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AB223114

Anti-ADCK4 antibody

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(1 Publication)

Rabbit Polyclonal ADCK4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COQ8B aa 1-150.

View Alternative Names

ADCK4, COQ8B, AarF domain-containing protein kinase 4, Coenzyme Q protein 8B

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ADCK4 antibody (AB223114)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADCK4 antibody (AB223114)

A549 (human lung carcinoma cell line) cells stained for ADCK4 (green) using ab223114 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCK4 antibody (AB223114)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCK4 antibody (AB223114)

Paraffin-embedded human kidney tissue stained for ADCK4 using ab223114 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCK4 antibody (AB223114)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCK4 antibody (AB223114)

Paraffin-embedded human melanoma cancer tissue stained for ADCK4 using ab223114 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-ADCK4 antibody (AB223114)
  • WB

Supplier Data

Western blot - Anti-ADCK4 antibody (AB223114)

All lanes:

Western blot - Anti-ADCK4 antibody (ab223114) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 60 kDa

Observed band size: 56 kDa,60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human COQ8B aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q96D53

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADCK4 also known as aarF domain containing kinase 4 is a protein kinase with a molecular weight of approximately 49 kDa. It mainly exists in the mitochondria where it plays a significant role in cellular energy production. Expression of ADCK4 appears highest in the kidney and to a lesser extent in other tissues. As a kinase its mechanical function depends on phosphorylating various substrates which is necessary for the maintenance of mitochondrial function.
Biological function summary

ADCK4 participates in the biosynthesis of coenzyme Q10 a vital component of the electron transport chain. This protein forms part of a larger complex known as the coenzyme Q biosynthesis complex where it helps maintain cellular energy and metabolic balance. It interacts closely with other proteins involved in this pathway to regulate coenzyme Q10 levels which affects multiple cellular processes including ATP production.

Pathways

ADCK4 significantly integrates into the mitochondrial respiratory chain pathway and the broader metabolic energy pathway. It aids in the synthesis of coenzyme Q10 alongside proteins like COQ6 and COQ7 directly impacting the efficiency of energy production in mitochondria. This involvement supports metabolic activities that are critical for cellular homeostasis and energy regulation.

Mutations or dysfunctions in ADCK4 correlate with nephrotic syndrome and coenzyme Q10 deficiency. Individuals with nephrotic syndrome often show altered ADCK4 activity which affects coenzyme Q10 synthesis and leads to kidney-related complications. Additionally connections between ADCK4 and the action of COQ2 indicate potential pathways through which mitochondrial and renal disorders might develop from inadequate coenzyme Q10 levels.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration (PubMed : 24270420, PubMed : 36302899, PubMed : 38425362). Its substrate specificity is still unclear : may act as a protein kinase that mediates phosphorylation of COQ3 (PubMed : 38425362). According to other reports, acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway, as suggested by its ability to bind coenzyme Q lipid intermediates (By similarity). However, the small molecule kinase activity was not confirmed by another publication (PubMed : 38425362). Required for podocyte migration (PubMed : 24270420).
See full target information COQ8B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 9:4224-4238 PubMed35117790

2020

High expression of GNB4 predicts poor prognosis in patients with -positive advanced gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianpeng Gao,Teng Yu,Yi Xuan,Zhenglun Zhu
View all publications
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