Mouse Monoclonal ADT1 antibody. Suitable for IP, IHC-P and reacts with Cow, Human samples. Cited in 5 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (AB109864), expandable thumbnail](https://content.abcam.com/products/images/adenine-nucleotide-translocase-immunocapture-antibody-5f51bb5ag7-ab109864--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34895.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (AB109864)")
![Immunoprecipitation - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (AB109864), expandable thumbnail](https://content.abcam.com/products/images/adenine-nucleotide-translocase-immunocapture-antibody-5f51bb5ag7-ab109864--immunoprecipitation-img161838.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (AB109864)")
Publications
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- Mucosal immunology 10:1553-15682017Bcl-x mediates RIPK3-dependent necrosis in M. tuberculosis-infected macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesX Zhao et. al.PubMed 28401933
- The Biochemical journal 437:301-112011Hexokinase II acts through UCP3 to suppress mitochondrial reactive oxygen species production and maintain aerobic respiration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan J Mailloux,Tyler Dumouchel,Céline Aguer,Rob deKemp,Rob Beanlands,Mary-Ellen HarperPubMed 21554247
- Journal of cell science 123:894-9022010Sirtuin-3 deacetylation of cyclophilin D induces dissociation of hexokinase II from the mitochondria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNataly Shulga et. al.PubMed 20159966
- Experimental neurology 218:333-462009The mitochondrial permeability transition pore in motor neurons: involvement in the pathobiology of ALS mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLee J Martin et. al.PubMed 19272377
- Biochimica et biophysica acta 1659:206-112004Focused proteomics: towards a high throughput monoclonal antibody-based resolution of proteins for diagnosis of mitochondrial diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Murray,Sky Yonally,Robert Aggeler,Michael F Marusich,Roderick A CapaldiPubMed 15576053
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Cow | Tested | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info 100 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 mg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (PubMed:21586654, PubMed:27693233). Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity). In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity (PubMed:31883789). Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis (By similarity). Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A4/ANT1 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity) (By similarity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it (By similarity). Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death (PubMed:31883789). It is however unclear if SLC25A4/ANT1 constitutes a pore-forming component of mPTP or regulates it (By similarity). Acts as a regulator of mitophagy independently of ADP:ATP antiporter activity: promotes mitophagy via interaction with TIMM44, leading to inhibit the presequence translocase TIMM23, thereby promoting stabilization of PINK1 (By similarity).
Additional Targets
SLC25A5, SLC25A6, SLC25A4
Alternative names
AAC1, ANT1, SLC25A4, ADP/ATP translocase 1, Adenine nucleotide translocator 1, Solute carrier family 25 member 4, ANT 1
Recommended products
Mouse Monoclonal ADT1 antibody. Suitable for IP, IHC-P and reacts with Cow, Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 5F51BB5AG7
- Light chain type
- kappa
- Concentration
- Purification notes
ab109864 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Adenine nucleotide translocase (ANT) also known as ADP/ATP translocase is a protein that mechanically facilitates the exchange of ATP and ADP across the inner mitochondrial membrane. This exchange is essential for maintaining the mitochondrial membrane potential and energy metabolism. ANT has a molecular mass of approximately 30-33 kDa and is expressed in the mitochondria of virtually all eukaryotic cells where it plays an essential role in energy homeostasis.
- Biological function summary
ANT functions as a transporter that is part of the mitochondrial permeability transition pore complex (mPTP). This complex plays an important role in regulating mitochondrial membrane permeability which affects processes like cell death and cellular metabolism. ANT's activity is a significant factor in regulating the mitochondrial functions since it directly influences the ATP supply to the cell.
- Pathways
ANT is a core component of the oxidative phosphorylation pathway which is an important biological process for ATP production. In this pathway ANT cooperates closely with other proteins such as cytochrome c oxidase and ATP synthase to ensure efficient ATP/ADP exchange and energy production. Additionally ANT's interaction with the mPTP implicates it in the apoptotic pathway where it can influence cell survival and death decisions.
- Associated diseases and disorders
Dysfunctional ANT is linked to conditions such as ischemia-reperfusion injury and neurodegenerative diseases. In ischemia-reperfusion injury ANT's role in mPTP formation is important as excessive mPTP opening can lead to cell death. Also mutations in ANT are associated with mitochondrial myopathy and cardiomyopathy where ANT's impaired function affects cardiac and muscle energy metabolism. ANT's relationship with the voltage-dependent anion channel (VDAC) further emphasizes its role in these disease pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864), expandable thumbnail](https://content.abcam.com/products/images/adenine-nucleotide-translocase-immunocapture-antibody-5f51bb5ag7-ab109864--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img34895.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864)
IHC image of Adenine Nucleotide Translocase staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109864, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Immunoprecipitation - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864), expandable thumbnail](https://content.abcam.com/products/images/adenine-nucleotide-translocase-immunocapture-antibody-5f51bb5ag7-ab109864--immunoprecipitation-img161838.jpg "Immunoprecipitation - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864)")
Immunoprecipitation - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864)
Immunocapture of Adenine Nucleotide Translocase from Bovine heart mitochondria using ab109864 at a concentration of 100 µg/mg lysate.
All lanes: Immunoprecipitation - Anti-Adenine Nucleotide Translocase Immunocapture antibody [5F51BB5AG7] (ab109864)
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