Rabbit Polyclonal ADDB antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADD2 aa 50-200.
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- Nature communications 12:1172202120S proteasomes secreted by the malaria parasite promote its growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElya Dekel,Dana Yaffe,Irit Rosenhek-Goldian,Gili Ben-Nissan,Yifat Ofir-Birin,Mattia I Morandi,Tamar Ziv,Xavier Sisquella,Matthew A Pimentel,Thomas Nebl,Eugene Kapp,Yael Ohana Daniel,Paula Abou Karam,Daniel Alfandari,Ron Rotkopf,Shimrit Malihi,Tal Block Temin,Debakshi Mullick,Or-Yam Revach,Ariel Rudik,Nir S Gov,Ido Azuri,Ziv Porat,Giulia Bergamaschi,Raya Sorkin,Gijs J L Wuite,Ori Avinoam,Teresa G Carvalho,Sidney R Cohen,Michal Sharon,Neta Regev-RudzkiPubMed 33608523
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ADD2 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link P35612
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Orangutan | Dilution info - | Notes - |
Target data
Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to the erythrocyte membrane receptor SLC2A1/GLUT1 and may therefore provide a link between the spectrin cytoskeleton to the plasma membrane. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.
Alternative names
ADDB, ADD2, Beta-adducin, Erythrocyte adducin subunit beta
Recommended products
Rabbit Polyclonal ADDB antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADD2 aa 50-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ADD2 aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link P35612
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Adducin 2 also known as ADD2 is an important cytoskeletal protein involved in the regulation of the actin-spectrin lattice an important structural component of the cell membrane. This protein has a molecular mass of approximately 83 kDa and is mainly expressed in brain and erythrocytes. Together with alpha-adducin ADD2 forms heterodimers or heterotrimers playing a significant role in maintaining cell shape and stability by promoting the association of the spectrin-actin lattice with the cell membrane.
- Biological function summary
Adducin 2 influences the architecture of the cytoskeleton by binding to actin filaments and recruiting spectrin facilitating the assembly of a robust spectrin-actin network. Adducin 2 functions within a protein complex known as the spectrin-actin cytoskeletal assembly ensuring the mechanical integrity of the cell. Its function is critical in neurons where it has a role in influencing cellular shape signal transduction and stability of synaptic structures.
- Pathways
The actin-associated role of Adducin 2 integrates within the Rho GTPase signaling pathway and the ERK/MAPK signaling pathway. Through these pathways Adducin 2 interacts with proteins such as RhoA and ERK orchestrating cytoskeletal arrangements and cellular dynamics. These interactions enable cellular processes like signal transduction which impact cell movement and shape alterations therefore playing a part in cell migration and neural plasticity.
- Associated diseases and disorders
Adducin 2 relates to hypertension and neurodegenerative disorders. Abnormal expression or functioning of the Adducin 2 protein impairs the cell's structural integrity potentially leading to changes in blood pressure with ADD1 being another involved protein when considering its role in blood pressure regulation. The perturbation in Adducin 2’s function also links with synaptic dysfunctions contributing to neurodegenerative conditions with cross-interactions involving proteins like spectrin notable in the pathophysiology of certain neural disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adducin 2 antibody (ab251821)
Paraffin-embedded human cerebral cortex tissue stained for Adducin 2 using ab251821 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adducin 2 antibody (ab251821)
Paraffin-embedded human pancreas tissue stained for Adducin 2 using ab251821 at 1/200 dilution in immunohistochemical analysis. Low expression, as expected.
Western blot - Anti-Adducin 2 antibody (ab251821)
All lanes: Western blot - Anti-Adducin 2 antibody (ab251821) at 0.4 µg/mL
Lane 1: RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2: U-2521 MG (human brain glioma cell line) whole cell lysate
Lane 3: Human plasma (IgG/HSA depleted)
Lane 4: Human liver lysate
Lane 5: Human tonsil lysate
Predicted band size: 81 kDa
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