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AB220408

Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody

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(1 Publication)

Rabbit Polyclonal Adenine Nucleotide Translocator 2/ANT 2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLC25A5 aa 1-50.

View Alternative Names

AAC2, ANT2, SLC25A5, ADP/ATP translocase 2, Adenine nucleotide translocator 2, Solute carrier family 25 member 5, ANT 2, AAC1, ANT1, SLC25A4, ADP/ATP translocase 1, Adenine nucleotide translocator 1, Solute carrier family 25 member 4, ANT 1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (AB220408)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (AB220408)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized PC-3 cells labeling Adenine Nucleotide Translocator 2 with ab220408 at 4 μg/ml (green).

Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (AB220408)
  • WB

Supplier Data

Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (AB220408)

All lanes:

Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab220408) at 1/100 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Predicted band size: 33 kDa

false

Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (AB220408)
  • WB

Lab

Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (AB220408)

False colour image of Western blot : Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody staining at 1 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab220408 was shown to bind specifically to Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

Lanes 1 - 8:

Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (<a href='/en-us/products/primary-antibodies/adenine-nucleotide-translocator-1-2-ant-1-2-antibody-ab221956'>ab221956</a>) at 0.4 µg/mL

Lanes 1 - 8:

Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab220408) at 1 µg/mL

Lane 1:

SLC25A4 (Human) Recombinant Protein (ANT1) at 0.1 µg

Lane 2:

Recombinant Human Adenine Nucleotide Translocator 2/ANT 2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-adenine-nucleotide-translocator-2-ant-2-protein-ab157891'>ab157891</a>) at 0.1 µg

Lane 3:

Human Skeletal Muscle tissue lysate at 20 µg

Lane 4:

Human Liver tissue lysate at 20 µg

Lane 5:

Mouse Skeletal Muscle tissue lysate at 20 µg

Lane 6:

Mouse Liver tissue lysate at 20 µg

Lane 7:

Rat Skeletal Muscle tissue lysate at 20 µg

Lane 8:

Rat Liver tissue lysate at 20 µg

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (AB220408)
  • WB

Supplier Data

Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (AB220408)

All lanes:

Western blot - Anti-Adenine Nucleotide Translocator 1 + 2/ANT 1 + 2 antibody (ab220408) at 0.4 µg

All lanes:

Testis tissue lysate

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SLC25A5 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P05141

Specificity

This antibody can also recognise ADP/ATP translocase 2, SLC25A31, SLC25A5 and SLC25A6 due to homology of 93% in immunogen sequence.

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB110330

Anti-PDHA1 antibody [9H9AF5]

KO Validated

Western blot - Anti-PDHA1 antibody [9H9AF5] (AB110330)

  • 5
  • 8
Primary Antibodies

AB169540

Anti-Ndufs1 antibody [EPR11521(B)]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11521(B)] (AB169540)

  • 5
  • 2
Primary Antibodies

AB14748

Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (AB14748)

  • 5
  • 27
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Adenine Nucleotide Translocator 2 (ANT 2) and Adenine Nucleotide Translocase 1 (ANT 1) play an important role in mitochondrial energy metabolism. These proteins also referred to as ANT/anti and ANT 1/2 have a mass around 33 kDa each. They are integral components of the inner mitochondrial membrane and widely expressed in various tissues being abundant in those with high-energy demands such as heart and muscle tissues. ANT 2 and ANT 1 facilitate the exchange of ADP and ATP across the mitochondrial membrane critical for cellular energy supply.
Biological function summary

ANT 2 and ANT 1 contribute to maintaining mitochondrial function and integrity. They form part of the larger mitochondrial permeability transition pore complex which plays a role in energy metabolism and apoptosis. By regulating ADP/ATP exchange ANT 2 and ANT 1 ensure efficient energy production aligning cellular energy consumption with metabolic demands. Dysfunctions in their activity can result in impaired ATP synthesis affecting overall cellular viability and function.

Pathways

ANT 2 and ANT 1 participate significantly in critical processes like oxidative phosphorylation and the apoptosis pathway. In oxidative phosphorylation these proteins interact with the F1F0 ATP synthase complex to facilitate ATP production. Within the apoptosis pathway involvement in the opening of the mitochondrial transition pore can trigger programmed cell death. Connections with proteins such as cytochrome c occur in these pathways highlighting their role in energy regulation and cell survival.

Defects in ANT 2 and ANT 1 associate with conditions such as mitochondrial myopathy and dilated cardiomyopathy. Dysfunctional ANT proteins can disrupt mitochondrial homeostasis leading to energy deficiency and cell damage. They connect further to proteins like cytochrome c in apoptosis contributing to disease pathogenesis through altered cell death mechanisms. Investigating these proteins helps understand their role in disease development and offers potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ADP : ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (By similarity). Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state) : operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity). In addition to its ADP : ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity (By similarity). Plays a role in mitochondrial uncoupling by acting as a proton transporter : proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis (By similarity). Proton transporter activity is inhibited by ADP : ATP antiporter activity, suggesting that SLC25A5/ANT2 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP : ATP antiporter activity) and thermogenesis (proton transporter activity) (By similarity). Proton transporter activity requires free fatty acids as cofactor, but does not transport it (By similarity). Probably mediates mitochondrial uncoupling in tissues that do not express UCP1 (By similarity). Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death (PubMed : 31883789). It is however unclear if SLC25A5/ANT2 constitutes a pore-forming component of mPTP or regulates it (By similarity). Acts as a regulator of mitophagy independently of ADP : ATP antiporter activity : promotes mitophagy via interaction with TIMM44, leading to inhibit the presequence translocase TIMM23, thereby promoting stabilization of PINK1 (By similarity). As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (PubMed : 20797633).
See full target information SLC25A5

Additional targets

SLC25A4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:3448-3467 PubMed40328966

2025

CHD8 interacts with BCL11A to induce oncogenic transcription in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Waterhouse,Kyren Lazarus,Maria Francesca Santolla,Sara Pensa,Eleanor Williams,Abigail J Q Siu,Hisham Mohammed,Irina Mohorianu,Marcello Maggiolini,Jason Carroll,Laura S Itzhaki,Taufiq Rahman,Walid T Khaled
View all publications
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