Chen Yao,Xi-Hua Wang,Shi-Xian Li,Rong Wang,Yuan Li,Zhuo-Yan Ling

Frontiers in cardiovascular medicine 9:944521 PubMed36312243

2022

Integrated proteomic and metabolomic profile analyses of cardiac valves revealed molecular mechanisms and targets in calcific aortic valve disease.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Fu,Jing Wang,Lianqun Wang,Qiang Wang,Zhigang Guo,Meilin Xu,Nan Jiang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB40784

Anti-MAP3K4 antibody [EP551Y]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP3K4 antibody [EP551Y] (AB40784)

  • 0
  • 0
Primary Antibodies

AB109287

Anti-KAP1 antibody [EPR5216]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody [EPR5216] (AB109287)

  • 5
  • 4
Primary Antibodies

AB230881

Anti-RAB31 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB31 antibody (AB230881)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com