JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB203204

Anti-ADCY10/SAC antibody

5

(1 Review)

|

(4 Publications)

Rabbit Polyclonal ADCY10/SAC antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADCY10 aa 1100-1200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

SAC, ADCY10, Adenylate cyclase type 10, AH-related protein, Adenylate cyclase homolog, Germ cell soluble adenylyl cyclase, Testicular soluble adenylyl cyclase, hsAC, sAC

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCY10/SAC antibody (AB203204)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCY10/SAC antibody (AB203204)

Immunohistochemistry on Formalin/PFA-fixed paraffin-embedded human liver cancer tissue labeling ADCY10/SAC with ab203204 at a 1/100 dilution, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCY10/SAC antibody (AB203204)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCY10/SAC antibody (AB203204)

Immunohistochemistry on Formalin/PFA-fixed paraffin-embedded rat brain tissue labeling ADCY10/SAC with ab203204 at a 1/200 dilution, followed by conjugation to the secondary antibody Goat anti-rabbit IgG-Cy3 at a 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCY10/SAC antibody (AB203204)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCY10/SAC antibody (AB203204)

Immunohistochemistry on Formalin/PFA-fixed paraffin-embedded rat lung tissue labeling ADCY10/SAC with ab203204 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ADCY10 aa 1100-1200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q96PN6

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ADCY10 protein also known as soluble adenylate cyclase (sAC) functions as an enzyme that produces cyclic adenosine monophosphate (cAMP) from ATP. Weighing approximately 187 kDa it acts in a unique way compared to other adenylate cyclases due to its solubility in the cytosol rather than being membrane-bound. ADCY10 expresses in various tissues with abundant expression observed in testis and sperm cells. Its expression pattern and physicochemical properties underpin its distinctiveness among the cyclase family.
Biological function summary

ADCY10 plays a significant role in generating cAMP in response to bicarbonate and calcium ions which are key to sperm motility and fertilization processes. This catalytic activity heavily influences metabolic activities and sperm functions cementing its role in reproductive biology. Although it does not typically form a multi-protein complex ADCY10's interaction at a molecular level allows it to regulate vital cellular activities important to maintaining proper cellular responses to extracellular signals.

Pathways

ADCY10 serves in the cAMP signaling pathway an essential cascade that transduces extracellular signals into cellular responses. This pathway's crosstalk with others such as the calcium signaling pathway involves proteins like protein kinase A (PKA) which ADCY10 indirectly activates. Through these pathways ADCY10 impacts various physiological processes signifying its pivotal role in cellular communication and response.

ADCY10 has been linked to male infertility primarily due to its role in sperm activation and motility. Abnormalities in ADCY10 function might contribute to impaired sperm motility. Additionally ADCY10 has associations with other sperm-specific proteins such as CatSper channels which are important for proper sperm function. Disruptions in these proteins' interactions could further elucidate ADCY10's involvement in reproductive health issues.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the formation of the signaling molecule cAMP (PubMed : 12609998, PubMed : 15659711, PubMed : 24567411, PubMed : 24616449, PubMed : 25040695). May function as sensor that mediates responses to changes in cellular bicarbonate and CO(2) levels (PubMed : 15659711, PubMed : 17591988). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (By similarity). Involved in ciliary beat regulation (PubMed : 17591988).
See full target information ADCY10
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

Aging 13:9748-9765 PubMed33744851

2021

Long non-coding RNA DPP10-AS1 exerts anti-tumor effects on colon cancer the upregulation of ADCY1 by regulating microRNA-127-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Liu,Hui Zhao,Qiang Song,Guangmeng Li,Shuying Lin,Sheng Xiong

Investigative ophthalmology & visual science 57:5083-5092 PubMed27679853

2016

Soluble Adenylyl Cyclase Is Required for Retinal Ganglion Cell and Photoreceptor Differentiation.

Applications

IHC-Fr

Species

Mouse

Peter X Shaw,Jiahua Fang,Alan Sang,Yan Wang,Michael S Kapiloff,Jeffrey L Goldberg

Proceedings. Biological sciences 283: PubMed27147099

2016

The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Brett Nixon,Amanda L Anderson,Nathan D Smith,Robby McLeod,Stephen D Johnston
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB76238

Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (AB76238)

  • 0
  • 0
Primary Antibodies

AB259323

Anti-Cannabinoid Receptor I antibody [EPR23934-20]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Cannabinoid Receptor I antibody [EPR23934-20] (AB259323)

  • 4
  • 1
Primary Antibodies

AB5176

Anti-Histone H3 (phospho S10) antibody

Western blot - Anti-Histone H3 (phospho S10) antibody (AB5176)

  • 5
  • 57
Primary Antibodies

AB176689

Anti-ZNF384 antibody

Immunoprecipitation - Anti-ZNF384 antibody (AB176689)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com