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AB196686

Anti-ADCY8/AC8 antibody

4

(1 Review)

|

(4 Publications)

Rabbit Polyclonal ADCY8/AC8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADCY8.

View Alternative Names

Adenylate cyclase type 8, ATP pyrophosphate-lyase 8, Adenylate cyclase type VIII, Adenylyl cyclase 8, Ca(2+)/calmodulin-activated adenylyl cyclase, AC8, ADCY8

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCY8/AC8 antibody (AB196686)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADCY8/AC8 antibody (AB196686)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADCY8/AC8 using ab196686 at 1/50 dilution. Right panel was preincubated with blocking peptide.

Immunocytochemistry/ Immunofluorescence - Anti-ADCY8/AC8 antibody (AB196686)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ADCY8/AC8 antibody (AB196686)

Immunofluorescence analysis of A549 cells labeling ADCY8/AC8 antibody with ab196686 at 1/100 dilution. Right panel was preincubated with blocking peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ADCY8. The exact immunogen used to generate this antibody is proprietary information.

P40145

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ADCY8 also known as AC8 is a member of the adenylate cyclase family of enzymes. This enzyme catalyzes the conversion of ATP to cyclic AMP (cAMP) an important cellular signaling molecule. The molecular weight of ADCY8 is approximately 125 kDa. ADCY8 is primarily expressed in the brain particularly in areas such as the hippocampus cortex and cerebellum. It is also found in other tissues albeit in lower levels. As a membrane-bound enzyme ADCY8 activity is regulated by calcium and calmodulin which distinguishes it from other adenylate cyclase isoforms.
Biological function summary

The production of cAMP by ADCY8 plays a critical role in signal transduction pathways that mediate many physiological processes. ADCY8 operates as part of a larger signaling complex involving G-protein coupled receptors (GPCRs) which allows for dynamic regulation and integration of signals. Through cAMP ADCY8 influences processes such as synaptic plasticity learning and memory formation. Its activity can modulate the intracellular levels of calcium further affecting cellular responses.

Pathways

ADCY8 is a significant component in the cAMP signaling pathway where it regulates many downstream effects including protein kinase A (PKA) activation and cAMP responsive element-binding protein (CREB) phosphorylation. In addition ADCY8 links to the calcium signaling pathway as its activity is modulated by intracellular calcium levels. Through these pathways ADCY8 interacts with various proteins including calmodulin and PKA which are essential for translating extracellular signals into specific cellular responses.

Aberrant ADCY8 function has been implicated in neurological conditions such as depression and schizophrenia. These associations highlight the enzyme’s role in neurotransmitter-related signaling and brain function. ADCY8’s interaction with CREB and other proteins involved in the cAMP signaling pathway highlights its significance in mood regulation and cognitive processes. Understanding these connections may offer insights into potential therapeutic targets for these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the formation of cAMP in response to calcium entry leadings to cAMP signaling activation that affect processes suche as synaptic plasticity and insulin secretion. Plays a role in many brain functions, such as learning, memory, drug addiction, and anxiety modulation through regulation of synaptic plasticity by modulating long-term memory and long-term potentiation (LTP) through CREB transcription factor activity modulation. Plays a central role in insulin secretion by controlling glucose homeostasis through glucagon-like peptide 1 and glucose signaling pathway and maintains insulin secretion through calcium-dependent PKA activation leading to vesicle pool replenishment. Also, allows PTGER3 to induce potentiation of PTGER4-mediated PLA2 secretion by switching from a negative to a positive regulation, during the IL1B induced-dedifferentiation of smooth muscle cells.
See full target information ADCY8
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 14:e70769 PubMed40095726

2025

Exploring Mechanisms and Biomarkers of Breast Cancer Invasion and Migration: An Explainable Gene-Pathway-Compounds Neural Network.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Qian,Dandan Sun,Yichen Ma,Ling Qiu,Jie Wu

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

Cell death & disease 12:220 PubMed33637706

2021

Sunitinib treatment promotes metastasis of drug-resistant renal cell carcinoma via TFE3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Luchao Li,Shuo Zhao,Zhengfang Liu,Nianzhao Zhang,Shuo Pang,Jikai Liu,Cheng Liu,Yidong Fan

eLife 9: PubMed33201801

2020

Spatially compartmentalized phase regulation of a Ca-cAMP-PKA oscillatory circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Brian Tenner,Michael Getz,Brian Ross,Donya Ohadi,Christopher H Bohrer,Eric Greenwald,Sohum Mehta,Jie Xiao,Padmini Rangamani,Jin Zhang
View all publications
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